Mice devoid of interferon regulatory factor 1 (IRF‐1) show normal expression of type I interferon genes
The transcription factor interferon regulatory factor 1 (IRF‐1) binds tightly to the interferon (IFN)‐beta promoter and has been implicated in the induction of type I IFNs. We generated mice devoid of functional IRF‐1 by targeted gene disruption. As reported by others, IRF‐1‐deficient mice showed a...
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| Veröffentlicht in: | The EMBO journal 1994-10, Vol.13 (20), p.4798-4806 |
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| container_title | The EMBO journal |
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| creator | Reis, L.F. Ruffner, H. Stark, G. Aguet, M. Weissmann, C. |
| description | The transcription factor interferon regulatory factor 1 (IRF‐1) binds tightly to the interferon (IFN)‐beta promoter and has been implicated in the induction of type I IFNs. We generated mice devoid of functional IRF‐1 by targeted gene disruption. As reported by others, IRF‐1‐deficient mice showed a discrete phenotype: the CD4/CD8 ratio was increased and IFN‐gamma‐induced levels of macrophage iNO synthase mRNA were strongly diminished. However, type I IFN induction in vivo by virus or double‐stranded RNA was unimpaired, as evidenced by serum IFN titers and IFN mRNA levels in spleen, liver and lung. There was also no impairment in the response of type I IFN‐inducible genes. Therefore, IRF‐1 is not essential for these processes in vivo. |
| doi_str_mv | 10.1002/j.1460-2075.1994.tb06805.x |
| format | Article |
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We generated mice devoid of functional IRF‐1 by targeted gene disruption. As reported by others, IRF‐1‐deficient mice showed a discrete phenotype: the CD4/CD8 ratio was increased and IFN‐gamma‐induced levels of macrophage iNO synthase mRNA were strongly diminished. However, type I IFN induction in vivo by virus or double‐stranded RNA was unimpaired, as evidenced by serum IFN titers and IFN mRNA levels in spleen, liver and lung. There was also no impairment in the response of type I IFN‐inducible genes. 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We generated mice devoid of functional IRF‐1 by targeted gene disruption. As reported by others, IRF‐1‐deficient mice showed a discrete phenotype: the CD4/CD8 ratio was increased and IFN‐gamma‐induced levels of macrophage iNO synthase mRNA were strongly diminished. However, type I IFN induction in vivo by virus or double‐stranded RNA was unimpaired, as evidenced by serum IFN titers and IFN mRNA levels in spleen, liver and lung. There was also no impairment in the response of type I IFN‐inducible genes. Therefore, IRF‐1 is not essential for these processes in vivo.</description><subject>Animals</subject><subject>Cell Line</subject><subject>DNA-Binding Proteins - physiology</subject><subject>Female</subject><subject>Gene Expression Regulation - physiology</subject><subject>Interferon Regulatory Factor-1</subject><subject>Interferon Type I - genetics</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred ICR</subject><subject>Molecular Sequence Data</subject><subject>Phosphoproteins - physiology</subject><subject>Transcription Factors - physiology</subject><issn>0261-4189</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkctOGzEUhq2qiKa0j1DJYlG1ixlsz_gySCwoghIEqlS1a8vjORMcTcbBnkCy6yP0GXkSnCaK6AqxOkf6Lzr2h9AhJTklhB1Nc1oKkjEieU6rqsyHmghFeL58g0Y76S0aESZoVlJVvUPvY5wSQriSdB_ty4pLUqoRcjfOAm7g3rsG-xa7foDQQvA9DjBZdGbwYYVbY9PEFH8Z_7x4_POXfsXx1j_g3oeZ6TAs5wFidCmUKobVHPD4edMEeogf0F5ruggft_MA_b44_3V2mV3_-D4-O73OLJecZ00hGYGirU3VNI3ipG4M1EpIpZjkilSq4iURrQRmlbBMlYLLknEqKZPGFsUBOtn0zhf1DBoL_RBMp-fBzUxYaW-c_l_p3a2e-HtdpGKqUv7zNh_83QLioGcuWug604NfRC3TTwsl2YtGKgQvilIm4_HGaIOPMUC7O4YSvQaqp3pNTa-p6TVQvQWqlyn86flzdtEtwaSfbvQH18HqFc36_Obb1b-9eAKhObLE</recordid><startdate>19941017</startdate><enddate>19941017</enddate><creator>Reis, L.F.</creator><creator>Ruffner, H.</creator><creator>Stark, G.</creator><creator>Aguet, M.</creator><creator>Weissmann, C.</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19941017</creationdate><title>Mice devoid of interferon regulatory factor 1 (IRF‐1) show normal expression of type I interferon genes</title><author>Reis, L.F. ; Ruffner, H. ; Stark, G. ; Aguet, M. ; Weissmann, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5755-d3720e3fba9ddd850bdaeb86788275809895406f7e2c86c28465742517127ac33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Cell Line</topic><topic>DNA-Binding Proteins - physiology</topic><topic>Female</topic><topic>Gene Expression Regulation - physiology</topic><topic>Interferon Regulatory Factor-1</topic><topic>Interferon Type I - genetics</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred ICR</topic><topic>Molecular Sequence Data</topic><topic>Phosphoproteins - physiology</topic><topic>Transcription Factors - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reis, L.F.</creatorcontrib><creatorcontrib>Ruffner, H.</creatorcontrib><creatorcontrib>Stark, G.</creatorcontrib><creatorcontrib>Aguet, M.</creatorcontrib><creatorcontrib>Weissmann, C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reis, L.F.</au><au>Ruffner, H.</au><au>Stark, G.</au><au>Aguet, M.</au><au>Weissmann, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mice devoid of interferon regulatory factor 1 (IRF‐1) show normal expression of type I interferon genes</atitle><jtitle>The EMBO journal</jtitle><addtitle>EMBO J</addtitle><date>1994-10-17</date><risdate>1994</risdate><volume>13</volume><issue>20</issue><spage>4798</spage><epage>4806</epage><pages>4798-4806</pages><issn>0261-4189</issn><eissn>1460-2075</eissn><abstract>The transcription factor interferon regulatory factor 1 (IRF‐1) binds tightly to the interferon (IFN)‐beta promoter and has been implicated in the induction of type I IFNs. We generated mice devoid of functional IRF‐1 by targeted gene disruption. As reported by others, IRF‐1‐deficient mice showed a discrete phenotype: the CD4/CD8 ratio was increased and IFN‐gamma‐induced levels of macrophage iNO synthase mRNA were strongly diminished. However, type I IFN induction in vivo by virus or double‐stranded RNA was unimpaired, as evidenced by serum IFN titers and IFN mRNA levels in spleen, liver and lung. There was also no impairment in the response of type I IFN‐inducible genes. Therefore, IRF‐1 is not essential for these processes in vivo.</abstract><cop>England</cop><pmid>7957048</pmid><doi>10.1002/j.1460-2075.1994.tb06805.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0261-4189 |
| ispartof | The EMBO journal, 1994-10, Vol.13 (20), p.4798-4806 |
| issn | 0261-4189 1460-2075 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Cell Line DNA-Binding Proteins - physiology Female Gene Expression Regulation - physiology Interferon Regulatory Factor-1 Interferon Type I - genetics Male Mice Mice, Inbred C57BL Mice, Inbred ICR Molecular Sequence Data Phosphoproteins - physiology Transcription Factors - physiology |
| title | Mice devoid of interferon regulatory factor 1 (IRF‐1) show normal expression of type I interferon genes |
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