Cancer cell-oriented migration of mesenchymal stem cells engineered with an anticancer gene (PTEN): an imaging demonstration
Mesenchymal stem cells (MSCs) have been considered to hold great potential as ideal carriers for the delivery of anticancer agents since the discovery of their tumor tropism. This study was performed to demonstrate the effects of phosphatase and tensin homolog (PTEN) engineering on MSCs' capaci...
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| Veröffentlicht in: | OncoTargets and therapy 2014-01, Vol.7, p.441-446 |
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| container_title | OncoTargets and therapy |
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| creator | Yang, Zhuo-Shun Tang, Xiang-Jun Guo, Xing-Rong Zou, Dan-Dan Sun, Xu-Yong Feng, Jing-Bo Luo, Jie Dai, Long-Jun Warnock, Garth L |
| description | Mesenchymal stem cells (MSCs) have been considered to hold great potential as ideal carriers for the delivery of anticancer agents since the discovery of their tumor tropism. This study was performed to demonstrate the effects of phosphatase and tensin homolog (PTEN) engineering on MSCs' capacity for cancer cell-oriented migration.
MSCs were engineered with a PTEN-bearing plasmid and the expression was confirmed with Western blotting. A human glioma cell line (DBTRG) was used as the target cell; DBTRG cell-oriented migration of MSCs was monitored with a micro speed photographic system.
The expression of transfected PTEN in MSCs was identified by immunoblotting analysis and confirmed with cell viability assessment of target cells. The DBTRG cell-oriented migration of PTEN-engineered MSCs was demonstrated by a real-time dynamic monitoring system, and a phagocytosis-like action of MSCs was also observed.
MSCs maintained their capacity for cancer cell-directed migration after they were engineered with anticancer genes. This study provides the first direct evidence of MSCs' tropism post-anticancer gene engineering. |
| doi_str_mv | 10.2147/OTT.S59227 |
| format | Article |
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MSCs were engineered with a PTEN-bearing plasmid and the expression was confirmed with Western blotting. A human glioma cell line (DBTRG) was used as the target cell; DBTRG cell-oriented migration of MSCs was monitored with a micro speed photographic system.
The expression of transfected PTEN in MSCs was identified by immunoblotting analysis and confirmed with cell viability assessment of target cells. The DBTRG cell-oriented migration of PTEN-engineered MSCs was demonstrated by a real-time dynamic monitoring system, and a phagocytosis-like action of MSCs was also observed.
MSCs maintained their capacity for cancer cell-directed migration after they were engineered with anticancer genes. This study provides the first direct evidence of MSCs' tropism post-anticancer gene engineering.</description><identifier>ISSN: 1178-6930</identifier><identifier>EISSN: 1178-6930</identifier><identifier>DOI: 10.2147/OTT.S59227</identifier><identifier>PMID: 24669193</identifier><language>eng</language><publisher>New Zealand: Dove Medical Press Limited</publisher><subject>cancer ; Cancer cells ; Cell migration ; gene therapy ; Genetic aspects ; Health aspects ; mesenchymal stem cells ; Oncogenes ; Original Research ; phosphatase and tensin homolog ; Stem cells</subject><ispartof>OncoTargets and therapy, 2014-01, Vol.7, p.441-446</ispartof><rights>COPYRIGHT 2014 Dove Medical Press Limited</rights><rights>2014 Yang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-dc5939c1ce02fbc66b9b6a64d7bcef0c19c47470afe8c0ab4471097d63892f213</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962313/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962313/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,3849,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24669193$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Zhuo-Shun</creatorcontrib><creatorcontrib>Tang, Xiang-Jun</creatorcontrib><creatorcontrib>Guo, Xing-Rong</creatorcontrib><creatorcontrib>Zou, Dan-Dan</creatorcontrib><creatorcontrib>Sun, Xu-Yong</creatorcontrib><creatorcontrib>Feng, Jing-Bo</creatorcontrib><creatorcontrib>Luo, Jie</creatorcontrib><creatorcontrib>Dai, Long-Jun</creatorcontrib><creatorcontrib>Warnock, Garth L</creatorcontrib><title>Cancer cell-oriented migration of mesenchymal stem cells engineered with an anticancer gene (PTEN): an imaging demonstration</title><title>OncoTargets and therapy</title><addtitle>Onco Targets Ther</addtitle><description>Mesenchymal stem cells (MSCs) have been considered to hold great potential as ideal carriers for the delivery of anticancer agents since the discovery of their tumor tropism. This study was performed to demonstrate the effects of phosphatase and tensin homolog (PTEN) engineering on MSCs' capacity for cancer cell-oriented migration.
MSCs were engineered with a PTEN-bearing plasmid and the expression was confirmed with Western blotting. A human glioma cell line (DBTRG) was used as the target cell; DBTRG cell-oriented migration of MSCs was monitored with a micro speed photographic system.
The expression of transfected PTEN in MSCs was identified by immunoblotting analysis and confirmed with cell viability assessment of target cells. The DBTRG cell-oriented migration of PTEN-engineered MSCs was demonstrated by a real-time dynamic monitoring system, and a phagocytosis-like action of MSCs was also observed.
