CpG-oligodeoxynucleotides suppress the proliferation of A549 lung adenocarcinoma cells via toll-like receptor 9 signaling and upregulation of Runt-related transcription factor 3 expression
The aim of the present study was to investigate the effect of CpG-oligodeoxynucelotides (CpG-ODN) on the proliferation of the A549 human lung adenocarcinoma cell line and the expression of Runt-related transcription factor 3 (Runx3) and investigate the association between the toll-like receptor 9 (T...
Gespeichert in:
| Veröffentlicht in: | Biomedical reports 2014-05, Vol.2 (3), p.374-377 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 377 |
|---|---|
| container_issue | 3 |
| container_start_page | 374 |
| container_title | Biomedical reports |
| container_volume | 2 |
| creator | BARNIE, PRINCE AMOAH ZHANG, PAN LU, PING CHEN, XIAOBO SU, ZHAOLIANG WANG, SHENGJUN XU, HUAXI |
| description | The aim of the present study was to investigate the effect of CpG-oligodeoxynucelotides (CpG-ODN) on the proliferation of the A549 human lung adenocarcinoma cell line and the expression of Runt-related transcription factor 3 (Runx3) and investigate the association between the toll-like receptor 9 (TLR9) signaling pathway and Runx3 expression during A549 cell proliferation. Different concentrations of CpG-ODN were used in this study to stimulate A549 cells and the expression of Runx3 at the mRNA or protein level was detected by reverse transcription-polymerase chain reaction or western blot analysis. Moreover, Runx3 siRNA was synthesized and transiently transfected into the A549 cells and the MTT assay was used to detect the effects of CpG-ODN on transfected cell growth. Our data demonstrated that CpG-ODN significantly inhibited the proliferation of A549 cells. The expression of Runx3 in the mRNA and protein level was increased in A549 cells stimulated by CpG-ODN. The CpG-ODN-stimulated cell proliferation was significantly inhibited in Runx3 siRNA-transfected A549 cells. In conclusion, CpG-ODN may bind to TLR9, inhibit the proliferation of A549 cells and upregulate the expression of Runx3. |
| doi_str_mv | 10.3892/br.2014.257 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3990193</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A406116465</galeid><sourcerecordid>A406116465</sourcerecordid><originalsourceid>FETCH-LOGICAL-c437t-b8dffc1deb6a0bf9546932b4888018ee84bdcfdc1b922f6576f8cd6699841d063</originalsourceid><addsrcrecordid>eNptklFrFDEQxxdRbKl98l0CggiyZzbJZjcvwlG0CgVB9Dlkk8k2NZesyW5pv5sfzmyvnlZMAgmT3_xnMpmqet7gDe0FeTukDcEN25C2e1QdE8xELRgjjw9nyo6q05yvcBmiw6Ttn1ZHhHWsF113XP08m87r6N0YDcSb27BoD3F2BjLKyzQlyBnNl4CmVCALSc0uBhQt2rZMIL-EESkDIWqVtAtxp5AG7zO6dgrN0fvau--AEmiY5piQQNmNQXm3-gWDlhJhXPxB9csS5jpBMYBBc1Ih6-Smu1ur9KpAEdzcpVVsz6onVvkMp_f7SfXtw_uvZx_ri8_nn862F7VmtJvroTfW6sbAwBUerGgZF5QMrO973PQAPRuMtkY3gyDE8rbjtteGcyF61hjM6Un1bq87LcMOjIZQUvNySm6n0q2MysmHN8FdyjFeSyoEbgQtAq_vBVL8sUCe5c7ltVAqQFyybHrC2xKcr7Fe_oNexSWVkhVKUCxIy2j7hxqVB-mCjSWuXkXllmHeNJzxldr8hyrTwM7pGMC6Yn_g8GbvoFPMOYE9vLHBcu03OSS59pss_VboF3-X5cD-7q4CvNoDeSqf7UzMB2ZINSY1pmV1jP4C74XhIw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1930925435</pqid></control><display><type>article</type><title>CpG-oligodeoxynucleotides suppress the proliferation of A549 lung adenocarcinoma cells via toll-like receptor 9 signaling and upregulation of Runt-related transcription factor 3 expression</title><source>Spandidos Publications Journals</source><source>PubMed Central</source><source>EZB Electronic Journals Library</source><creator>BARNIE, PRINCE AMOAH ; ZHANG, PAN ; LU, PING ; CHEN, XIAOBO ; SU, ZHAOLIANG ; WANG, SHENGJUN ; XU, HUAXI</creator><creatorcontrib>BARNIE, PRINCE AMOAH ; ZHANG, PAN ; LU, PING ; CHEN, XIAOBO ; SU, ZHAOLIANG ; WANG, SHENGJUN ; XU, HUAXI</creatorcontrib><description>The aim of the present study was to investigate the effect of CpG-oligodeoxynucelotides (CpG-ODN) on the proliferation of the A549 human lung adenocarcinoma cell line and the expression of Runt-related transcription factor 3 (Runx3) and investigate the association between the toll-like receptor 9 (TLR9) signaling pathway and Runx3 expression