Arsenic exposure perturbs the gut microbiome and its metabolic profile in mice: an integrated metagenomics and metabolomics analysis
The human intestine is host to an enormously complex, diverse, and vast microbial community-the gut microbiota. The gut microbiome plays a profound role in metabolic processing, energy production, immune and cognitive development, epithelial homeostasis, and so forth. However, the composition and di...
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| Veröffentlicht in: | Environmental health perspectives 2014-03, Vol.122 (3), p.284-291 |
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| creator | Lu, Kun Abo, Ryan Phillip Schlieper, Katherine Ann Graffam, Michelle E Levine, Stuart Wishnok, John S Swenberg, James A Tannenbaum, Steven R Fox, James G |
| description | The human intestine is host to an enormously complex, diverse, and vast microbial community-the gut microbiota. The gut microbiome plays a profound role in metabolic processing, energy production, immune and cognitive development, epithelial homeostasis, and so forth. However, the composition and diversity of the gut microbiome can be readily affected by external factors, which raises the possibility that exposure to toxic environmental chemicals leads to gut microbiome alteration, or dysbiosis. Arsenic exposure affects large human populations worldwide and has been linked to a number of diseases, including cancer, diabetes, and cardiovascular disorders.
We investigated the impact of arsenic exposure on the gut microbiome composition and its metabolic profiles.
We used an integrated approach combining 16S rRNA gene sequencing and mass spectrometry-based metabolomics profiling to examine the functional impact of arsenic exposure on the gut microbiome.
16S rRNA gene sequencing revealed that arsenic significantly perturbed the gut microbiome composition in C57BL/6 mice after exposure to 10 ppm arsenic for 4 weeks in drinking water. Moreover, metabolomics profiling revealed a concurrent effect, with a number of gut microflora-related metabolites being perturbed in multiple biological matrices.
Arsenic exposure not only alters the gut microbiome community at the abundance level but also substantially disturbs its metabolic profiles at the function level. These findings may provide novel insights regarding perturbations of the gut microbiome and its functions as a potential new mechanism by which arsenic exposure leads to or exacerbates human diseases.
Lu K, Abo RP, Schlieper KA, Graffam ME, Levine S, Wishnok JS, Swenberg JA, Tannenbaum SR, Fox JG. 2014. Arsenic exposure perturbs the gut microbiome and its metabolic profile in mice: an integrated metagenomics and metabolomics analysis. Environ Health Perspect 122:284-291; http://dx.doi.org/10.1289/ehp.1307429. |
| doi_str_mv | 10.1289/ehp.1307429 |
| format | Article |
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We investigated the impact of arsenic exposure on the gut microbiome composition and its metabolic profiles.
We used an integrated approach combining 16S rRNA gene sequencing and mass spectrometry-based metabolomics profiling to examine the functional impact of arsenic exposure on the gut microbiome.
16S rRNA gene sequencing revealed that arsenic significantly perturbed the gut microbiome composition in C57BL/6 mice after exposure to 10 ppm arsenic for 4 weeks in drinking water. Moreover, metabolomics profiling revealed a concurrent effect, with a number of gut microflora-related metabolites being perturbed in multiple biological matrices.
Arsenic exposure not only alters the gut microbiome community at the abundance level but also substantially disturbs its metabolic profiles at the function level. These findings may provide novel insights regarding perturbations of the gut microbiome and its functions as a potential new mechanism by which arsenic exposure leads to or exacerbates human diseases.
