Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens
Fowl adenovirus (FAdv) serotype 2 causes inclusion body hepatitis (IBH) disease which adversely affects the broiler industry in Thailand. We developed an indirect ELISA based on the recombinant hexon protein produced by E. coli. The recombinant hexon protein was tested with sera, in both infected an...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2014, Vol.76(2), pp.289-293 |
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| container_title | Journal of Veterinary Medical Science |
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| creator | JUNNU, Sucheeva LERTWATCHARASARAKUL, Preeda JALA, Siriluk PHATTANAKUNANAN, Sakuna MOONJIT, Pattra SONGSERM, Thaweesak |
| description | Fowl adenovirus (FAdv) serotype 2 causes inclusion body hepatitis (IBH) disease which adversely affects the broiler industry in Thailand. We developed an indirect ELISA based on the recombinant hexon protein produced by E. coli. The recombinant hexon protein was tested with sera, in both infected and noninfected chickens. The recombinant hexon protein was standardized with an antigen concentration of 3.75 µg/ml and test sera. The intra- and inter-assays were repeatable. The cutoff value from TG-ROC curve analysis was 0.106. The specificity and sensitivity were 80 and 80%, respectively. The correlation coefficient (r) of absorbance values from this ELISA compared with the serum neutralization test was 0.76. This ELISA might be helpful for IBH diagnosis and surveillance. |
| doi_str_mv | 10.1292/jvms.13-0196 |
| format | Article |
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We developed an indirect ELISA based on the recombinant hexon protein produced by E. coli. The recombinant hexon protein was tested with sera, in both infected and noninfected chickens. The recombinant hexon protein was standardized with an antigen concentration of 3.75 µg/ml and test sera. The intra- and inter-assays were repeatable. The cutoff value from TG-ROC curve analysis was 0.106. The specificity and sensitivity were 80 and 80%, respectively. The correlation coefficient (r) of absorbance values from this ELISA compared with the serum neutralization test was 0.76. This ELISA might be helpful for IBH diagnosis and surveillance.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.13-0196</identifier><identifier>PMID: 24141320</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Adenoviridae Infections - diagnosis ; Adenoviridae Infections - veterinary ; Animals ; Capsid Proteins - genetics ; Chickens ; Electrophoresis, Polyacrylamide Gel - veterinary ; enzyme-linked immunosorbent assay (ELISA) ; Enzyme-Linked Immunosorbent Assay - methods ; Enzyme-Linked Immunosorbent Assay - veterinary ; fowl adenovirus ; Hepatitis, Viral, Animal - diagnosis ; Inclusion Bodies, Viral ; inclusion body hepatitis ; Poultry Diseases - diagnosis ; Poultry Diseases - virology ; recombinant hexon protein ; Recombinant Proteins - genetics ; ROC Curve ; Serologic Tests - methods ; Serologic Tests - veterinary ; Virology</subject><ispartof>Journal of Veterinary Medical Science, 2014, Vol.76(2), pp.289-293</ispartof><rights>2014 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 2014</rights><rights>2014 The Japanese Society of Veterinary Science 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c689t-f0d6dd1dafa04ba2e3aabc54770f95c2be0c9ec4e9dcef94f23b5cc0e8a2feb3</citedby><cites>FETCH-LOGICAL-c689t-f0d6dd1dafa04ba2e3aabc54770f95c2be0c9ec4e9dcef94f23b5cc0e8a2feb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982830/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982830/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1877,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24141320$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JUNNU, Sucheeva</creatorcontrib><creatorcontrib>LERTWATCHARASARAKUL, Preeda</creatorcontrib><creatorcontrib>JALA, Siriluk</creatorcontrib><creatorcontrib>PHATTANAKUNANAN, Sakuna</creatorcontrib><creatorcontrib>MOONJIT, Pattra</creatorcontrib><creatorcontrib>SONGSERM, Thaweesak</creatorcontrib><title>Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>Fowl adenovirus (FAdv) serotype 2 causes inclusion body hepatitis (IBH) disease which adversely affects the broiler industry in Thailand. We developed an indirect ELISA based on the recombinant hexon protein produced by E. coli. The recombinant hexon protein was tested with sera, in both infected and noninfected chickens. The recombinant hexon protein was standardized with an antigen concentration of 3.75 µg/ml and test sera. The intra- and inter-assays were repeatable. The cutoff value from TG-ROC curve analysis was 0.106. The specificity and sensitivity were 80 and 80%, respectively. The correlation coefficient (r) of absorbance values from this ELISA compared with the serum neutralization test was 0.76. This ELISA might be helpful for IBH diagnosis and surveillance.