Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis in Xenopus tropicalis

We have assessed the efficacy of the recently developed CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR‐associated) system for genome modification in the amphibian Xenopus tropicalis. As a model experiment, targeted mutations of the tyrosinase gene were verified, showing...

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Veröffentlicht in:	Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2013-12, Vol.51 (12), p.835-843
Hauptverfasser:	Nakayama, Takuya, Fish, Margaret B., Fisher, Marilyn, Oomen-Hajagos, Jamina, Thomsen, Gerald H., Grainger, Robert M.
Format:	Artikel
Sprache:	eng
Schlagworte:	albinism Animals brain Brain - metabolism Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-Cas Systems Embryo, Nonmammalian - metabolism eye Eye - metabolism Eye Proteins - genetics Eye Proteins - metabolism Genetic Loci Genome Germ Cells - metabolism Homeobox Protein SIX3 Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Mutagenesis, Site-Directed Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Phenotype sgRNA six3 tyrosinase Xenopus Xenopus - embryology Xenopus - genetics Xenopus Proteins - genetics Xenopus Proteins - metabolism Xenopus tropicalis
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