Three ARS elements contribute to the ura4 replication origin region in the fission yeast, Schizosaccharomyces pombe
The ura4 replication origin region, which is located near the ura4 gene on chromosome III of the fission yeast, Schizosaccharomyces pombe, contains multiple initiation sites. We have used 2D gel electrophoretic replicon mapping methods to study the distribution of these initiation sites, and have fo...
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| Veröffentlicht in: | The EMBO journal 1994-08, Vol.13 (15), p.3638-3647 |
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| creator | Dubey, D.D Zhu, J Carlson, D.L Sharma, K Huberman, J.A |
| description | The ura4 replication origin region, which is located near the ura4 gene on chromosome III of the fission yeast, Schizosaccharomyces pombe, contains multiple initiation sites. We have used 2D gel electrophoretic replicon mapping methods to study the distribution of these initiation sites, and have found that they are concentrated near three ARS elements (stretches of DNA which permit autonomous plasmid replication). To detemine the roles of these ARS elements in the function of the ura4 origin region, we deleted either one or two of them from the chromosome and then assessed the consequences of the deletions by 2D gel electrophoresis. The results suggest that each of the three ARS elements is responsible for the initiation events in its vicinity and that the ARS elements interfere with each other in a hierarchical fashion. It is possible that the large initiation zones of animal cells are similarly composed of multiple mutally inteferring origins. |
| doi_str_mv | 10.1002/j.1460-2075.1994.tb06671.x |
| format | Article |
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We have used 2D gel electrophoretic replicon mapping methods to study the distribution of these initiation sites, and have found that they are concentrated near three ARS elements (stretches of DNA which permit autonomous plasmid replication). To detemine the roles of these ARS elements in the function of the ura4 origin region, we deleted either one or two of them from the chromosome and then assessed the consequences of the deletions by 2D gel electrophoresis. The results suggest that each of the three ARS elements is responsible for the initiation events in its vicinity and that the ARS elements interfere with each other in a hierarchical fashion. It is possible that the large initiation zones of animal cells are similarly composed of multiple mutally inteferring origins.</description><identifier>ISSN: 0261-4189</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1002/j.1460-2075.1994.tb06671.x</identifier><identifier>PMID: 8062838</identifier><identifier>CODEN: EMJODG</identifier><language>eng</language><publisher>London: Nature Publishing Group</publisher><subject>autonomously replicating sequence ; Biological and medical sciences ; Chromosome Mapping ; Chromosomes, Fungal ; DNA ; DNA replication ; DNA Replication - genetics ; DNA Replication - physiology ; DNA, Fungal - analysis ; DNA, Fungal - chemistry ; Electrophoresis, Gel, Two-Dimensional ; Fundamental and applied biological sciences. Psychology ; Genes, Fungal - genetics ; Molecular and cellular biology ; Molecular genetics ; Nucleic Acid Conformation ; nucleotide sequences ; Regulatory Sequences, Nucleic Acid - genetics ; Replication ; Saccharomycetales ; Schizosaccharomyces - genetics ; Sequence Deletion - physiology ; structural genes ; ura4 gene</subject><ispartof>The EMBO journal, 1994-08, Vol.13 (15), p.3638-3647</ispartof><rights>1994 European Molecular Biology Organization</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4461-ab67e697aa9f9949eb844885d08773ec3d43ed135df1a9f0cf4cf87c405640f13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC395269/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC395269/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4234845$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8062838$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dubey, D.D</creatorcontrib><creatorcontrib>Zhu, J</creatorcontrib><creatorcontrib>Carlson, D.L</creatorcontrib><creatorcontrib>Sharma, K</creatorcontrib><creatorcontrib>Huberman, J.A</creatorcontrib><title>Three ARS elements contribute to the ura4 replication origin region in the fission yeast, Schizosaccharomyces pombe</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><description>The ura4 replication origin region, which is located near the ura4 gene on chromosome III of the fission yeast, Schizosaccharomyces pombe, contains multiple initiation sites. We have used 2D gel electrophoretic replicon mapping methods to study the distribution of these initiation sites, and have found that they are concentrated near three ARS elements (stretches of DNA which permit autonomous plasmid replication). To detemine the roles of these ARS elements in the function of the ura4 origin region, we deleted either one or two of them from the chromosome and then assessed the consequences of the deletions by 2D gel electrophoresis. The results suggest that each of the three ARS elements is responsible for the initiation events in its vicinity and that the ARS elements interfere with each other in a hierarchical fashion. It is possible that the large initiation zones of animal cells are similarly composed of multiple mutally inteferring origins.