Human adenovirus 36 decreases fatty acid oxidation and increases de novo lipogenesis in primary cultured human skeletal muscle cells by promoting Cidec/FSP27 expression
Background: It has been well documented that human adenovirus type 36 (Ad-36) is associated with obesity. However, the underlying molecular mechanism of Ad-36 inducing obesity remains unknown. We sought to investigate the effect of Ad-36 infection on Cidec, AMPK pathway and lipid metabolism in prima...
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| Veröffentlicht in: | International Journal of Obesity 2010-09, Vol.34 (9), p.1355-1364 |
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| description | Background: It has been well documented that human adenovirus type 36 (Ad-36) is associated with obesity. However, the underlying molecular mechanism of Ad-36 inducing obesity remains unknown. We sought to investigate the effect of Ad-36 infection on Cidec, AMPK pathway and lipid metabolism in primary cultured human skeletal muscle cells. Methods: Cidec/fat-specific protein 27 (FSP27), fatty acid oxidation, AMPK signaling and the abundance of proteins involved in lipid synthesis were determined in muscle cells infected with various doses (1.9–7.6 MOI) of Ad-36 and non-lipogenic adenovirus type 2 (Ad-2) as a negative control as well as an uninfected control. Cidec/FSP27 siRNA transfection was performed in Ad-36-infected muscle cells. Results: Our data show that Ad-36 significantly reduced fatty acid oxidation in a dose-dependent manner (all P values are |
| doi_str_mv | 10.1038/ijo.2010.77 |
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| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3962839</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A238094351</galeid><sourcerecordid>A238094351</sourcerecordid><originalsourceid>FETCH-LOGICAL-c596t-5bbbdf28b466dbbd9d8c4d88a52d75db73d45cd15ef249daeeda67686e5828ce3</originalsourceid><addsrcrecordid>eNptkk1v1DAQhiMEoqVw4g4WCDjAbh1_xMkFqVpRilQJpNKz5diTXS-OvdhJ1f1H_Ey87PZjUeNDbM8z76sZT1G8LPG0xLQ-tsswJTifhHhUHJZMVBPOGvG4OMQUiwnmFT8onqW0xBhzjsnT4oBgxjBpxGHx52zslUfKgA9XNo4J0QoZ0BFUgoQ6NQxrpLQ1KFxbowYbMuwNsv4GMYByakDOrsIcPCSbchStou1VXCM9umGMYNDin1H6BQ4G5VA_Ju0AaXAuoXad-dCHwfo5mtnsf3x68YMIBNerCCll1-fFk065BC92_6Pi8vTLz9nZ5Pz712-zk_OJ5k01THjbtqYjdcuqyuRtY2rNTF0rTozgphXUMK5NyaEjrDEKwKhKVHUFvCa1BnpUfN7qrsa2B6PBD1E5uStHBmXlfsTbhZyHK0mbitS0yQIfdgIx_B4hDbK3aVOm8hDGJAVnGBPGRCbf_Ecuwxh9ri5DmJa4ESxDb7fQXDmQ1nchu-qNpDwhtMYNo7zM1PQBKi8DvdXBQ2fz_V7C-3sJC1BuWKTgxs0Dp33w4xbUMaQUobttRYnlZv5knj-5mT8pNiW9ut-9W_Zm4DLwbgeopJXrovLapjuOEsryl7lPWy7lkJ9DvOvNw76vt3inglTzmCUvL3KQ4rIhuKlq-hd_tv2h</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>750310974</pqid></control><display><type>article</type><title>Human adenovirus 36 decreases fatty acid oxidation and increases de novo lipogenesis in primary cultured human skeletal muscle cells by promoting Cidec/FSP27 expression</title><source>MEDLINE</source><source>SpringerLink Journals</source><source>Nature Journals Online</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Wang, Z.Q ; Yu, Y ; Zhang, X.H ; Floyd, E.Z ; Cefalu, W.T</creator><creatorcontrib>Wang, Z.Q ; Yu, Y ; Zhang, X.H ; Floyd, E.Z ; Cefalu, W.T</creatorcontrib><description>Background: It has been well documented that human adenovirus type 36 (Ad-36) is associated with obesity. However, the underlying molecular mechanism of Ad-36 inducing obesity remains unknown. We sought to investigate the effect of Ad-36 infection on Cidec, AMPK