</description><subject>Adenoviridae Infections - diagnosis</subject><subject>Adenoviridae Infections - veterinary</subject><subject>Animals</subject><subject>Capsid Proteins - genetics</subject><subject>Chickens</subject><subject>Electrophoresis, Polyacrylamide Gel - veterinary</subject><subject>enzyme-linked immunosorbent assay (ELISA)</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>fowl adenovirus</subject><subject>Hepatitis, Viral, Animal - diagnosis</subject><subject>Inclusion Bodies, Viral</subject><subject>inclusion body hepatitis</subject><subject>Poultry Diseases - diagnosis</subject><subject>Poultry Diseases - virology</subject><subject>recombinant hexon protein</subject><subject>Recombinant Proteins - genetics</subject><subject>ROC Curve</subject><subject>Serologic Tests - methods</subject><subject>Serologic Tests - veterinary</subject><subject>Virology</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc9v0zAUxyMEYmVw44wsceFAhn8lqS9IazdYpUogtrvlOM-tS2p3tlOx4_7zObRUwMWW_T7vIz9_i-ItwReECvpps9_GC8JKTET9rJgQxpuy4Uw8LyZYkLpsaIXPilcxbjCmhNfiZXFGOeGEUTwpHq9gD73fWbdCyqGF62wAndD1cnF7iWYqQoe8Qz9A-21rnXIJ3cCvfPM9-ATWIeMDuoXge7-yWvXoClLut5nwJut0P8TxMPPdQ-7cqWSTjSg3ztdW_wQXXxcvjOojvDnu58Xdl-u7-U25_PZ1Mb9clrqeilQa3NVdRzplFOatosCUanXFmwYbUWnaAtYCNAfRaTCCG8raSmsMU0UNtOy8-HzQ7oZ2C5lxKahe7oLdqvAgvbLy34qza7nye8nElE4ZzoIPR0Hw9wPEJLc2auh75cAPUZIK518VFa8y-v4_dOOH4PJ0vylGqpqQTH08UDr4GAOY02MIlmO0coxWEibHaDP-7u8BTvCfLDMwOwCbmNQKToAKyeoeDramlnRcjtZTUa9VkODYExZfuzI</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>JUNNU, Sucheeva</creator><creator>LERTWATCHARASARAKUL, Preeda</creator><creator>JALA, Siriluk</creator><creator>PHATTANAKUNANAN, Sakuna</creator><creator>MOONJIT, Pattra</creator><creator>SONGSERM, Thaweesak</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2014</creationdate><title>Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens</title><author>JUNNU, Sucheeva ; LERTWATCHARASARAKUL, Preeda ; JALA, Siriluk ; PHATTANAKUNANAN, Sakuna ; MOONJIT, Pattra ; SONGSERM, Thaweesak</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c689t-f0d6dd1dafa04ba2e3aabc54770f95c2be0c9ec4e9dcef94f23b5cc0e8a2feb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adenoviridae Infections - diagnosis</topic><topic>Adenoviridae Infections - veterinary</topic><topic>Animals</topic><topic>Capsid Proteins - genetics</topic><topic>Chickens</topic><topic>Electrophoresis, Polyacrylamide Gel - veterinary</topic><topic>enzyme-linked immunosorbent assay (ELISA)</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>fowl adenovirus</topic><topic>Hepatitis, Viral, Animal - diagnosis</topic><topic>Inclusion Bodies, Viral</topic><topic>inclusion body hepatitis</topic><topic>Poultry Diseases - diagnosis</topic><topic>Poultry Diseases - virology</topic><topic>recombinant hexon protein</topic><topic>Recombinant Proteins - genetics</topic><topic>ROC Curve</topic><topic>Serologic Tests - methods</topic><topic>Serologic Tests - veterinary</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JUNNU, Sucheeva</creatorcontrib><creatorcontrib>LERTWATCHARASARAKUL, Preeda</creatorcontrib><creatorcontrib>JALA, Siriluk</creatorcontrib><creatorcontrib>PHATTANAKUNANAN, Sakuna</creatorcontrib><creatorcontrib>MOONJIT, Pattra</creatorcontrib><creatorcontrib>SONGSERM, Thaweesak</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JUNNU, Sucheeva</au><au>LERTWATCHARASARAKUL, Preeda</au><au>JALA, Siriluk</au><au>PHATTANAKUNANAN, Sakuna</au><au>MOONJIT, Pattra</au><au>SONGSERM, Thaweesak</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2014</date><risdate>2014</risdate><volume>76</volume><issue>2</issue><spage>289</spage><epage>293</epage><pages>289-293</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Fowl adenovirus (FAdv) serotype 2 causes inclusion body hepatitis (IBH) disease which adversely affects the broiler industry in Thailand. We developed an indirect ELISA based on the recombinant hexon protein produced by E. coli. The recombinant hexon protein was tested with sera, in both infected and noninfected chickens. The recombinant hexon protein was standardized with an antigen concentration of 3.75 µg/ml and test sera. The intra- and inter-assays were repeatable. The cutoff value from TG-ROC curve analysis was 0.106. The specificity and sensitivity were 80 and 80%, respectively. The correlation coefficient (r) of absorbance values from this ELISA compared with the serum neutralization test was 0.76. This ELISA might be helpful for IBH diagnosis and surveillance.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>24141320</pmid><doi>10.1292/jvms.13-0196</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of Veterinary Medical Science, 2014, Vol.76(2), pp.289-293 |
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| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Adenoviridae Infections - diagnosis Adenoviridae Infections - veterinary Animals Capsid Proteins - genetics Chickens Electrophoresis, Polyacrylamide Gel - veterinary enzyme-linked immunosorbent assay (ELISA) Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary fowl adenovirus Hepatitis, Viral, Animal - diagnosis Inclusion Bodies, Viral inclusion body hepatitis Poultry Diseases - diagnosis Poultry Diseases - virology recombinant hexon protein Recombinant Proteins - genetics ROC Curve Serologic Tests - methods Serologic Tests - veterinary Virology |
| title | Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens |
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