Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening
Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current tran...
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| Veröffentlicht in: | Korean journal of parasitology 2014, Hosts and Diseases, 52(1), , pp.21-26 |
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| creator | Fu, Yan Lan, Jingchao Wu, Xuhang Yang, Deying Zhang, Zhihe Nie, Huaming Hou, Rong Zhang, Runhui Zheng, Wanpeng Xie, Yue Yan, Ning Yang, Zhi Wang, Chengdong Luo, Li Liu, Li Gu, Xiaobin Wang, Shuxian Peng, Xuerong Yang, Guangyou |
| description | Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose. |
| doi_str_mv | 10.3347/kjp.2014.52.1.21 |
| format | Article |
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More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.</description><identifier>ISSN: 0023-4001</identifier><identifier>ISSN: 1738-0006</identifier><identifier>ISSN: 2982-5164</identifier><identifier>EISSN: 1738-0006</identifier><identifier>EISSN: 2982-6799</identifier><identifier>DOI: 10.3347/kjp.2014.52.1.21</identifier><identifier>PMID: 24623877</identifier><language>eng</language><publisher>Korea (South): 대한기생충학열대의학회</publisher><subject>Animal Structures - chemistry ; Animals ; Antibodies, Helminth - blood ; Antigens, Helminth - chemistry ; Antigens, Helminth - genetics ; Antigens, Helminth - immunology ; Antigens, Helminth - isolation & purification ; Biomarkers, Tumor - chemistry ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - immunology ; Biomarkers, Tumor - isolation & purification ; Cloning, Molecular ; Dirofilaria immitis - chemistry ; Dirofilaria immitis - genetics ; Dirofilaria immitis - immunology ; Disease Models, Animal ; Dogs ; Escherichia coli - genetics ; Gene Expression ; Molecular Sequence Data ; Molecular Weight ; Original ; Recombinant Fusion Proteins - chemistry ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - immunology ; Recombinant Fusion Proteins - isolation & purification ; Sequence Analysis, DNA ; 예방의학</subject><ispartof>Parasites, 2014, Hosts and Diseases, 52(1), , pp.21-26</ispartof><rights>2014, Korean Society for Parasitology and Tropical Medicine 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-cdbf5b1c0491c073270d044ca661e1b0d0c7a0644df73cbcacdbbec15052e07a3</citedby><cites>FETCH-LOGICAL-c460t-cdbf5b1c0491c073270d044ca661e1b0d0c7a0644df73cbcacdbbec15052e07a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3948989/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3948989/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24623877$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART002156869$$DAccess content in National Research Foundation of Korea (NRF)$$Hfree_for_read</backlink></links><search><creatorcontrib>Fu, Yan</creatorcontrib><creatorcontrib>Lan, Jingchao</creatorcontrib><creatorcontrib>Wu, Xuhang</creatorcontrib><creatorcontrib>Yang, Deying</creatorcontrib><creatorcontrib>Zhang, Zhihe</creatorcontrib><creatorcontrib>Nie, Huaming</creatorcontrib><creatorcontrib>Hou, Rong</creatorcontrib><creatorcontrib>Zhang, Runhui</creatorcontrib><creatorcontrib>Zheng, Wanpeng</creatorcontrib><creatorcontrib>Xie, Yue</creatorcontrib><creatorcontrib>Yan, Ning</creatorcontrib><creatorcontrib>Yang, Zhi</creatorcontrib><creatorcontrib>Wang, Chengdong</creatorcontrib><creatorcontrib>Luo, Li</creatorcontrib><creatorcontrib>Liu, Li</creatorcontrib><creatorcontrib>Gu, Xiaobin</creatorcontrib><creatorcontrib>Wang, Shuxian</creatorcontrib><creatorcontrib>Peng, Xuerong</creatorcontrib><creatorcontrib>Yang, Guangyou</creatorcontrib><title>Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening</title><title>Korean journal of parasitology</title><addtitle>Korean J Parasitol</addtitle><description>Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.