pathway and lipid metabolism in primary cultured human skeletal muscle cells. Methods: Cidec/fat-specific protein 27 (FSP27), fatty acid oxidation, AMPK signaling and the abundance of proteins involved in lipid synthesis were determined in muscle cells infected with various doses (1.9–7.6 MOI) of Ad-36 and non-lipogenic adenovirus type 2 (Ad-2) as a negative control as well as an uninfected control. Cidec/FSP27 siRNA transfection was performed in Ad-36-infected muscle cells. Results: Our data show that Ad-36 significantly reduced fatty acid oxidation in a dose-dependent manner (all P values are <0.01), but Ad-2 did not affect fatty acid oxidation. Ad-36 substantially increased Cidec/FSP27, ACC, sterol regulatory element-binding protein 1c (SREBP-1c), SREBP-2 and 3-hydroxy-3-methylglutaryl-CoA reductase protein abundance, but significantly reduced AMPK activity, mitochondrial mass and uncoupling protein 3 (UCP3) abundance in comparison with control cells (all P values are <0.01). Oil Red O staining revealed that there was substantial fat accumulation in the Ad-36-infected muscle cells. Furthermore, Cidec/FSP27 siRNA transfection significantly reduced FSP27 expression and partially restored AMPK signaling, increased UCP3 and decreased SERBP 1c and perilipin proteins in Ad-36-infected muscle cells. Interestingly, neither Ad-36 nor Ad-2 affected peroxisome proliferator-activated receptor γ protein expression in muscle cells. Conclusion: This study suggests that Ad-36 induced lipid droplets in the cultured skeletal muscle cells and this process may be mediated by promoting Cidec/FSP27 expression.</description><identifier>ISSN: 0307-0565</identifier><identifier>EISSN: 1476-5497</identifier><identifier>DOI: 10.1038/ijo.2010.77</identifier><identifier>PMID: 20440297</identifier><identifier>CODEN: IJOBDP</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/443/319/2723 ; 631/80/86 ; 692/698/1671/1668/1973 ; 692/699/255/2514 ; Adenovirus diseases ; Adenoviruses ; Adenoviruses, Human - genetics ; Adenoviruses, Human - metabolism ; Adipocytes ; Biological and medical sciences ; Biological oxidation (Metabolism) ; Biomedical research ; Body fat ; cell culture ; Cells, Cultured ; Complications and side effects ; de novo lipogenesis ; Epidemiology ; fatty acid composition ; Fatty acids ; Fatty Acids - genetics ; Fatty Acids - metabolism ; Genetic aspects ; Glucose ; Health aspects ; Health Promotion and Disease Prevention ; Human adenovirus D ; human cell lines ; Humans ; Insulin resistance ; Internal Medicine ; Lipid Metabolism ; lipid peroxidation ; Lipids ; lipogenesis ; Lipogenesis - genetics ; Lipogenesis - physiology ; Medical sciences ; Medicine ; Medicine & Public Health ; Metabolic Diseases ; Metabolism ; Muscle cells ; Muscle, Skeletal - metabolism ; Muscle, Skeletal - virology ; Musculoskeletal system ; Obesity ; Obesity - genetics ; Obesity - metabolism ; Obesity - virology ; original-article ; Oxidation ; Oxidation-Reduction ; Physiological aspects ; Protein expression ; protein synthesis ; Proteins ; Proteins - genetics ; Proteins - metabolism ; Public Health ; Risk factors ; Signal Transduction ; skeletal muscle ; Triglycerides</subject><ispartof>International Journal of Obesity, 2010-09, Vol.34 (9), p.1355-1364</ispartof><rights>Macmillan Publishers Limited 2010</rights><rights>2015 INIST-CNRS</rights><rights>COPYRIGHT 2010 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Sep 2010</rights><rights>2010 Macmillan Publishers Limited All rights