Insights into naturally minimised Streptomyces albus J1074 genome
The Streptomyces albus J1074 strain is one of the most widely used chassis for the heterologous production of bioactive natural products. The fast growth and an efficient genetic system make this strain an attractive model for expressing cryptic biosynthetic pathways to aid drug discovery. To improv...
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| Veröffentlicht in: | BMC genomics 2014-02, Vol.15 (1), p.97-97, Article 97 |
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| creator | Zaburannyi, Nestor Rabyk, Mariia Ostash, Bohdan Fedorenko, Victor Luzhetskyy, Andriy |
| description | The Streptomyces albus J1074 strain is one of the most widely used chassis for the heterologous production of bioactive natural products. The fast growth and an efficient genetic system make this strain an attractive model for expressing cryptic biosynthetic pathways to aid drug discovery.
To improve its capabilities for the heterologous expression of biosynthetic gene clusters, the complete genomic sequence of S. albus J1074 was obtained. With a size of 6,841,649 bp, coding for 5,832 genes, its genome is the smallest within the genus streptomycetes. Genome analysis revealed a strong tendency to reduce the number of genetic duplicates. The whole transcriptomes were sequenced at different time points to identify the early metabolic switch from the exponential to the stationary phase in S. albus J1074.
S. albus J1074 carries the smallest genome among the completely sequenced species of the genus Streptomyces. The detailed genome and transcriptome analysis discloses its capability to serve as a premium host for the heterologous production of natural products. Moreover, the genome revealed 22 additional putative secondary metabolite gene clusters that reinforce the strain's potential for natural product synthesis. |
| doi_str_mv | 10.1186/1471-2164-15-97 |
| format | Article |
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To improve its capabilities for the heterologous expression of biosynthetic gene clusters, the complete genomic sequence of S. albus J1074 was obtained. With a size of 6,841,649 bp, coding for 5,832 genes, its genome is the smallest within the genus streptomycetes. Genome analysis revealed a strong tendency to reduce the number of genetic duplicates. The whole transcriptomes were sequenced at different time points to identify the early metabolic switch from the exponential to the stationary phase in S. albus J1074.
S. albus J1074 carries the smallest genome among the completely sequenced species of the genus Streptomyces. The detailed genome and transcriptome analysis discloses its capability to serve as a premium host for the heterologous production of natural products. Moreover, the genome revealed 22 additional putative secondary metabolite gene clusters that reinforce the strain's potential for natural product synthesis.</description><identifier>ISSN: 1471-2164</identifier><identifier>EISSN: 1471-2164</identifier><identifier>DOI: 10.1186/1471-2164-15-97</identifier><identifier>PMID: 24495463</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Anti-Bacterial Agents - pharmacology ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteriology ; Base Sequence ; Chromosomes ; Chromosomes, Bacterial - genetics ; Chromosomes, Bacterial - metabolism ; Drug discovery ; Drug Resistance, Bacterial - drug effects ; Drug Resistance, Bacterial - genetics ; Gene expression ; Genetic engineering ; Genome, Bacterial ; Genomes ; Genomics ; Metabolites ; Methods ; Molecular Sequence Data ; Multigene Family ; Proteins ; Sequence Analysis, RNA ; Streptomyces ; Streptomyces - genetics ; Streptomyces albus ; Streptomycetes</subject><ispartof>BMC genomics, 2014-02, Vol.15 (1), p.97-97, Article 97</ispartof><rights>COPYRIGHT 2014 BioMed Central Ltd.</rights><rights>2014 Zaburannyi et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.</rights><rights>Copyright © 2014 Zaburannyi et al.; licensee BioMed Central Ltd. 2014 Zaburannyi et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b614t-d9b984fc1068ce3d5d17ba32a905e9ca79d7eea269e18c84e81300e43a3e05693</citedby><cites>FETCH-LOGICAL-b614t-d9b984fc1068ce3d5d17ba32a905e9ca79d7eea269e18c84e81300e43a3e05693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3937824/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3937824/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24495463$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zaburannyi, Nestor</creatorcontrib><creatorcontrib>Rabyk, Mariia</creatorcontrib><creatorcontrib>Ostash, Bohdan</creatorcontrib><creatorcontrib>Fedorenko, Victor</creatorcontrib><creatorcontrib>Luzhetskyy, Andriy</creatorcontrib><title>Insights into naturally minimised Streptomyces albus J1074 genome</title><title>BMC genomics</title><addtitle>BMC Genomics</addtitle><description>The Streptomyces albus J1074 strain is one of the most widely used chassis for the heterologous production of bioactive natural products. The fast growth and an efficient genetic system make this strain an attractive model for expressing cryptic biosynthetic pathways to aid drug discovery.