</description><subject>autonomously replicating sequence</subject><subject>Biological and medical sciences</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Fungal</subject><subject>DNA</subject><subject>DNA replication</subject><subject>DNA Replication - genetics</subject><subject>DNA Replication - physiology</subject><subject>DNA, Fungal - analysis</subject><subject>DNA, Fungal - chemistry</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Fungal - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Nucleic Acid Conformation</subject><subject>nucleotide sequences</subject><subject>Regulatory Sequences, Nucleic Acid - genetics</subject><subject>Replication</subject><subject>Saccharomycetales</subject><subject>Schizosaccharomyces - genetics</subject><subject>Sequence Deletion - physiology</subject><subject>structural genes</subject><subject>ura4 gene</subject><issn>0261-4189</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVUUuP0zAQthBoKQs_AWEhjjSMYydxkDiU1fLSIiS6e7YcZ9y4SuLKTmHLr8ehVQVHTp7x95gXIS8ZZAwgf7PNmChhmUNVZKyuRTY1UJYVy-4fkMUZekgWkJdsKZisH5MnMW4BoJAVuyAXEspccrkg8bYLiHT1fU2xxwHHKVLjxym4Zj8hnTydOqT7oAUNuOud0ZPzI_XBbdyYvjZzlqKZZV2Mc3pAHafXdG0698tHbUyngx8OBiPd-aHBp-SR1X3EZ6f3ktx9uL69-rS8-fbx89XqZmmESH3rpqywrCuta5uGrLGRQkhZtCCriqPhreDYMl60liUKGCuMlZURUJQCLOOX5N3Rd7dvBmxNGi7oXu2CG3Q4KK-d-hcZXac2_ofidZGXddK_PepN8DEGtGcpAzUfQm3VvG01b1vNh1CnQ6j7JH7-d_Gz9LT5hL864Toa3dugR-PimSZyLqQoEm11pP10PR7-owF1_fX9lz9x8nhx9LDaK70JqczdOgfGgYlaFCD4b2UosoA</recordid><startdate>199408</startdate><enddate>199408</enddate><creator>Dubey, D.D</creator><creator>Zhu, J</creator><creator>Carlson, D.L</creator><creator>Sharma, K</creator><creator>Huberman, J.A</creator><general>Nature Publishing Group</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>199408</creationdate><title>Three ARS elements contribute to the ura4 replication origin region in the fission yeast, Schizosaccharomyces pombe</title><author>Dubey, D.D ; Zhu, J ; Carlson, D.L ; Sharma, K ; Huberman, J.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4461-ab67e697aa9f9949eb844885d08773ec3d43ed135df1a9f0cf4cf87c405640f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>autonomously replicating sequence</topic><topic>Biological and medical sciences</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Fungal</topic><topic>DNA</topic><topic>DNA replication</topic><topic>DNA Replication - genetics</topic><topic>DNA Replication - physiology</topic><topic>DNA, Fungal - analysis</topic><topic>DNA, Fungal - chemistry</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Fungal - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Nucleic Acid Conformation</topic><topic>nucleotide sequences</topic><topic>Regulatory Sequences, Nucleic Acid - genetics</topic><topic>Replication</topic><topic>Saccharomycetales</topic><topic>Schizosaccharomyces - genetics</topic><topic>Sequence Deletion - physiology</topic><topic>structural genes</topic><topic>ura4 gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dubey, D.D</creatorcontrib><creatorcontrib>Zhu, J</creatorcontrib><creatorcontrib>Carlson, D.L</creatorcontrib><creatorcontrib>Sharma, K</creatorcontrib><creatorcontrib>Huberman, J.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dubey, D.D</au><au>Zhu, J</au><au>Carlson, D.L</au><au>Sharma, K</au><au>Huberman, J.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Three ARS elements contribute to the ura4 replication origin region in the fission yeast, Schizosaccharomyces pombe</atitle><jtitle>The EMBO journal</jtitle><addtitle>EMBO J</addtitle><date>1994-08</date><risdate>1994</risdate><volume>13</volume><issue>15</issue><spage>3638</spage><epage>3647</epage><pages>3638-3647</pages><issn>0261-4189</issn><eissn>1460-2075</eissn><coden>EMJODG</coden><abstract>The ura4 replication origin region, which is located near the ura4 gene on chromosome III of the fission yeast, Schizosaccharomyces pombe, contains multiple initiation sites. We have used 2D gel electrophoretic replicon mapping methods to study the distribution of these initiation sites, and have found that they are concentrated near three ARS elements (stretches of DNA which permit autonomous plasmid replication). To detemine the roles of these ARS elements in the function of the ura4 origin region, we deleted either one or two of them from the chromosome and then assessed the consequences of the deletions by 2D gel electrophoresis. The results suggest that each of the three ARS elements is responsible for the initiation events in its vicinity and that the ARS elements interfere with each other in a hierarchical fashion. It is possible that the large initiation zones of animal cells are similarly composed of multiple mutally inteferring origins.</abstract><cop>London</cop><pub>Nature Publishing Group</pub><pmid>8062838</pmid><doi>10.1002/j.1460-2075.1994.tb06671.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | autonomously replicating sequence Biological and medical sciences Chromosome Mapping Chromosomes, Fungal DNA DNA replication DNA Replication - genetics DNA Replication - physiology DNA, Fungal - analysis DNA, Fungal - chemistry Electrophoresis, Gel, Two-Dimensional Fundamental and applied biological sciences. Psychology Genes, Fungal - genetics Molecular and cellular biology Molecular genetics Nucleic Acid Conformation nucleotide sequences Regulatory Sequences, Nucleic Acid - genetics Replication Saccharomycetales Schizosaccharomyces - genetics Sequence Deletion - physiology structural genes ura4 gene |
| title | Three ARS elements contribute to the ura4 replication origin region in the fission yeast, Schizosaccharomyces pombe |
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