reserved 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c596t-5bbbdf28b466dbbd9d8c4d88a52d75db73d45cd15ef249daeeda67686e5828ce3</citedby><cites>FETCH-LOGICAL-c596t-5bbbdf28b466dbbd9d8c4d88a52d75db73d45cd15ef249daeeda67686e5828ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/ijo.2010.77$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/ijo.2010.77$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23234444$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20440297$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Z.Q</creatorcontrib><creatorcontrib>Yu, Y</creatorcontrib><creatorcontrib>Zhang, X.H</creatorcontrib><creatorcontrib>Floyd, E.Z</creatorcontrib><creatorcontrib>Cefalu, W.T</creatorcontrib><title>Human adenovirus 36 decreases fatty acid oxidation and increases de novo lipogenesis in primary cultured human skeletal muscle cells by promoting Cidec/FSP27 expression</title><title>International Journal of Obesity</title><addtitle>Int J Obes</addtitle><addtitle>Int J Obes (Lond)</addtitle><description>Background: It has been well documented that human adenovirus type 36 (Ad-36) is associated with obesity. However, the underlying molecular mechanism of Ad-36 inducing obesity remains unknown. We sought to investigate the effect of Ad-36 infection on Cidec, AMPK pathway and lipid metabolism in primary cultured human skeletal muscle cells. Methods: Cidec/fat-specific protein 27 (FSP27), fatty acid oxidation, AMPK signaling and the abundance of proteins involved in lipid synthesis were determined in muscle cells infected with various doses (1.9–7.6 MOI) of Ad-36 and non-lipogenic adenovirus type 2 (Ad-2) as a negative control as well as an uninfected control. Cidec/FSP27 siRNA transfection was performed in Ad-36-infected muscle cells. Results: Our data show that Ad-36 significantly reduced fatty acid oxidation in a dose-dependent manner (all P values are <0.01), but Ad-2 did not affect fatty acid oxidation. Ad-36 substantially increased Cidec/FSP27, ACC, sterol regulatory element-binding protein 1c (SREBP-1c), SREBP-2 and 3-hydroxy-3-methylglutaryl-CoA reductase protein abundance, but significantly reduced AMPK activity, mitochondrial mass and uncoupling protein 3 (UCP3) abundance in comparison with control cells (all P values are <0.01). Oil Red O staining revealed that there was substantial fat accumulation in the Ad-36-infected muscle cells. Furthermore, Cidec/FSP27 siRNA transfection significantly reduced FSP27 expression and partially restored AMPK signaling, increased UCP3 and decreased SERBP 1c and perilipin proteins in Ad-36-infected muscle cells. Interestingly, neither Ad-36 nor Ad-2 affected peroxisome proliferator-activated receptor γ protein expression in muscle cells. Conclusion: This study suggests that Ad-36 induced lipid droplets in the cultured skeletal muscle cells and this process may be mediated by promoting Cidec/FSP27 expression.</description><subject>631/443/319/2723</subject><subject>631/80/86</subject><subject>692/698/1671/1668/1973</subject><subject>692/699/255/2514</subject><subject>Adenovirus diseases</subject><subject>Adenoviruses</subject><subject>Adenoviruses, Human - genetics</subject><subject>Adenoviruses, Human - metabolism</subject><subject>Adipocytes</subject><subject>Biological and medical sciences</subject><subject>Biological oxidation (Metabolism)</subject><subject>Biomedical research</subject><subject>Body fat</subject><subject>cell culture</subject><subject>Cells, Cultured</subject><subject>Complications