Validation and implementation of a method for microarray gene expression profiling of minor B-cell subpopulations in man

This report describes a method for the generation of global gene expression profiles from low frequent B-cell subsets by using fluorescence-activated cell sorting and RNA amplification. However, some of the differentiating compartments involve a low number of cells and therefore it is important to o...

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Veröffentlicht in:	BMC immunology 2014-01, Vol.15 (1), p.3-3, Article 3
Hauptverfasser:	Bergkvist, Kim Steve, Nyegaard, Mette, Bøgsted, Martin, Schmitz, Alexander, Bødker, Julie Støve, Rasmussen, Simon Mylius, Perez-Andres, Martin, Falgreen, Steffen, Bilgrau, Anders Ellern, Kjeldsen, Malene Krag, Gaihede, Michael, Nørgaard, Martin Agge, Bæch, John, Grønholdt, Marie-Louise, Jensen, Frank Svendsen, Johansen, Preben, Dybkær, Karen, Johnsen, Hans Erik
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Antigens, CD - metabolism B-Lymphocyte Subsets - metabolism Biological products industry Bone marrow Cancer Flow Cytometry Gene expression Gene Expression Profiling - methods Genetic aspects Hematology Humans Lymphoid Tissue - cytology Lymphoid Tissue - metabolism Medical equipment and supplies industry Medical research Medical test kit industry Messenger RNA Methods Oligonucleotide Array Sequence Analysis Organ Specificity - genetics Principal components analysis Real-Time Polymerase Chain Reaction Reproducibility of Results Statistical analysis Surgery Transcription factors Variance analysis
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