A potassium transporter of the yeast Schwanniomyces occidentalis homologous to the Kup system of Escherichia coli has a high concentrative capacity
The yeast Schwanniomyces occidentalis has a high‐affinity K+ uptake system with a high concentrative capacity, which is able to deplete the external K+ to < 0.03 microM. We have cloned the gene HAK1 of S.occidentalis which complements defective K+ uptake by trk1 and trk1 trk2 mutants of Saccharom...
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| Veröffentlicht in: | The EMBO journal 1995-07, Vol.14 (13), p.3021-3027 |
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| creator | Bañuelos, M.A. Klein, R.D. Alexander‐Bowman, S.J. Rodríguez‐Navarro, A. |
| description | The yeast Schwanniomyces occidentalis has a high‐affinity K+ uptake system with a high concentrative capacity, which is able to deplete the external K+ to < 0.03 microM. We have cloned the gene HAK1 of S.occidentalis which complements defective K+ uptake by trk1 and trk1 trk2 mutants of Saccharomyces cerevisiae. When HAK1 was expressed in a trk1 trk2 S.cerevisiae mutant, transport affinities for K+ and other alkali cations resembled those of S.occidentalis. The predicted amino acid sequence of the HAK1 protein shows significant homology with the hydrophobic region of the Kup transporter of Escherichia coli. In S.occidentalis HAK1 expresses in K(+)‐limiting conditions. Our data indicate that in K(+)‐starved cells the system encoded by HAK1 is the major K+ transporter of S.occidentalis. |
| doi_str_mv | 10.1002/j.1460-2075.1995.tb07304.x |
| format | Article |
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We have cloned the gene HAK1 of S.occidentalis which complements defective K+ uptake by trk1 and trk1 trk2 mutants of Saccharomyces cerevisiae. When HAK1 was expressed in a trk1 trk2 S.cerevisiae mutant, transport affinities for K+ and other alkali cations resembled those of S.occidentalis. The predicted amino acid sequence of the HAK1 protein shows significant homology with the hydrophobic region of the Kup transporter of Escherichia coli. In S.occidentalis HAK1 expresses in K(+)‐limiting conditions. Our data indicate that in K(+)‐starved cells the system encoded by HAK1 is the major K+ transporter of S.occidentalis.</description><identifier>ISSN: 0261-4189</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1002/j.1460-2075.1995.tb07304.x</identifier><identifier>PMID: 7621817</identifier><language>eng</language><publisher>England</publisher><subject>Amino Acid Sequence ; Ascomycota - drug effects ; Ascomycota - genetics ; Ascomycota - metabolism ; Escherichia coli - metabolism ; Gene Expression Regulation, Fungal - drug effects ; Genes, Fungal - drug effects ; Glucose - pharmacology ; Ion Transport - drug effects ; Kinetics ; Molecular Sequence Data ; Mutation ; Neomycin - pharmacology ; Potassium - pharmacokinetics ; Rubidium - pharmacokinetics ; Saccharomyces cerevisiae ; Schwanniomyces occidentalis</subject><ispartof>The EMBO journal, 1995-07, Vol.14 (13), p.3021-3027</ispartof><rights>1995 European Molecular Biology Organization</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5754-e2f55f169261138a4e058fbeebe9c75ab01afba7138f0a6cc9c7b81e8b207e8f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC394362/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC394362/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7621817$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bañuelos, M.A.</creatorcontrib><creatorcontrib>Klein, R.D.</creatorcontrib><creatorcontrib>Alexander‐Bowman, S.J.</creatorcontrib><creatorcontrib>Rodríguez‐Navarro, A.</creatorcontrib><title>A potassium transporter of the yeast Schwanniomyces occidentalis homologous to the Kup system of Escherichia coli has a high concentrative capacity</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><description>The yeast Schwanniomyces occidentalis has a high‐affinity K+ uptake system with a high concentrative capacity, which is able to deplete the external K+ to < 0.03 microM. We have cloned the gene HAK1 of S.occidentalis which complements defective K+ uptake by trk1 and trk1 trk2 mutants of Saccharomyces cerevisiae. When HAK1 was expressed in a trk1 trk2 S.cerevisiae mutant, transport affinities for K+ and other alkali cations resembled those of S.occidentalis. The predicted amino acid sequence of the HAK1 protein shows significant homology with the hydrophobic region of the Kup transporter of Escherichia coli. In S.occidentalis HAK1 expresses in K(+)‐limiting conditions. Our data indicate that in K(+)‐starved cells the system encoded by HAK1 is the major K+ transporter of S.occidentalis.