Organic small hairpin RNAs (OshR): A do-it-yourself platform for transgene-based gene silencing

The RNA interference (RNAi) pathway in animal cells can be harnessed to silence gene expression with artificial small interfering RNAs (siRNAs) or transgenes that express small hairpin RNAs (shRNAs). The transgene-expressing shRNA approach has been adapted into large-scale resources for genome-wide...

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Veröffentlicht in:	Methods (San Diego, Calif.) Calif.), 2013-09, Vol.63 (2), p.101-109
Hauptverfasser:	Zeng, Mei, Kuzirian, Marissa S., Harper, Lamia, Paradis, Suzanne, Nakayama, Takuya, Lau, Nelson C.
Format:	Artikel
Sprache:	eng
Schlagworte:	3' Untranslated Regions Animals Base Pairing Base Sequence Cloning, Molecular Gene Knockdown Techniques - methods Gene silencing HEK293 Cells Humans Mice MicroRNAs - genetics Molecular Sequence Data Monophenol Monooxygenase - biosynthesis Monophenol Monooxygenase - genetics NIMA-Related Kinases Protein Serine-Threonine Kinases - biosynthesis Protein Serine-Threonine Kinases - genetics RNA Interference RNA, Messenger - genetics RNA, Small Interfering - genetics shRNA Transgenes Xenopus Xenopus Proteins - biosynthesis Xenopus Proteins - genetics
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