during A549 cell proliferation. Different concentrations of CpG-ODN were used in this study to stimulate A549 cells and the expression of Runx3 at the mRNA or protein level was detected by reverse transcription-polymerase chain reaction or western blot analysis. Moreover, Runx3 siRNA was synthesized and transiently transfected into the A549 cells and the MTT assay was used to detect the effects of CpG-ODN on transfected cell growth. Our data demonstrated that CpG-ODN significantly inhibited the proliferation of A549 cells. The expression of Runx3 in the mRNA and protein level was increased in A549 cells stimulated by CpG-ODN. The CpG-ODN-stimulated cell proliferation was significantly inhibited in Runx3 siRNA-transfected A549 cells. In conclusion, CpG-ODN may bind to TLR9, inhibit the proliferation of A549 cells and upregulate the expression of Runx3.</description><identifier>ISSN: 2049-9434</identifier><identifier>EISSN: 2049-9442</identifier><identifier>DOI: 10.3892/br.2014.257</identifier><identifier>PMID: 24748977</identifier><language>eng</language><publisher>England: D.A. Spandidos</publisher><subject>A549 cancer cells ; Adenocarcinoma ; Apoptosis ; Cell growth ; Cell proliferation ; Chemical synthesis ; CpG islands ; CpG-oligodeoxynucleotides ; Deoxyribonucleic acid ; Development and progression ; DNA ; Gastric cancer ; Gene expression ; Genetic aspects ; Health aspects ; Immune system ; Investigations ; Lung cancer ; Metastasis ; Oligodeoxynucleotides ; Oligonucleotides ; Polymerase chain reaction ; proliferation ; Proteins ; Reverse transcription ; Runt-related transcription factor 3 ; Runx3 protein ; Signal transduction ; siRNA ; TLR9 protein ; toll-like receptor 9 ; Toll-like receptors ; Transcription factors ; Tumorigenesis</subject><ispartof>Biomedical reports, 2014-05, Vol.2 (3), p.374-377</ispartof><rights>Copyright © 2014, Spandidos Publications</rights><rights>COPYRIGHT 2014 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2014</rights><rights>Copyright © 2014, Spandidos Publications 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-b8dffc1deb6a0bf9546932b4888018ee84bdcfdc1b922f6576f8cd6699841d063</citedby><cites>FETCH-LOGICAL-c437t-b8dffc1deb6a0bf9546932b4888018ee84bdcfdc1b922f6576f8cd6699841d063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990193/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990193/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,5556,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24748977$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BARNIE, PRINCE AMOAH</creatorcontrib><creatorcontrib>ZHANG, PAN</creatorcontrib><creatorcontrib>LU, PING</creatorcontrib><creatorcontrib>CHEN, XIAOBO</creatorcontrib><creatorcontrib>SU, ZHAOLIANG</creatorcontrib><creatorcontrib>WANG, SHENGJUN</creatorcontrib><creatorcontrib>XU, HUAXI</creatorcontrib><title>CpG-oligodeoxynucleotides suppress the proliferation of A549 lung adenocarcinoma cells via toll-like receptor 9 signaling and upregulation of Runt-related transcription factor 3 expression</title><title>Biomedical reports</title><addtitle>Biomed Rep</addtitle><description>The aim of the present study was to investigate the effect of CpG-oligodeoxynucelotides (CpG-ODN) on the proliferation of the A549 human lung adenocarcinoma cell line and the expression of Runt-related transcription factor 3 (Runx3) and investigate the association between the toll-like receptor 9 (TLR9) signaling pathway and Runx3 expression during A549 cell proliferation. Different concentrations of CpG-ODN were used in this study to stimulate A549 cells and the expression of Runx3 at the mRNA or protein level was detected by reverse transcription-polymerase chain reaction or western blot analysis. Moreover, Runx3 siRNA was synthesized and transiently transfected into the A549 cells and the MTT assay was used to detect the effects of CpG-ODN on transfected cell growth. Our data demonstrated that CpG-ODN significantly inhibited the proliferation of A549 cells. The expression of Runx3 in the mRNA and protein level was increased in A549 cells stimulated by CpG-ODN. The CpG-ODN-stimulated cell proliferation was significantly inhibited in Runx3 siRNA-transfected A549 cells. In conclusion, CpG-ODN may bind to TLR9, inhibit the proliferation of A549 cells and upregulate the expression of Runx3.