Exogenous carbon monoxide suppresses adaptive response induced in zebrafish embryos in vivo by microbeam protons
Dechorionated embryos of the zebrafish, Danio rerio, irradiated at 5 h post-fertilization (hpf) with 30 protons delivered to 10 separate positions each with an energy of 3.4 MeV from the microbeam irradiation facility (Single-Particle Irradiation System to Cell, acronym as SPICE) at the National Ins...
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| Veröffentlicht in: | Journal of radiation research 2014-03, Vol.55 (suppl_1), p.i115-i115 |
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| container_title | Journal of radiation research |
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| creator | Choi, Viann Wing Yan Ng, Candy Yuen Ping Kobayashi, Alisa Konishi, Teruaki Oikawa, Masakazu Cheng, Shuk Han Yu, Peter Kwan Ngok |
| description | Dechorionated embryos of the zebrafish, Danio rerio, irradiated at 5 h post-fertilization (hpf) with 30 protons delivered to 10 separate positions each with an energy of 3.4 MeV from the microbeam irradiation facility (Single-Particle Irradiation System to Cell, acronym as SPICE) at the National Institute of Radiological Sciences (NIRS), developed radioadaptive response (RAR) against a subsequent challenging exposure of 2 Gy of X-ray irradiation at 10 hpf, corroborated by reduced apoptotic signals at 25 hpf revealed through terminal dUTP transferase-mediated nick end-labeling assay.
The effects of the CO liberator tricarbonylchloro(glycinato)ruthenium (II) (CORM-3) on the induction of RAR were examined by transferring the irradiated embryos to freshly prepared medium with the chemical at different time points after the application of the priming dose. Our results showed that transfer of irradiated embryos into media with CORM-3 at 0, 1, 2 and 3 h after application of priming exposure significantly suppressed RAR, while transfer at 5 h did not suppress RAR. This was attributed to the protection of bystander cells from the released CO, which caused less de novo synthesis of factors and thus less efficient induction of RAR. Once the factors were synthesized, RAR was induced, which would not be further affected by the application of CORM-3 introduced at 5 h after the application of the priming dose.
Clinical Trial Registration number if required: None. |
| doi_str_mv | 10.1093/jrr/rrt165 |
| format | Article |
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The effects of the CO liberator tricarbonylchloro(glycinato)ruthenium (II) (CORM-3) on the induction of RAR were examined by transferring the irradiated embryos to freshly prepared medium with the chemical at different time points after the application of the priming dose. Our results showed that transfer of irradiated embryos into media with CORM-3 at 0, 1, 2 and 3 h after application of priming exposure significantly suppressed RAR, while transfer at 5 h did not suppress RAR. This was attributed to the protection of bystander cells from the released CO, which caused less de novo synthesis of factors and thus less efficient induction of RAR. Once the factors were synthesized, RAR was induced, which would not be further affected by the application of CORM-3 introduced at 5 h after the application of the priming dose.
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The effects of the CO liberator tricarbonylchloro(glycinato)ruthenium (II) (CORM-3) on the induction of RAR were examined by transferring the irradiated embryos to freshly prepared medium with the chemical at different time points after the application of the priming dose. Our results showed that transfer of irradiated embryos into media with CORM-3 at 0, 1, 2 and 3 h after application of priming exposure significantly suppressed RAR, while transfer at 5 h did not suppress RAR. This was attributed to the protection of bystander cells from the released CO, which caused less de novo synthesis of factors and thus less efficient induction of RAR. Once the factors were synthesized, RAR was induced, which would not be further affected by the application of CORM-3 introduced at 5 h after the application of the priming dose.
