Application of recombinant human leukemia inhibitory factor (LIF) produced in rice (Oryza sativa L.) for maintenance of mouse embryonic stem cells
Embryonic and induced pluripotent stem cells have the ability to differentiate into any somatic cell type, and thus have potential to treat a number of diseases that are currently incurable. Application of these cells for clinical or industrial uses would require an increase in production to yield a...
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| Veröffentlicht in: | Journal of biotechnology 2014-02, Vol.172, p.67-72 |
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| container_title | Journal of biotechnology |
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| creator | Youngblood, Bradford A Alfano, Randall Pettit, Steve C Zhang, Deshui Dallmann, H Garry Huang, Ning Macdonald, Clinton C |
| description | Embryonic and induced pluripotent stem cells have the ability to differentiate into any somatic cell type, and thus have potential to treat a number of diseases that are currently incurable. Application of these cells for clinical or industrial uses would require an increase in production to yield adequate numbers of viable cells. However, the relatively high costs of cytokines and growth factors required for maintenance of stem cells in the undifferentiated state have the potential to limit translational research. Leukemia inhibitory factor (LIF), a member of the IL-6 cytokine family, is a key regulator in the maintenance of naïve states for both human and mouse stem cells. In this study, we describe a new recombinant human LIF (rhLIF) using a plant-based (rice) expression system. We found that rice-derived rhLIF possessed the same specific activity as commercial Escherichia coli-derived LIF and was capable of supporting mouse embryonic stem cell proliferation in the undifferentiated state as evidenced from pluripotency marker level analysis. Retention of the pluripotent state was found to be indistinguishable between rice-derived rhLIF and other recombinant LIF proteins currently on the market. |
| doi_str_mv | 10.1016/j.jbiotec.2013.12.012 |
| format | Article |
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Application of these cells for clinical or industrial uses would require an increase in production to yield adequate numbers of viable cells. However, the relatively high costs of cytokines and growth factors required for maintenance of stem cells in the undifferentiated state have the potential to limit translational research. Leukemia inhibitory factor (LIF), a member of the IL-6 cytokine family, is a key regulator in the maintenance of naïve states for both human and mouse stem cells. In this study, we describe a new recombinant human LIF (rhLIF) using a plant-based (rice) expression system. We found that rice-derived rhLIF possessed the same specific activity as commercial Escherichia coli-derived LIF and was capable of supporting mouse embryonic stem cell proliferation in the undifferentiated state as evidenced from pluripotency marker level analysis. Retention of the pluripotent state was found to be indistinguishable between rice-derived rhLIF and other recombinant LIF proteins currently on the market.</description><identifier>ISSN: 0168-1656</identifier><identifier>EISSN: 1873-4863</identifier><identifier>DOI: 10.1016/j.jbiotec.2013.12.012</identifier><identifier>PMID: 24380819</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Animals ; Biotechnology ; Cell Proliferation ; cell viability ; Cells, Cultured ; Cytokines ; Embryo, Mammalian ; embryonic stem cells ; Embryonic Stem Cells - immunology ; Escherichia ; growth factors ; Human ; Humans ; induced pluripotent stem cells ; Induced Pluripotent Stem Cells - immunology ; leukemia inhibitory factor ; Leukemia Inhibitory Factor - metabolism ; Leukemias ; Maintenance ; Markers ; markets ; Mice ; Mice, Inbred C57BL ; Oryza - genetics ; Oryza - metabolism ; Oryza sativa ; Plants, Genetically Modified ; proteins ; Recombinant ; Recombinant Proteins - metabolism ; rice ; somatic cells ; Stem cells ; Translational Medical Research</subject><ispartof>Journal of biotechnology, 2014-02, Vol.172, p.67-72</ispartof><rights>Copyright © 2013 The Authors. Published by Elsevier B.V. All rights reserved.