Lu K, Abo RP, Schlieper KA, Graffam ME, Levine S, Wishnok JS, Swenberg JA, Tannenbaum SR, Fox JG. 2014. Arsenic exposure perturbs the gut microbiome and its metabolic profile in mice: an integrated metagenomics and metabolomics analysis. Environ Health Perspect 122:284-291; http://dx.doi.org/10.1289/ehp.1307429.</description><identifier>ISSN: 0091-6765</identifier><identifier>EISSN: 1552-9924</identifier><identifier>DOI: 10.1289/ehp.1307429</identifier><identifier>PMID: 24413286</identifier><language>eng</language><publisher>United States: National Institute of Environmental Health Sciences</publisher><subject>Animals ; Arsenic ; Arsenic - toxicity ; Bacteria ; Chromatography ; Chromatography, Liquid ; Diabetes ; Diseases ; DNA Barcoding, Taxonomic ; DNA repair ; Drinking water ; Environmental aspects ; Environmental health ; Experiments ; Exposure ; Female ; Gastrointestinal Tract - drug effects ; Gastrointestinal Tract - microbiology ; Gene expression ; Gene sequencing ; Genomes ; Health ; Health aspects ; Human ; Human populations ; Integrated approach ; Laboratory animals ; Mass Spectrometry ; Metabolism ; Metabolites ; Metabolome ; Metagenome - drug effects ; Mice ; Mice, Inbred C57BL ; Microbiota ; Microbiota (Symbiotic organisms) ; Microbiota - drug effects ; Molecular Sequence Data ; PCB ; Polychlorinated biphenyls ; Polycyclic aromatic hydrocarbons ; Profiling ; RNA, Ribosomal, 16S - genetics ; RNA, Ribosomal, 16S - metabolism ; Scientific imaging ; Sequence Analysis, DNA ; Signal transduction ; Software ; Specific Pathogen-Free Organisms ; Studies ; Taxonomy</subject><ispartof>Environmental health perspectives, 2014-03, Vol.122 (3), p.284-291</ispartof><rights>COPYRIGHT 2014 National Institute of Environmental Health Sciences</rights><rights>Copyright National Institute of Environmental Health Sciences Mar 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c679t-b405c16bfb931edf106d8770879eb048ca0b649bb09a1eb849146680a3003da33</citedby><cites>FETCH-LOGICAL-c679t-b405c16bfb931edf106d8770879eb048ca0b649bb09a1eb849146680a3003da33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3948040/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3948040/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24413286$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lu, Kun</creatorcontrib><creatorcontrib>Abo, Ryan Phillip</creatorcontrib><creatorcontrib>Schlieper, Katherine Ann</creatorcontrib><creatorcontrib>Graffam, Michelle E</creatorcontrib><creatorcontrib>Levine, Stuart</creatorcontrib><creatorcontrib>Wishnok, John S</creatorcontrib><creatorcontrib>Swenberg, James A</creatorcontrib><creatorcontrib>Tannenbaum, Steven R</creatorcontrib><creatorcontrib>Fox, James G</creatorcontrib><title>Arsenic exposure perturbs the gut microbiome and its metabolic profile in mice: an integrated metagenomics and metabolomics analysis</title><title>Environmental health perspectives</title><addtitle>Environ Health Perspect</addtitle><description>The human intestine is host to an enormously complex, diverse, and vast microbial community-the gut microbiota. The gut microbiome plays a profound role in metabolic processing, energy production, immune and cognitive development, epithelial homeostasis, and so forth. However, the composition and diversity of the gut microbiome can be readily affected by external factors, which raises the possibility that exposure to toxic environmental chemicals leads to gut microbiome alteration, or dysbiosis. Arsenic exposure affects large human populations worldwide and has been linked to a number of diseases, including cancer, diabetes, and cardiovascular disorders.
We investigated the impact of arsenic exposure on the gut microbiome composition and its metabolic profiles.
We used an integrated approach combining 16S rRNA gene sequencing and mass spectrometry-based metabolomics profiling to examine the functional impact of arsenic exposure on the gut microbiome.
16S rRNA gene sequencing revealed that arsenic significantly perturbed the gut microbiome composition in C57BL/6 mice after exposure to 10 ppm arsenic for 4 weeks in drinking water. Moreover, metabolomics profiling revealed a concurrent effect, with a number of gut microflora-related metabolites being perturbed in multiple biological matrices.
Arsenic exposure not only alters the gut microbiome community at the abundance level but also substantially disturbs its metabolic profiles at the function level. These findings may provide novel insights regarding perturbations of the gut microbiome and its functions as a potential new mechanism by which arsenic exposure leads to or exacerbates human diseases.