MSCs maintained their capacity for cancer cell-directed migration after they were engineered with anticancer genes. This study provides the first direct evidence of MSCs' tropism post-anticancer gene engineering.</description><subject>cancer</subject><subject>Cancer cells</subject><subject>Cell migration</subject><subject>gene therapy</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>mesenchymal stem cells</subject><subject>Oncogenes</subject><subject>Original Research</subject><subject>phosphatase and tensin homolog</subject><subject>Stem cells</subject><issn>1178-6930</issn><issn>1178-6930</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNptkm2L1DAQx4Mo3oO-8QNIQZBT6JqHNml8IRzL-QCHJ7i-Dmk6bSNtspd0Tw7uw5va89wFSSBh5jf_zEwGoRcErygpxLurzWb1vZSUikfomBBR5Vwy_HjvfoROYvyJMecVLZ6iI1pwLolkx-hurZ2BkBkYhtwHC26CJhttF_Rkvct8m40QwZn-dtRDFicY_7AxA9dZBxAS_stOfaZd2pM1i14HDrKzb5uLr2_ezy476oR3WQOjd3Fa1J-hJ60eIjy_P0_Rj48Xm_Xn_PLq05f1-WVuSiKmvDGlZNIQA5i2teG8ljXXvGhEbaDFhkhTiEJg3UJlsK6LQhAsRcNZJWlLCTtFHxbd7a4eoTGpyKAHtQ0pq3CrvLbq0ONsrzp_o5jklBGWBPAi0Pgb2AaI8SD4n9X4URFOaJVCzu7fDP56B3FSo41z57QDv4uKlAQLLCtRJvTVgnZ6AGVd61MSZsbVeUGSHEu_lqjVf6i0Ukut8Q5am-wHAa_3AnrQw9RHP-zmzsdD8O0CmuBjDNA-FEewmidMpQlTy4Ql-OV-Lx_QvyPFfgMBCc1B</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Yang, Zhuo-Shun</creator><creator>Tang, Xiang-Jun</creator><creator>Guo, Xing-Rong</creator><creator>Zou, Dan-Dan</creator><creator>Sun, Xu-Yong</creator><creator>Feng, Jing-Bo</creator><creator>Luo, Jie</creator><creator>Dai, Long-Jun</creator><creator>Warnock, Garth L</creator><general>Dove Medical Press Limited</general><general>Dove Press</general><general>Dove Medical Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140101</creationdate><title>Cancer cell-oriented migration of mesenchymal stem cells engineered with an anticancer gene (PTEN): an imaging demonstration</title><author>Yang, Zhuo-Shun ; Tang, Xiang-Jun ; Guo, Xing-Rong ; Zou, Dan-Dan ; Sun, Xu-Yong ; Feng, Jing-Bo ; Luo, Jie ; Dai, Long-Jun ; Warnock, Garth L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c517t-dc5939c1ce02fbc66b9b6a64d7bcef0c19c47470afe8c0ab4471097d63892f213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>cancer</topic><topic>Cancer cells</topic><topic>Cell migration</topic><topic>gene therapy</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>mesenchymal stem cells</topic><topic>Oncogenes</topic><topic>Original Research</topic><topic>phosphatase and tensin homolog</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Zhuo-Shun</creatorcontrib><creatorcontrib>Tang, Xiang-Jun</creatorcontrib><creatorcontrib>Guo, Xing-Rong</creatorcontrib><creatorcontrib>Zou, Dan-Dan</creatorcontrib><creatorcontrib>Sun, Xu-Yong</creatorcontrib><creatorcontrib>Feng, Jing-Bo</creatorcontrib><creatorcontrib>Luo, Jie</creatorcontrib><creatorcontrib>Dai, Long-Jun</creatorcontrib><creatorcontrib>Warnock, Garth L</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>OncoTargets and therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Zhuo-Shun</au><au>Tang, Xiang-Jun</au><au>Guo, Xing-Rong</au><au>Zou, Dan-Dan</au><au>Sun, Xu-Yong</au><au>Feng, Jing-Bo</au><au>Luo, Jie</au><au>Dai, Long-Jun</au><au>Warnock, Garth L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cancer cell-oriented migration of mesenchymal stem cells engineered with an anticancer gene (PTEN): an imaging demonstration</atitle><jtitle>OncoTargets and therapy</jtitle><addtitle>Onco Targets Ther</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>7</volume><spage>441</spage><epage>446</epage><pages>441-446</pages><issn>1178-6930</issn><eissn>1178-6930</eissn><abstract>Mesenchymal stem cells (MSCs) have been considered to hold great potential as ideal carriers for the delivery of anticancer agents since the discovery of their tumor tropism. This study was performed to demonstrate the effects of phosphatase and tensin homolog (PTEN) engineering on MSCs' capacity for cancer cell-oriented migration.
MSCs were engineered with a PTEN-bearing plasmid and the expression was confirmed with Western blotting. A human glioma cell line (DBTRG) was used as the target cell; DBTRG cell-oriented migration of MSCs was monitored with a micro speed photographic system.
The expression of transfected PTEN in MSCs was identified by immunoblotting analysis and confirmed with cell viability assessment of target cells. The DBTRG cell-oriented migration of PTEN-engineered MSCs was demonstrated by a real-time dynamic monitoring system, and a phagocytosis-like action of MSCs was also observed.
MSCs maintained their capacity for cancer cell-directed migration after they were engineered with anticancer genes. This study provides the first direct evidence of MSCs' tropism post-anticancer gene engineering.</abstract><cop>New Zealand</cop><pub>Dove Medical Press Limited</pub><pmid>24669193</pmid><doi>10.2147/OTT.S59227</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Taylor & Francis Open Access; DOVE Medical Press Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | cancer Cancer cells Cell migration gene therapy Genetic aspects Health aspects mesenchymal stem cells Oncogenes Original Research phosphatase and tensin homolog Stem cells |
| title | Cancer cell-oriented migration of mesenchymal stem cells engineered with an anticancer gene (PTEN): an imaging demonstration |
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