</rights><rights>2014 The Authors. Published by Elsevier B.V. All rights reserved. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4252-3876d3a84b8ee3aaa544b7dfaf51cba9cc984ea79f9c715e653d42663dca8dc83</citedby><cites>FETCH-LOGICAL-c4252-3876d3a84b8ee3aaa544b7dfaf51cba9cc984ea79f9c715e653d42663dca8dc83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24380819$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Youngblood, Bradford A</creatorcontrib><creatorcontrib>Alfano, Randall</creatorcontrib><creatorcontrib>Pettit, Steve C</creatorcontrib><creatorcontrib>Zhang, Deshui</creatorcontrib><creatorcontrib>Dallmann, H Garry</creatorcontrib><creatorcontrib>Huang, Ning</creatorcontrib><creatorcontrib>Macdonald, Clinton C</creatorcontrib><title>Application of recombinant human leukemia inhibitory factor (LIF) produced in rice (Oryza sativa L.) for maintenance of mouse embryonic stem cells</title><title>Journal of biotechnology</title><addtitle>J Biotechnol</addtitle><description>Embryonic and induced pluripotent stem cells have the ability to differentiate into any somatic cell type, and thus have potential to treat a number of diseases that are currently incurable. Application of these cells for clinical or industrial uses would require an increase in production to yield adequate numbers of viable cells. However, the relatively high costs of cytokines and growth factors required for maintenance of stem cells in the undifferentiated state have the potential to limit translational research. Leukemia inhibitory factor (LIF), a member of the IL-6 cytokine family, is a key regulator in the maintenance of naïve states for both human and mouse stem cells. In this study, we describe a new recombinant human LIF (rhLIF) using a plant-based (rice) expression system. We found that rice-derived rhLIF possessed the same specific activity as commercial Escherichia coli-derived LIF and was capable of supporting mouse embryonic stem cell proliferation in the undifferentiated state as evidenced from pluripotency marker level analysis. Retention of the pluripotent state was found to be indistinguishable between rice-derived rhLIF and other recombinant LIF proteins currently on the market.</description><subject>Animals</subject><subject>Biotechnology</subject><subject>Cell Proliferation</subject><subject>cell viability</subject><subject>Cells, Cultured</subject><subject>Cytokines</subject><subject>Embryo, Mammalian</subject><subject>embryonic stem cells</subject><subject>Embryonic Stem Cells - immunology</subject><subject>Escherichia</subject><subject>growth factors</subject><subject>Human</subject><subject>Humans</subject><subject>induced pluripotent stem cells</subject><subject>Induced Pluripotent Stem Cells - immunology</subject><subject>leukemia inhibitory factor</subject><subject>Leukemia Inhibitory Factor - metabolism</subject><subject>Leukemias</subject><subject>Maintenance</subject><subject>Markers</subject><subject>markets</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Oryza - genetics</subject><subject>Oryza - metabolism</subject><subject>Oryza sativa</subject><subject>Plants, Genetically Modified</subject><subject>proteins</subject><subject>Recombinant</subject><subject>Recombinant Proteins - metabolism</subject><subject>rice</subject><subject>somatic cells</subject><subject>Stem cells</subject><subject>Translational Medical Research</subject><issn>0168-1656</issn><issn>1873-4863</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFksFu1DAQhi0EokvhEUA-bg8JcWwn9gWpqihUWqkXOFsTZ8J6SezFTiptH4MnxqsuFZz2NAd_83v-mZ-Q96wqWcWaj7ty17kwoy3rivGS1WXF6hdkxVTLC6Ea_pKsMqcK1sjmgrxJaVdVldCSvSYXteCqUkyvyO_r_X50FmYXPA0DjWjD1DkPfqbbZQJPR1x-4uSAOr91nZtDPNABbK50vbm7vaL7GPrFYp8BGp1Fur6Ph0egKYs-AN2UV3TI8ATOz5iFM5E_msKSkOLUxUPwztI040QtjmN6S14NMCZ8d6qX5Pvt5283X4vN_Ze7m-tNYUUt64Krtuk5KNEpRA4AUoiu7QcYJLMdaGu1EgitHrRtmcRG8l7UTcN7C6q3il-ST0-6-6WbsLfo5wij2Uc3QTyYAM78_-Ld1vwID4Zr0Qqts8D6JBDDrwXTbCaXjhbAY3Zn6rzwfAElxFk0Y7LVrMmnPI-2DZeKyfMDsDwlY22tZUblE2pjSCni8OyTVeYYKLMzp0CZY6AMq00OVO778O-Snrv-Joj_AWPyy6w</recordid><startdate>20140220</startdate><enddate>20140220</enddate><creator>Youngblood, Bradford