Lu K, Abo RP, Schlieper KA, Graffam ME, Levine S, Wishnok JS, Swenberg JA, Tannenbaum SR, Fox JG. 2014. Arsenic exposure perturbs the gut microbiome and its metabolic profile in mice: an integrated metagenomics and metabolomics analysis. Environ Health Perspect 122:284-291; http://dx.doi.org/10.1289/ehp.1307429.</description><subject>Animals</subject><subject>Arsenic</subject><subject>Arsenic - toxicity</subject><subject>Bacteria</subject><subject>Chromatography</subject><subject>Chromatography, Liquid</subject><subject>Diabetes</subject><subject>Diseases</subject><subject>DNA Barcoding, Taxonomic</subject><subject>DNA repair</subject><subject>Drinking water</subject><subject>Environmental aspects</subject><subject>Environmental health</subject><subject>Experiments</subject><subject>Exposure</subject><subject>Female</subject><subject>Gastrointestinal Tract - drug effects</subject><subject>Gastrointestinal Tract - microbiology</subject><subject>Gene expression</subject><subject>Gene sequencing</subject><subject>Genomes</subject><subject>Health</subject><subject>Health aspects</subject><subject>Human</subject><subject>Human populations</subject><subject>Integrated approach</subject><subject>Laboratory animals</subject><subject>Mass Spectrometry</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Metabolome</subject><subject>Metagenome - drug effects</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microbiota</subject><subject>Microbiota (Symbiotic organisms)</subject><subject>Microbiota - drug effects</subject><subject>Molecular Sequence Data</subject><subject>PCB</subject><subject>Polychlorinated biphenyls</subject><subject>Polycyclic aromatic hydrocarbons</subject><subject>Profiling</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>RNA, Ribosomal, 16S - metabolism</subject><subject>Scientific imaging</subject><subject>Sequence Analysis, DNA</subject><subject>Signal transduction</subject><subject>Software</subject><subject>Specific Pathogen-Free 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exposure perturbs the gut microbiome and its metabolic profile in mice: an integrated metagenomics and metabolomics analysis</title><author>Lu, Kun ; Abo, Ryan Phillip ; Schlieper, Katherine Ann ; Graffam, Michelle E ; Levine, Stuart ; Wishnok, John S ; Swenberg, James A ; Tannenbaum, Steven R ; Fox, James G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c679t-b405c16bfb931edf106d8770879eb048ca0b649bb09a1eb849146680a3003da33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Arsenic</topic><topic>Arsenic - toxicity</topic><topic>Bacteria</topic><topic>Chromatography</topic><topic>Chromatography, Liquid</topic><topic>Diabetes</topic><topic>Diseases</topic><topic>DNA Barcoding, Taxonomic</topic><topic>DNA repair</topic><topic>Drinking water</topic><topic>Environmental aspects</topic><topic>Environmental health</topic><topic>Experiments</topic><topic>Exposure</topic><topic>Female</topic><topic>Gastrointestinal Tract - drug effects</topic><topic>Gastrointestinal Tract - microbiology</topic><topic>Gene expression</topic><topic>Gene sequencing</topic><topic>Genomes</topic><topic>Health</topic><topic>Health aspects</topic><topic>Human</topic><topic>Human populations</topic><topic>Integrated approach</topic><topic>Laboratory animals</topic><topic>Mass Spectrometry</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Metabolome</topic><topic>Metagenome - drug effects</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microbiota</topic><topic>Microbiota (Symbiotic organisms)</topic><topic>Microbiota - drug effects</topic><topic>Molecular Sequence Data</topic><topic>PCB</topic><topic>Polychlorinated biphenyls</topic><topic>Polycyclic aromatic hydrocarbons</topic><topic>Profiling</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>RNA, Ribosomal, 16S - metabolism</topic><topic>Scientific imaging</topic><topic>Sequence Analysis, DNA</topic><topic>Signal transduction</topic><topic>Software</topic><topic>Specific Pathogen-Free Organisms</topic><topic>Studies</topic><topic>Taxonomy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Kun</creatorcontrib><creatorcontrib>Abo, Ryan Phillip</creatorcontrib><creatorcontrib>Schlieper, Katherine Ann</creatorcontrib><creatorcontrib>Graffam, Michelle E</creatorcontrib><creatorcontrib>Levine, Stuart</creatorcontrib><creatorcontrib>Wishnok, John