Clinical Trial Registration number if required: None.</description><subject>Apoptosis</subject><subject>Carbon monoxide</subject><subject>Danio rerio</subject><subject>Embryonic development</subject><subject>Embryos</subject><subject>Exposure</subject><subject>Food processing plants</subject><subject>Irradiation</subject><subject>Microbeams</subject><subject>Platinum group compounds</subject><subject>Priming</subject><subject>Protons</subject><subject>Terminals</subject><subject>Zebrafish</subject><issn>0449-3060</issn><issn>1349-9157</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>TOX</sourceid><recordid>eNqNkl1rHCEUhiU00G3am_4CoRRKYBMddWa9KYSQtIFAb9Jr8ePMxjCjVmeWbH99XCYEAqUEkaPH5xz15UXoMyVnlEh2_pDzec4TbcURWlHG5VpS0b1DK8LrmpGWvEcfSnkgpOmIICuUrh7jFkKcC7Y6mxjwGEN89A5wmVPKUAoUrJ1Ok98BrvsUQwHsg5stuBrxXzBZ977cYxhN3sdySO78LmKzx6O3ORrQI045TrX0Izru9VDg03M8Qb-vr-4uf65vf_24uby4XVvWcbFuGiudkMzwznaScMGpJhtjBIATtJGc0E40dsOJZkLo3mmuKVjtrBVU0padoO9L3zSbEZyFMGU9qJT9qPNeRe3V65Pg79U27hSTnAohaoNvzw1y_DNDmdToi4Vh0AGqXIp2kjX1IQ19A9oK2ckN4xX9sqBbPYDyoY_1cnvA1YVgLT1MVqmzf1B1OKiCxgC9r_lXBadLQVW7lAz9y0cpUQdnqOoMtTijwl8XOM7pf9wTwdq7mw</recordid><startdate>20140301</startdate><enddate>20140301</enddate><creator>Choi, Viann Wing Yan</creator><creator>Ng, Candy Yuen Ping</creator><creator>Kobayashi, Alisa</creator><creator>Konishi, Teruaki</creator><creator>Oikawa, Masakazu</creator><creator>Cheng, Shuk Han</creator><creator>Yu, Peter Kwan Ngok</creator><general>Oxford University Press</general><scope>TOX</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7SU</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>L7M</scope><scope>5PM</scope></search><sort><creationdate>20140301</creationdate><title>Exogenous carbon monoxide suppresses adaptive response induced in zebrafish embryos in vivo by microbeam protons</title><author>Choi, Viann Wing Yan ; Ng, Candy Yuen Ping ; Kobayashi, Alisa ; Konishi, Teruaki ; Oikawa, Masakazu ; Cheng, Shuk Han ; Yu, Peter Kwan Ngok</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3745-22c9d593b47c7904541a08bb5eed5129401752c840a355afda4a1ecadcc519163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Apoptosis</topic><topic>Carbon monoxide</topic><topic>Danio rerio</topic><topic>Embryonic development</topic><topic>Embryos</topic><topic>Exposure</topic><topic>Food processing plants</topic><topic>Irradiation</topic><topic>Microbeams</topic><topic>Platinum group compounds</topic><topic>Priming</topic><topic>Protons</topic><topic>Terminals</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choi, Viann Wing Yan</creatorcontrib><creatorcontrib>Ng, Candy Yuen Ping</creatorcontrib><creatorcontrib>Kobayashi, Alisa</creatorcontrib><creatorcontrib>Konishi, Teruaki</creatorcontrib><creatorcontrib>Oikawa, Masakazu</creatorcontrib><creatorcontrib>Cheng, Shuk Han</creatorcontrib><creatorcontrib>Yu, Peter Kwan Ngok</creatorcontrib><collection>Oxford Journals Open Access Collection</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Environmental Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of radiation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choi, Viann Wing Yan</au><au>Ng, Candy Yuen Ping</au><au>Kobayashi, Alisa</au><au>Konishi, Teruaki</au><au>Oikawa, Masakazu</au><au>Cheng, Shuk Han</au><au>Yu, Peter Kwan Ngok</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exogenous carbon monoxide suppresses adaptive response induced in zebrafish embryos in vivo by microbeam protons</atitle><jtitle>Journal of radiation research</jtitle><date>2014-03-01</date><risdate>2014</risdate><volume>55</volume><issue>suppl_1</issue><spage>i115</spage><epage>i115</epage><pages>i115-i115</pages><issn>0449-3060</issn><eissn>1349-9157</eissn><abstract>Dechorionated embryos of the zebrafish, Danio rerio, irradiated at 5 h post-fertilization (hpf) with 30 protons delivered to 10 separate positions each with an energy of 3.4 MeV from the microbeam irradiation facility (Single-Particle Irradiation System to Cell, acronym as SPICE) at the National Institute of Radiological Sciences (NIRS), developed radioadaptive response (RAR) against a subsequent challenging exposure of 2 Gy of X-ray irradiation at 10 hpf, corroborated by reduced apoptotic signals at 25 hpf revealed through terminal dUTP transferase-mediated nick end-labeling assay.
The effects of the CO liberator tricarbonylchloro(glycinato)ruthenium (II) (CORM-3) on the induction of RAR were examined by transferring the irradiated embryos to freshly prepared medium with the chemical at different time points after the application of the priming dose. Our results showed that transfer of irradiated embryos into media with CORM-3 at 0, 1, 2 and 3 h after application of priming exposure significantly suppressed RAR, while transfer at 5 h did not suppress RAR. This was attributed to the protection of bystander cells from the released CO, which caused less de novo synthesis of factors and thus less efficient induction of RAR. Once the factors were synthesized, RAR was induced, which would not be further affected by the application of CORM-3 introduced at 5 h after the application of the priming dose.
Clinical Trial Registration number if required: None.</abstract><pub>Oxford University Press</pub><doi>10.1093/jrr/rrt165</doi><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Oxford Journals Open Access Collection; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Apoptosis Carbon monoxide Danio rerio Embryonic development Embryos Exposure Food processing plants Irradiation Microbeams Platinum group compounds Priming Protons Terminals Zebrafish |
| title | Exogenous carbon monoxide suppresses adaptive response induced in zebrafish embryos in vivo by microbeam protons |
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