A</creator><creator>Alfano, Randall</creator><creator>Pettit, Steve C</creator><creator>Zhang, Deshui</creator><creator>Dallmann, H Garry</creator><creator>Huang, Ning</creator><creator>Macdonald, Clinton C</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7U5</scope><scope>L7M</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20140220</creationdate><title>Application of recombinant human leukemia inhibitory factor (LIF) produced in rice (Oryza sativa L.) for maintenance of mouse embryonic stem cells</title><author>Youngblood, Bradford A ; Alfano, Randall ; Pettit, Steve C ; Zhang, Deshui ; Dallmann, H Garry ; Huang, Ning ; Macdonald, Clinton C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4252-3876d3a84b8ee3aaa544b7dfaf51cba9cc984ea79f9c715e653d42663dca8dc83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Biotechnology</topic><topic>Cell Proliferation</topic><topic>cell viability</topic><topic>Cells, Cultured</topic><topic>Cytokines</topic><topic>Embryo, Mammalian</topic><topic>embryonic stem cells</topic><topic>Embryonic Stem Cells - immunology</topic><topic>Escherichia</topic><topic>growth factors</topic><topic>Human</topic><topic>Humans</topic><topic>induced pluripotent stem cells</topic><topic>Induced Pluripotent Stem Cells - immunology</topic><topic>leukemia inhibitory factor</topic><topic>Leukemia Inhibitory Factor - metabolism</topic><topic>Leukemias</topic><topic>Maintenance</topic><topic>Markers</topic><topic>markets</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Oryza - genetics</topic><topic>Oryza - metabolism</topic><topic>Oryza sativa</topic><topic>Plants, Genetically Modified</topic><topic>proteins</topic><topic>Recombinant</topic><topic>Recombinant Proteins - metabolism</topic><topic>rice</topic><topic>somatic cells</topic><topic>Stem cells</topic><topic>Translational Medical Research</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Youngblood, Bradford A</creatorcontrib><creatorcontrib>Alfano, Randall</creatorcontrib><creatorcontrib>Pettit, Steve C</creatorcontrib><creatorcontrib>Zhang, Deshui</creatorcontrib><creatorcontrib>Dallmann, H Garry</creatorcontrib><creatorcontrib>Huang, Ning</creatorcontrib><creatorcontrib>Macdonald, Clinton C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Youngblood, Bradford A</au><au>Alfano, Randall</au><au>Pettit, Steve C</au><au>Zhang, Deshui</au><au>Dallmann, H Garry</au><au>Huang, Ning</au><au>Macdonald, Clinton C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of recombinant human leukemia inhibitory factor (LIF) produced in rice (Oryza sativa L.) for maintenance of mouse embryonic stem cells</atitle><jtitle>Journal of biotechnology</jtitle><addtitle>J Biotechnol</addtitle><date>2014-02-20</date><risdate>2014</risdate><volume>172</volume><spage>67</spage><epage>72</epage><pages>67-72</pages><issn>0168-1656</issn><eissn>1873-4863</eissn><abstract>Embryonic and induced pluripotent stem cells have the ability to differentiate into any somatic cell type, and thus have potential to treat a number of diseases that are currently incurable. 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| ispartof | Journal of biotechnology, 2014-02, Vol.172, p.67-72 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biotechnology Cell Proliferation cell viability Cells, Cultured Cytokines Embryo, Mammalian embryonic stem cells Embryonic Stem Cells - immunology Escherichia growth factors Human Humans induced pluripotent stem cells Induced Pluripotent Stem Cells - immunology leukemia inhibitory factor Leukemia Inhibitory Factor - metabolism Leukemias Maintenance Markers markets Mice Mice, Inbred C57BL Oryza - genetics Oryza - metabolism Oryza sativa Plants, Genetically Modified proteins Recombinant Recombinant Proteins - metabolism rice somatic cells Stem cells Translational Medical Research |
| title | Application of recombinant human leukemia inhibitory factor (LIF) produced in rice (Oryza sativa L.) for maintenance of mouse embryonic stem cells |
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