and side effects</subject><subject>de novo lipogenesis</subject><subject>Epidemiology</subject><subject>fatty acid composition</subject><subject>Fatty acids</subject><subject>Fatty Acids - genetics</subject><subject>Fatty Acids - metabolism</subject><subject>Genetic aspects</subject><subject>Glucose</subject><subject>Health aspects</subject><subject>Health Promotion and Disease Prevention</subject><subject>Human adenovirus D</subject><subject>human cell lines</subject><subject>Humans</subject><subject>Insulin resistance</subject><subject>Internal Medicine</subject><subject>Lipid Metabolism</subject><subject>lipid peroxidation</subject><subject>Lipids</subject><subject>lipogenesis</subject><subject>Lipogenesis - genetics</subject><subject>Lipogenesis - physiology</subject><subject>Medical sciences</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Metabolic Diseases</subject><subject>Metabolism</subject><subject>Muscle cells</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Muscle, Skeletal - virology</subject><subject>Musculoskeletal system</subject><subject>Obesity</subject><subject>Obesity - genetics</subject><subject>Obesity - metabolism</subject><subject>Obesity - virology</subject><subject>original-article</subject><subject>Oxidation</subject><subject>Oxidation-Reduction</subject><subject>Physiological aspects</subject><subject>Protein expression</subject><subject>protein synthesis</subject><subject>Proteins</subject><subject>Proteins - genetics</subject><subject>Proteins - metabolism</subject><subject>Public Health</subject><subject>Risk factors</subject><subject>Signal Transduction</subject><subject>skeletal muscle</subject><subject>Triglycerides</subject><issn>0307-0565</issn><issn>1476-5497</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptkk1v1DAQhiMEoqVw4g4WCDjAbh1_xMkFqVpRilQJpNKz5diTXS-OvdhJ1f1H_Ey87PZjUeNDbM8z76sZT1G8LPG0xLQ-tsswJTifhHhUHJZMVBPOGvG4OMQUiwnmFT8onqW0xBhzjsnT4oBgxjBpxGHx52zslUfKgA9XNo4J0QoZ0BFUgoQ6NQxrpLQ1KFxbowYbMuwNsv4GMYByakDOrsIcPCSbchStou1VXCM9umGMYNDin1H6BQ4G5VA_Ju0AaXAuoXad-dCHwfo5mtnsf3x68YMIBNerCCll1-fFk065BC92_6Pi8vTLz9nZ5Pz712-zk_OJ5k01THjbtqYjdcuqyuRtY2rNTF0rTozgphXUMK5NyaEjrDEKwKhKVHUFvCa1BnpUfN7qrsa2B6PBD1E5uStHBmXlfsTbhZyHK0mbitS0yQIfdgIx_B4hDbK3aVOm8hDGJAVnGBPGRCbf_Ecuwxh9ri5DmJa4ESxDb7fQXDmQ1nchu-qNpDwhtMYNo7zM1PQBKi8DvdXBQ2fz_V7C-3sJC1BuWKTgxs0Dp33w4xbUMaQUobttRYnlZv5knj-5mT8pNiW9ut-9W_Zm4DLwbgeopJXrovLapjuOEsryl7lPWy7lkJ9DvOvNw76vt3inglTzmCUvL3KQ4rIhuKlq-hd_tv2h</recordid><startdate>20100901</startdate><enddate>20100901</enddate><creator>Wang, Z.Q</creator><creator>Yu, Y</creator><creator>Zhang, X.H</creator><creator>Floyd, E.Z</creator><creator>Cefalu, W.T</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T2</scope><scope>7TK</scope><scope>7TS</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20100901</creationdate><title>Human adenovirus 36 decreases fatty acid oxidation and increases de novo lipogenesis in primary cultured human skeletal muscle cells by promoting Cidec/FSP27 expression</title><author>Wang, Z.Q ; Yu, Y ; Zhang, X.H ; Floyd, E.Z ; Cefalu, W.T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c596t-5bbbdf28b466dbbd9d8c4d88a52d75db73d45cd15ef249daeeda67686e5828ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>631/443/319/2723</topic><topic>631/80/86</topic><topic>692/698/1671/1668/1973</topic><topic>692/699/255/2514</topic><topic>Adenovirus diseases</topic><topic>Adenoviruses</topic><topic>Adenoviruses, Human - genetics</topic><topic>Adenoviruses, Human - metabolism</topic><topic>Adipocytes</topic><topic>Biological and medical sciences</topic><topic>Biological oxidation (Metabolism)</topic><topic>Biomedical research</topic><topic>Body fat</topic><topic>cell culture</topic><topic>Cells, Cultured</topic><topic>Complications and side effects</topic><topic>de novo lipogenesis</topic><topic>Epidemiology</topic><topic>fatty acid composition</topic><topic>Fatty acids</topic><topic>Fatty Acids - genetics</topic><topic>Fatty Acids - metabolism</topic><topic>Genetic aspects</topic><topic>Glucose</topic><topic>Health aspects</topic><topic>Health Promotion and Disease Prevention</topic><topic>Human adenovirus D</topic><topic>human cell lines</topic><topic>Humans</topic><topic>Insulin resistance</topic><topic>Internal Medicine</topic><topic>Lipid Metabolism</topic><topic>lipid peroxidation</topic><topic>Lipids</topic><topic>lipogenesis</topic><topic>Lipogenesis - genetics</topic><topic>Lipogenesis - physiology</topic><topic>Medical sciences</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Metabolic Diseases</topic><topic>Metabolism</topic><topic>Muscle cells</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Muscle, Skeletal - virology</topic><topic>Musculoskeletal system</topic><topic>Obesity</topic><topic>Obesity - genetics</topic><topic>Obesity - metabolism</topic><topic>Obesity - virology</topic><topic>original-article</topic><topic>Oxidation</topic><topic>Oxidation-Reduction</topic><topic>Physiological aspects</topic><topic>Protein expression</topic><topic>protein synthesis</topic><topic>Proteins</topic><topic>Proteins - genetics</topic><topic>Proteins - metabolism</topic><topic>Public Health</topic><topic>Risk factors</topic><topic>Signal Transduction</topic><topic>skeletal muscle</topic><topic>Triglycerides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Z.Q</creatorcontrib><creatorcontrib>Yu, Y</creatorcontrib><creatorcontrib>Zhang, X.H</creatorcontrib><creatorcontrib>Floyd, E.Z</creatorcontrib><creatorcontrib>Cefalu, W.T</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Psychology</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International Journal of Obesity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Z.Q</au><au>Yu, Y</au><au>Zhang, X.H</au><au>Floyd, E.Z</au><au>Cefalu, W.T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human adenovirus 36 decreases fatty acid oxidation and increases de novo lipogenesis in primary cultured human skeletal muscle cells by promoting Cidec/FSP27 expression</atitle><jtitle>International Journal of Obesity</jtitle><stitle>Int J Obes</stitle><addtitle>Int J Obes (Lond)</addtitle><date>2010-09-01</date><risdate>2010</risdate><volume>34</volume><issue>9</issue><spage>1355</spage><epage>1364</epage><pages>1355-1364</pages><issn>0307-0565</issn><eissn>1476-5497</eissn><coden>IJOBDP</coden><abstract>Background: It has been well documented that human adenovirus type 36 (Ad-36) is associated with obesity. However, the underlying molecular mechanism of Ad-36 inducing obesity remains unknown. We sought to investigate the effect of Ad-36 infection on Cidec, AMPK pathway and lipid metabolism in primary cultured human skeletal muscle cells. Methods: Cidec/fat-specific protein 27 (FSP27), fatty acid oxidation, AMPK signaling and the abundance of proteins involved in lipid synthesis were determined in muscle cells infected with various doses (1.9–7.6 MOI) of Ad-36 and non-lipogenic adenovirus type 2 (Ad-2) as a negative control