</description><subject>A549 cancer cells</subject><subject>Adenocarcinoma</subject><subject>Apoptosis</subject><subject>Cell growth</subject><subject>Cell proliferation</subject><subject>Chemical synthesis</subject><subject>CpG islands</subject><subject>CpG-oligodeoxynucleotides</subject><subject>Deoxyribonucleic acid</subject><subject>Development and progression</subject><subject>DNA</subject><subject>Gastric cancer</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Immune system</subject><subject>Investigations</subject><subject>Lung cancer</subject><subject>Metastasis</subject><subject>Oligodeoxynucleotides</subject><subject>Oligonucleotides</subject><subject>Polymerase chain reaction</subject><subject>proliferation</subject><subject>Proteins</subject><subject>Reverse transcription</subject><subject>Runt-related transcription factor 3</subject><subject>Runx3 protein</subject><subject>Signal transduction</subject><subject>siRNA</subject><subject>TLR9 protein</subject><subject>toll-like receptor 9</subject><subject>Toll-like receptors</subject><subject>Transcription factors</subject><subject>Tumorigenesis</subject><issn>2049-9434</issn><issn>2049-9442</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNptklFrFDEQxxdRbKl98l0CggiyZzbJZjcvwlG0CgVB9Dlkk8k2NZesyW5pv5sfzmyvnlZMAgmT3_xnMpmqet7gDe0FeTukDcEN25C2e1QdE8xELRgjjw9nyo6q05yvcBmiw6Ttn1ZHhHWsF113XP08m87r6N0YDcSb27BoD3F2BjLKyzQlyBnNl4CmVCALSc0uBhQt2rZMIL-EESkDIWqVtAtxp5AG7zO6dgrN0fvau--AEmiY5piQQNmNQXm3-gWDlhJhXPxB9csS5jpBMYBBc1Ih6-Smu1ur9KpAEdzcpVVsz6onVvkMp_f7SfXtw_uvZx_ri8_nn862F7VmtJvroTfW6sbAwBUerGgZF5QMrO973PQAPRuMtkY3gyDE8rbjtteGcyF61hjM6Un1bq87LcMOjIZQUvNySm6n0q2MysmHN8FdyjFeSyoEbgQtAq_vBVL8sUCe5c7ltVAqQFyybHrC2xKcr7Fe_oNexSWVkhVKUCxIy2j7hxqVB-mCjSWuXkXllmHeNJzxldr8hyrTwM7pGMC6Yn_g8GbvoFPMOYE9vLHBcu03OSS59pss_VboF3-X5cD-7q4CvNoDeSqf7UzMB2ZINSY1pmV1jP4C74XhIw</recordid><startdate>20140501</startdate><enddate>20140501</enddate><creator>BARNIE, PRINCE AMOAH</creator><creator>ZHANG, PAN</creator><creator>LU, PING</creator><creator>CHEN, XIAOBO</creator><creator>SU, ZHAOLIANG</creator><creator>WANG, SHENGJUN</creator><creator>XU, HUAXI</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140501</creationdate><title>CpG-oligodeoxynucleotides suppress the proliferation of A549 lung adenocarcinoma cells via toll-like receptor 9 signaling and upregulation of Runt-related transcription factor 3 expression</title><author>BARNIE, PRINCE AMOAH ; ZHANG, PAN ; LU, PING ; CHEN, XIAOBO ; SU, ZHAOLIANG ; WANG, SHENGJUN ; XU, HUAXI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-b8dffc1deb6a0bf9546932b4888018ee84bdcfdc1b922f6576f8cd6699841d063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>A549 cancer cells</topic><topic>Adenocarcinoma</topic><topic>Apoptosis</topic><topic>Cell growth</topic><topic>Cell proliferation</topic><topic>Chemical synthesis</topic><topic>CpG islands</topic><topic>CpG-oligodeoxynucleotides</topic><topic>Deoxyribonucleic acid</topic><topic>Development and progression</topic><topic>DNA</topic><topic>Gastric cancer</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Immune system</topic><topic>Investigations</topic><topic>Lung cancer</topic><topic>Metastasis</topic><topic>Oligodeoxynucleotides</topic><topic>Oligonucleotides</topic><topic>Polymerase chain reaction</topic><topic>proliferation</topic><topic>Proteins</topic><topic>Reverse transcription</topic><topic>Runt-related transcription factor 3</topic><topic>Runx3 protein</topic><topic>Signal transduction</topic><topic>siRNA</topic><topic>TLR9 protein</topic><topic>toll-like receptor 9</topic><topic>Toll-like receptors</topic><topic>Transcription factors</topic><topic>Tumorigenesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BARNIE, PRINCE AMOAH</creatorcontrib><creatorcontrib>ZHANG, PAN</creatorcontrib><creatorcontrib>LU, PING</creatorcontrib><creatorcontrib>CHEN, XIAOBO</creatorcontrib><creatorcontrib>SU, ZHAOLIANG</creatorcontrib><creatorcontrib>WANG, SHENGJUN</creatorcontrib><creatorcontrib>XU, HUAXI</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Biological