To improve its capabilities for the heterologous expression of biosynthetic gene clusters, the complete genomic sequence of S. albus J1074 was obtained. With a size of 6,841,649 bp, coding for 5,832 genes, its genome is the smallest within the genus streptomycetes. Genome analysis revealed a strong tendency to reduce the number of genetic duplicates. The whole transcriptomes were sequenced at different time points to identify the early metabolic switch from the exponential to the stationary phase in S. albus J1074.
S. albus J1074 carries the smallest genome among the completely sequenced species of the genus Streptomyces. The detailed genome and transcriptome analysis discloses its capability to serve as a premium host for the heterologous production of natural products. Moreover, the genome revealed 22 additional putative secondary metabolite gene clusters that reinforce the strain's potential for natural product synthesis.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Chromosomes</subject><subject>Chromosomes, Bacterial - genetics</subject><subject>Chromosomes, Bacterial - metabolism</subject><subject>Drug discovery</subject><subject>Drug Resistance, Bacterial - drug effects</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Gene expression</subject><subject>Genetic engineering</subject><subject>Genome, Bacterial</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Metabolites</subject><subject>Methods</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Proteins</subject><subject>Sequence Analysis, RNA</subject><subject>Streptomyces</subject><subject>Streptomyces - 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Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC genomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zaburannyi, Nestor</au><au>Rabyk, Mariia</au><au>Ostash, Bohdan</au><au>Fedorenko, Victor</au><au>Luzhetskyy, Andriy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insights into naturally minimised Streptomyces albus J1074 genome</atitle><jtitle>BMC genomics</jtitle><addtitle>BMC Genomics</addtitle><date>2014-02-05</date><risdate>2014</risdate><volume>15</volume><issue>1</issue><spage>97</spage><epage>97</epage><pages>97-97</pages><artnum>97</artnum><issn>1471-2164</issn><eissn>1471-2164</eissn><abstract>The Streptomyces albus J1074 strain is one of the most widely used chassis for the heterologous production of bioactive natural products. The fast growth and an efficient genetic system make this strain an attractive model for expressing cryptic biosynthetic pathways to aid drug discovery.
To improve its capabilities for the heterologous expression of biosynthetic gene clusters, the complete genomic sequence of S. albus J1074 was obtained. With a size of 6,841,649 bp, coding for 5,832 genes, its genome is the smallest within the genus streptomycetes. Genome analysis revealed a strong tendency to reduce the number of genetic duplicates. The whole transcriptomes were sequenced at different time points to identify the early metabolic switch from the exponential to the stationary phase in S. albus J1074.
S. albus J1074 carries the smallest genome among the completely sequenced species of the genus Streptomyces. The detailed genome and transcriptome analysis discloses its capability to serve as a premium host for the heterologous production of natural products. Moreover, the genome revealed 22 additional putative secondary metabolite gene clusters that reinforce the strain's potential for natural product synthesis.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>24495463</pmid><doi>10.1186/1471-2164-15-97</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anti-Bacterial Agents - pharmacology Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Base Sequence Chromosomes Chromosomes, Bacterial - genetics Chromosomes, Bacterial - metabolism Drug discovery Drug Resistance, Bacterial - drug effects Drug Resistance, Bacterial - genetics Gene expression Genetic engineering Genome, Bacterial Genomes Genomics Metabolites Methods Molecular Sequence Data Multigene Family Proteins Sequence Analysis, RNA Streptomyces Streptomyces - genetics Streptomyces albus Streptomycetes |
| title | Insights into naturally minimised Streptomyces albus J1074 genome |
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