as well as an uninfected control. Cidec/FSP27 siRNA transfection was performed in Ad-36-infected muscle cells. Results: Our data show that Ad-36 significantly reduced fatty acid oxidation in a dose-dependent manner (all P values are <0.01), but Ad-2 did not affect fatty acid oxidation. Ad-36 substantially increased Cidec/FSP27, ACC, sterol regulatory element-binding protein 1c (SREBP-1c), SREBP-2 and 3-hydroxy-3-methylglutaryl-CoA reductase protein abundance, but significantly reduced AMPK activity, mitochondrial mass and uncoupling protein 3 (UCP3) abundance in comparison with control cells (all P values are <0.01). Oil Red O staining revealed that there was substantial fat accumulation in the Ad-36-infected muscle cells. Furthermore, Cidec/FSP27 siRNA transfection significantly reduced FSP27 expression and partially restored AMPK signaling, increased UCP3 and decreased SERBP 1c and perilipin proteins in Ad-36-infected muscle cells. Interestingly, neither Ad-36 nor Ad-2 affected peroxisome proliferator-activated receptor γ protein expression in muscle cells. Conclusion: This study suggests that Ad-36 induced lipid droplets in the cultured skeletal muscle cells and this process may be mediated by promoting Cidec/FSP27 expression.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>20440297</pmid><doi>10.1038/ijo.2010.77</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/443/319/2723 631/80/86 692/698/1671/1668/1973 692/699/255/2514 Adenovirus diseases Adenoviruses Adenoviruses, Human - genetics Adenoviruses, Human - metabolism Adipocytes Biological and medical sciences Biological oxidation (Metabolism) Biomedical research Body fat cell culture Cells, Cultured Complications and side effects de novo lipogenesis Epidemiology fatty acid composition Fatty acids Fatty Acids - genetics Fatty Acids - metabolism Genetic aspects Glucose Health aspects Health Promotion and Disease Prevention Human adenovirus D human cell lines Humans Insulin resistance Internal Medicine Lipid Metabolism lipid peroxidation Lipids lipogenesis Lipogenesis - genetics Lipogenesis - physiology Medical sciences Medicine Medicine & Public Health Metabolic Diseases Metabolism Muscle cells Muscle, Skeletal - metabolism Muscle, Skeletal - virology Musculoskeletal system Obesity Obesity - genetics Obesity - metabolism Obesity - virology original-article Oxidation Oxidation-Reduction Physiological aspects Protein expression protein synthesis Proteins Proteins - genetics Proteins - metabolism Public Health Risk factors Signal Transduction skeletal muscle Triglycerides |
| title | Human adenovirus 36 decreases fatty acid oxidation and increases de novo lipogenesis in primary cultured human skeletal muscle cells by promoting Cidec/FSP27 expression |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T08%3A33%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Human%20adenovirus%2036%20decreases%20fatty%20acid%20oxidation%20and%20increases%20de%20novo%20lipogenesis%20in%20primary%20cultured%20human%20skeletal%20muscle%20cells%20by%20promoting%20Cidec/FSP27%20expression&rft.jtitle=International%20Journal%20of%20Obesity&rft.au=Wang,%20Z.Q&rft.date=2010-09-01&rft.volume=34&rft.issue=9&rft.spage=1355&rft.epage=1364&rft.pages=1355-1364&rft.issn=0307-0565&rft.eissn=1476-5497&rft.coden=IJOBDP&rft_id=info:doi/10.1038/ijo.2010.77&rft_dat=%3Cgale_pubme%3EA238094351%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=750310974&rft_id=info:pmid/20440297&rft_galeid=A238094351&rfr_iscdi=true |