Sciences</collection><collection>Biological Science Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biomedical reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BARNIE, PRINCE AMOAH</au><au>ZHANG, PAN</au><au>LU, PING</au><au>CHEN, XIAOBO</au><au>SU, ZHAOLIANG</au><au>WANG, SHENGJUN</au><au>XU, HUAXI</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CpG-oligodeoxynucleotides suppress the proliferation of A549 lung adenocarcinoma cells via toll-like receptor 9 signaling and upregulation of Runt-related transcription factor 3 expression</atitle><jtitle>Biomedical reports</jtitle><addtitle>Biomed Rep</addtitle><date>2014-05-01</date><risdate>2014</risdate><volume>2</volume><issue>3</issue><spage>374</spage><epage>377</epage><pages>374-377</pages><issn>2049-9434</issn><eissn>2049-9442</eissn><abstract>The aim of the present study was to investigate the effect of CpG-oligodeoxynucelotides (CpG-ODN) on the proliferation of the A549 human lung adenocarcinoma cell line and the expression of Runt-related transcription factor 3 (Runx3) and investigate the association between the toll-like receptor 9 (TLR9) signaling pathway and Runx3 expression during A549 cell proliferation. Different concentrations of CpG-ODN were used in this study to stimulate A549 cells and the expression of Runx3 at the mRNA or protein level was detected by reverse transcription-polymerase chain reaction or western blot analysis. Moreover, Runx3 siRNA was synthesized and transiently transfected into the A549 cells and the MTT assay was used to detect the effects of CpG-ODN on transfected cell growth. Our data demonstrated that CpG-ODN significantly inhibited the proliferation of A549 cells. The expression of Runx3 in the mRNA and protein level was increased in A549 cells stimulated by CpG-ODN. The CpG-ODN-stimulated cell proliferation was significantly inhibited in Runx3 siRNA-transfected A549 cells. In conclusion, CpG-ODN may bind to TLR9, inhibit the proliferation of A549 cells and upregulate the expression of Runx3.</abstract><cop>England</cop><pub>D.A. Spandidos</pub><pmid>24748977</pmid><doi>10.3892/br.2014.257</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2049-9434 |
| ispartof | Biomedical reports, 2014-05, Vol.2 (3), p.374-377 |
| issn | 2049-9434 2049-9442 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3990193 |
| source | Spandidos Publications Journals; PubMed Central; EZB Electronic Journals Library |
| subjects | A549 cancer cells Adenocarcinoma Apoptosis Cell growth Cell proliferation Chemical synthesis CpG islands CpG-oligodeoxynucleotides Deoxyribonucleic acid Development and progression DNA Gastric cancer Gene expression Genetic aspects Health aspects Immune system Investigations Lung cancer Metastasis Oligodeoxynucleotides Oligonucleotides Polymerase chain reaction proliferation Proteins Reverse transcription Runt-related transcription factor 3 Runx3 protein Signal transduction siRNA TLR9 protein toll-like receptor 9 Toll-like receptors Transcription factors Tumorigenesis |
| title | CpG-oligodeoxynucleotides suppress the proliferation of A549 lung adenocarcinoma cells via toll-like receptor 9 signaling and upregulation of Runt-related transcription factor 3 expression |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T21%3A49%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=CpG-oligodeoxynucleotides%20suppress%20the%20proliferation%20of%20A549%20lung%20adenocarcinoma%20cells%20via%20toll-like%20receptor%209%20signaling%20and%20upregulation%20of%20Runt-related%20transcription%20factor%203%20expression&rft.jtitle=Biomedical%20reports&rft.au=BARNIE,%20PRINCE%20AMOAH&rft.date=2014-05-01&rft.volume=2&rft.issue=3&rft.spage=374&rft.epage=377&rft.pages=374-377&rft.issn=2049-9434&rft.eissn=2049-9442&rft_id=info:doi/10.3892/br.2014.257&rft_dat=%3Cgale_pubme%3EA406116465%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1930925435&rft_id=info:pmid/24748977&rft_galeid=A406116465&rfr_iscdi=true |