splicing factor PRP2, a putative RNA helicase, interacts directly with pre-mRNA

The RNA helicase-like splicing factor PRP2 interacts only transiently with spliceosomes. To facilitate analysis of interactions of PRP2 with spliceosomal components, PRP2 protein was stalled in splicing complexes by two different methods. A dominant negative mutant form of PRP2 protein, which associ...

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Veröffentlicht in:	The EMBO journal 1994-02, Vol.13 (4), p.888-897
Hauptverfasser:	Teigelkamp, S, McGarvey, M, Plumpton, M, Beggs, J.D
Format:	Artikel
Sprache:	eng
Schlagworte:	adenosine triphosphate Adenosine Triphosphate - metabolism adenosinetriphosphatase alternative splicing Base Sequence binding proteins Biological and medical sciences DEAD-box RNA Helicases Fundamental and applied biological sciences. Psychology Fungal Proteins - metabolism Fungal Proteins - radiation effects Hydrolysis messenger RNA Molecular and cellular biology Molecular genetics Molecular Sequence Data mutagenesis mutants precursors RNA Helicases RNA Nucleotidyltransferases - metabolism RNA Precursors - metabolism RNA Splicing RNA, Fungal - metabolism RNA, Messenger - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins spliceosomes Spliceosomes - metabolism Transcription. Transcription factor. Splicing. Rna processing Ultraviolet Rays
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