</description><subject>Amino Acid Sequence</subject><subject>Ascomycota - drug effects</subject><subject>Ascomycota - genetics</subject><subject>Ascomycota - metabolism</subject><subject>Escherichia coli - metabolism</subject><subject>Gene Expression Regulation, Fungal - drug effects</subject><subject>Genes, Fungal - drug effects</subject><subject>Glucose - pharmacology</subject><subject>Ion Transport - drug effects</subject><subject>Kinetics</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Neomycin - pharmacology</subject><subject>Potassium - pharmacokinetics</subject><subject>Rubidium - pharmacokinetics</subject><subject>Saccharomyces cerevisiae</subject><subject>Schwanniomyces occidentalis</subject><issn>0261-4189</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkc1u1DAUhS0EKkPhEZAsFuyS2vlzgsRiWg1toYgFsLZu3JuJR0kcbKdtnoMXrtMZjWDZlX-Oz_G99yPkA2cxZyw528U8K1iUMJHHvKry2NdMpCyLH16Q1VF6SVYsKXiU8bJ6Td44t2OM5aXgJ-REFAkvuViRv2s6Gg_O6amn3sLgRmM9Wmoa6lukM4Lz9Kdq72EYtOlnhY4apfQtDh467WhretOZrZkc9ebJ820aqZudx35J2TjVotWq1UCV6TRtwVGgrd624TyokGPB6zukCkZQ2s9vyasGOofvDusp-f1l8-viKrr5cXl9sb6JVC7yLMKkyfOGF1VokqclZBi6a2rEGislcqgZh6YGEbSGQaFUuK1LjmUdpoNlk56Sz_vccap7vN1X0snR6h7sLA1o-b8y6FZuzZ1MqywtkuD_ePBb82dC52WvncKugwHDOCQvypQnQoSHn_YPlTXOWWyOf3AmF6JyJxdscsEmF6LyQFQ-BPP7f6s8Wg8Ig77e6_e6w_kZyXLz_fzr0z59BJ_ut3o</recordid><startdate>19950703</startdate><enddate>19950703</enddate><creator>Bañuelos, M.A.</creator><creator>Klein, R.D.</creator><creator>Alexander‐Bowman, S.J.</creator><creator>Rodríguez‐Navarro, A.</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>19950703</creationdate><title>A potassium transporter of the yeast Schwanniomyces occidentalis homologous to the Kup system of Escherichia coli has a high concentrative capacity</title><author>Bañuelos, M.A. ; Klein, R.D. ; Alexander‐Bowman, S.J. ; Rodríguez‐Navarro, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5754-e2f55f169261138a4e058fbeebe9c75ab01afba7138f0a6cc9c7b81e8b207e8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>Ascomycota - drug effects</topic><topic>Ascomycota - genetics</topic><topic>Ascomycota - metabolism</topic><topic>Escherichia coli - metabolism</topic><topic>Gene Expression Regulation, Fungal - drug effects</topic><topic>Genes, Fungal - drug effects</topic><topic>Glucose - pharmacology</topic><topic>Ion Transport - drug effects</topic><topic>Kinetics</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Neomycin - pharmacology</topic><topic>Potassium - pharmacokinetics</topic><topic>Rubidium - pharmacokinetics</topic><topic>Saccharomyces cerevisiae</topic><topic>Schwanniomyces occidentalis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bañuelos, M.A.</creatorcontrib><creatorcontrib>Klein, R.D.</creatorcontrib><creatorcontrib>Alexander‐Bowman, S.J.</creatorcontrib><creatorcontrib>Rodríguez‐Navarro, A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bañuelos, M.A.</au><au>Klein, R.D.</au><au>Alexander‐Bowman, S.J.</au><au>Rodríguez‐Navarro, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A potassium transporter of the yeast Schwanniomyces occidentalis homologous to the Kup system of Escherichia coli has a high concentrative capacity</atitle><jtitle>The EMBO journal</jtitle><addtitle>EMBO J</addtitle><date>1995-07-03</date><risdate>1995</risdate><volume>14</volume><issue>13</issue><spage>3021</spage><epage>3027</epage><pages>3021-3027</pages><issn>0261-4189</issn><eissn>1460-2075</eissn><abstract>The yeast Schwanniomyces occidentalis has a high‐affinity K+ uptake system with a high concentrative capacity, which is able to deplete the external K+ to < 0.03 microM. We have cloned the gene HAK1 of S.occidentalis which complements defective K+ uptake by trk1 and trk1 trk2 mutants of Saccharomyces cerevisiae. When HAK1 was expressed in a trk1 trk2 S.cerevisiae mutant, transport affinities for K+ and other alkali cations resembled those of S.occidentalis. The predicted amino acid sequence of the HAK1 protein shows significant homology with the hydrophobic region of the Kup transporter of Escherichia coli. In S.occidentalis HAK1 expresses in K(+)‐limiting conditions. Our data indicate that in K(+)‐starved cells the system encoded by HAK1 is the major K+ transporter of S.occidentalis.</abstract><cop>England</cop><pmid>7621817</pmid><doi>10.1002/j.1460-2075.1995.tb07304.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The EMBO journal, 1995-07, Vol.14 (13), p.3021-3027 |
| issn | 0261-4189 1460-2075 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Ascomycota - drug effects Ascomycota - genetics Ascomycota - metabolism Escherichia coli - metabolism Gene Expression Regulation, Fungal - drug effects Genes, Fungal - drug effects Glucose - pharmacology Ion Transport - drug effects Kinetics Molecular Sequence Data Mutation Neomycin - pharmacology Potassium - pharmacokinetics Rubidium - pharmacokinetics Saccharomyces cerevisiae Schwanniomyces occidentalis |
| title | A potassium transporter of the yeast Schwanniomyces occidentalis homologous to the Kup system of Escherichia coli has a high concentrative capacity |
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