Plug-and-Play Fluorophores Extend the Spectral Properties of Spinach

Spinach and Spinach2 are RNA aptamers that can be used for the genetic encoding of fluorescent RNA. Spinach2 binds and activates the fluorescence of (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-1,2-dimethyl-1H-imidazol-5­(4H)-one (DFHBI), allowing the dynamic localizations of Spinach2-tagged RNAs to be...

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