Single-molecule orientation measurements with a quadrated pupil

This Letter presents a means of measuring the dipole orientation of a fluorescent, orientationally fixed single molecule, which uses a specially designed phase mask, termed a "quadrated pupil," conjugate to the back focal plane of a conventional wide-field microscope. The method leverages...

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Veröffentlicht in:	Optics letters 2013-05, Vol.38 (9), p.1521-1523
Hauptverfasser:	Backer, Adam S, Backlund, Mikael P, Lew, Matthew D, Moerner, W E
Format:	Artikel
Sprache:	eng
Schlagworte:	Anisotropy Dipoles Focal plane Masks Optical Imaging - methods Optical Phenomena Orientation Pupils Quantitative analysis
Online-Zugang:	Volltext
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