S</creatorcontrib><creatorcontrib>Swenberg, James A</creatorcontrib><creatorcontrib>Tannenbaum, Steven R</creatorcontrib><creatorcontrib>Fox, James G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In 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G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Arsenic exposure perturbs the gut microbiome and its metabolic profile in mice: an integrated metagenomics and metabolomics analysis</atitle><jtitle>Environmental health perspectives</jtitle><addtitle>Environ Health Perspect</addtitle><date>2014-03-01</date><risdate>2014</risdate><volume>122</volume><issue>3</issue><spage>284</spage><epage>291</epage><pages>284-291</pages><issn>0091-6765</issn><eissn>1552-9924</eissn><abstract>The human intestine is host to an enormously complex, diverse, and vast microbial community-the gut microbiota. The gut microbiome plays a profound role in metabolic processing, energy production, immune and cognitive development, epithelial homeostasis, and so forth. However, the composition and diversity of the gut microbiome can be readily affected by external factors, which raises the possibility that exposure to toxic environmental chemicals leads to gut microbiome alteration, or dysbiosis. Arsenic exposure affects large human populations worldwide and has been linked to a number of diseases, including cancer, diabetes, and cardiovascular disorders.
We investigated the impact of arsenic exposure on the gut microbiome composition and its metabolic profiles.
We used an integrated approach combining 16S rRNA gene sequencing and mass spectrometry-based metabolomics profiling to examine the functional impact of arsenic exposure on the gut microbiome.
16S rRNA gene sequencing revealed that arsenic significantly perturbed the gut microbiome composition in C57BL/6 mice after exposure to 10 ppm arsenic for 4 weeks in drinking water. Moreover, metabolomics profiling revealed a concurrent effect, with a number of gut microflora-related metabolites being perturbed in multiple biological matrices.
Arsenic exposure not only alters the gut microbiome community at the abundance level but also substantially disturbs its metabolic profiles at the function level. These findings may provide novel insights regarding perturbations of the gut microbiome and its functions as a potential new mechanism by which arsenic exposure leads to or exacerbates human diseases.
Lu K, Abo RP, Schlieper KA, Graffam ME, Levine S, Wishnok JS, Swenberg JA, Tannenbaum SR, Fox JG. 2014. Arsenic exposure perturbs the gut microbiome and its metabolic profile in mice: an integrated metagenomics and metabolomics analysis. Environ Health Perspect 122:284-291; http://dx.doi.org/10.1289/ehp.1307429.</abstract><cop>United States</cop><pub>National Institute of Environmental Health Sciences</pub><pmid>24413286</pmid><doi>10.1289/ehp.1307429</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0091-6765 |
| ispartof | Environmental health perspectives, 2014-03, Vol.122 (3), p.284-291 |
| issn | 0091-6765 1552-9924 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3948040 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central Open Access; Jstor Complete Legacy; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Arsenic Arsenic - toxicity Bacteria Chromatography Chromatography, Liquid Diabetes Diseases DNA Barcoding, Taxonomic DNA repair Drinking water Environmental aspects Environmental health Experiments Exposure Female Gastrointestinal Tract - drug effects Gastrointestinal Tract - microbiology Gene expression Gene sequencing Genomes Health Health aspects Human Human populations Integrated approach Laboratory animals Mass Spectrometry Metabolism Metabolites Metabolome Metagenome - drug effects Mice Mice, Inbred C57BL Microbiota Microbiota (Symbiotic organisms) Microbiota - drug effects Molecular Sequence Data PCB Polychlorinated biphenyls Polycyclic aromatic hydrocarbons Profiling RNA, Ribosomal, 16S - genetics RNA, Ribosomal, 16S - metabolism Scientific imaging Sequence Analysis, DNA Signal transduction Software Specific Pathogen-Free Organisms Studies Taxonomy |
| title | Arsenic exposure perturbs the gut microbiome and its metabolic profile in mice: an integrated metagenomics and metabolomics analysis |
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