</description><subject>Animal Structures - chemistry</subject><subject>Animals</subject><subject>Antibodies, Helminth - blood</subject><subject>Antigens, Helminth - chemistry</subject><subject>Antigens, Helminth - genetics</subject><subject>Antigens, Helminth - immunology</subject><subject>Antigens, Helminth - isolation & purification</subject><subject>Biomarkers, Tumor - chemistry</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - immunology</subject><subject>Biomarkers, Tumor - isolation & purification</subject><subject>Cloning, Molecular</subject><subject>Dirofilaria immitis - chemistry</subject><subject>Dirofilaria immitis - genetics</subject><subject>Dirofilaria immitis - immunology</subject><subject>Disease Models, Animal</subject><subject>Dogs</subject><subject>Escherichia coli - genetics</subject><subject>Gene Expression</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>Original</subject><subject>Recombinant Fusion Proteins - chemistry</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>Recombinant Fusion Proteins - isolation & purification</subject><subject>Sequence Analysis, DNA</subject><subject>예방의학</subject><issn>0023-4001</issn><issn>1738-0006</issn><issn>2982-5164</issn><issn>1738-0006</issn><issn>2982-6799</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVks9v0zAUxyMEYt3gzgn5grQdEp5_JE4vSFNXyqSJTRDOluM4w6tjd3aCqLQ_HnctFVz8ZL3P9-tnf51l7zAUlDL-cf2wKQhgVpSkwAXBL7IZ5rTOAaB6mc0ACM0ZAD7JTmN8AKCk5Ph1dkJYRWjN-Sx7Wv7eBB2j8Q75HjVBumjlmLbS2i1aeDcGb63uUDMNPqC74EdtHDpvFs3dBVppp3e6KxN8b6wMRiIzDGY0Ea0m0yVdu927qmA2ox-MQt9V0NoZd_8me9VLG_XbQz3LfnxeNosv-c3t6npxeZMrVsGYq67tyxYrYPO0cEo4dMCYklWFNW7TRnEJFWNdz6lqlUyCVitcQkk0cEnPsou9rwu9WCsjvDTP9d6LdRCX35prgUlV13ie2E97djO1g-6UTg8grdgEM8iwfVb-33HmZ_L5Jeic1fN6Z3B-MAj-cdJxFIOJSlsrnfZTFGkszhNblgmFPaqCjzHo_ngMBrELWKSAxS5gURKBBcFJ8v7f8Y6Cv4km4MPhrlNq6c7II_P19mqZfgSHuuT0D46DsQ0</recordid><startdate>20140201</startdate><enddate>20140201</enddate><creator>Fu, Yan</creator><creator>Lan, Jingchao</creator><creator>Wu, Xuhang</creator><creator>Yang, Deying</creator><creator>Zhang, Zhihe</creator><creator>Nie, Huaming</creator><creator>Hou, Rong</creator><creator>Zhang, Runhui</creator><creator>Zheng, Wanpeng</creator><creator>Xie, Yue</creator><creator>Yan, Ning</creator><creator>Yang, Zhi</creator><creator>Wang, Chengdong</creator><creator>Luo, Li</creator><creator>Liu, Li</creator><creator>Gu, Xiaobin</creator><creator>Wang, Shuxian</creator><creator>Peng, Xuerong</creator><creator>Yang, Guangyou</creator><general>대한기생충학열대의학회</general><general>The Korean Society for Parasitology and Tropical Medicine</general><general>대한기생충학ㆍ열대의학회</general><scope>DBRKI</scope><scope>TDB</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>ACYCR</scope></search><sort><creationdate>20140201</creationdate><title>Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening</title><author>Fu, Yan ; Lan, Jingchao ; Wu, Xuhang ; Yang, Deying ; Zhang, Zhihe ; Nie, Huaming ; Hou, Rong ; Zhang, Runhui ; Zheng, Wanpeng ; Xie, Yue ; Yan, Ning ; Yang, Zhi ; Wang, Chengdong ; Luo, Li ; Liu, Li ; Gu, Xiaobin ; Wang, Shuxian ; Peng, Xuerong ; Yang, Guangyou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-cdbf5b1c0491c073270d044ca661e1b0d0c7a0644df73cbcacdbbec15052e07a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animal Structures - chemistry</topic><topic>Animals</topic><topic>Antibodies, Helminth - blood</topic><topic>Antigens, Helminth - chemistry</topic><topic>Antigens, Helminth - genetics</topic><topic>Antigens, Helminth - immunology</topic><topic>Antigens, Helminth - isolation & purification</topic><topic>Biomarkers, Tumor - chemistry</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - immunology</topic><topic>Biomarkers, Tumor - isolation & purification</topic><topic>Cloning, Molecular</topic><topic>Dirofilaria immitis - chemistry</topic><topic>Dirofilaria immitis - genetics</topic><topic>Dirofilaria immitis - immunology</topic><topic>Disease Models, Animal</topic><topic>Dogs</topic><topic>Escherichia coli - genetics</topic><topic>Gene Expression</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>Original</topic><topic>Recombinant Fusion Proteins - chemistry</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>Recombinant Fusion Proteins - isolation & purification</topic><topic>Sequence Analysis, DNA</topic><topic>예방의학</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fu, Yan</creatorcontrib><creatorcontrib>Lan, Jingchao</creatorcontrib><creatorcontrib>Wu, Xuhang</creatorcontrib><creatorcontrib>Yang, Deying</creatorcontrib><creatorcontrib>Zhang, Zhihe</creatorcontrib><creatorcontrib>Nie, Huaming</creatorcontrib><creatorcontrib>Hou, Rong</creatorcontrib><creatorcontrib>Zhang, Runhui</creatorcontrib><creatorcontrib>Zheng, Wanpeng</creatorcontrib><creatorcontrib>Xie, Yue</creatorcontrib><creatorcontrib>Yan, Ning</creatorcontrib><creatorcontrib>Yang, Zhi</creatorcontrib><creatorcontrib>Wang, Chengdong</creatorcontrib><creatorcontrib>Luo, Li</creatorcontrib><creatorcontrib>Liu, Li</creatorcontrib><creatorcontrib>Gu, Xiaobin</creatorcontrib><creatorcontrib>Wang, Shuxian</creatorcontrib><creatorcontrib>Peng, Xuerong</creatorcontrib><creatorcontrib>Yang, Guangyou</creatorcontrib><collection>DBPIA - 디비피아</collection><collection>DBPIA</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Korean Citation Index</collection><jtitle>Korean journal of parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fu, Yan</au><au>Lan, Jingchao</au><au>Wu, Xuhang</au><au>Yang, Deying</au><au>Zhang, Zhihe</au><au>Nie, Huaming</au><au>Hou, Rong</au><au>Zhang, Runhui</au><au>Zheng, Wanpeng</au><au>Xie, Yue</au><au>Yan, Ning</au><au>Yang, Zhi</au><au>Wang, Chengdong</au><au>Luo, Li</au><au>Liu, Li</au><au>Gu, Xiaobin</au><au>Wang, Shuxian</au><au>Peng, Xuerong</au><au>Yang, Guangyou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening</atitle><jtitle>Korean journal of parasitology</jtitle><addtitle>Korean J Parasitol</addtitle><date>2014-02-01</date><risdate>2014</risdate><volume>52</volume><issue>1</issue><spage>21</spage><epage>26</epage><pages>21-26</pages><issn>0023-4001</issn><issn>1738-0006</issn><issn>2982-5164</issn><eissn>1738-0006</eissn><eissn>2982-6799</eissn><abstract>Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.</abstract><cop>Korea (South)</cop><pub>대한기생충학열대의학회</pub><pmid>24623877</pmid><doi>10.3347/kjp.2014.52.1.21</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Parasites, 2014, Hosts and Diseases, 52(1), , pp.21-26 |
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| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3948989 |
| source | MEDLINE; KoreaMed Open Access; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animal Structures - chemistry Animals Antibodies, Helminth - blood Antigens, Helminth - chemistry Antigens, Helminth - genetics Antigens, Helminth - immunology Antigens, Helminth - isolation & purification Biomarkers, Tumor - chemistry Biomarkers, Tumor - genetics Biomarkers, Tumor - immunology Biomarkers, Tumor - isolation & purification Cloning, Molecular Dirofilaria immitis - chemistry Dirofilaria immitis - genetics Dirofilaria immitis - immunology Disease Models, Animal Dogs Escherichia coli - genetics Gene Expression Molecular Sequence Data Molecular Weight Original Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Recombinant Fusion Proteins - isolation & purification Sequence Analysis, DNA 예방의학 |
| title | Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening |
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