Berberine, an isoquinoline alkaloid, inhibits melanoma cancer cell migration by reducing the expressions of cyclooxygenase-2, prostaglandin E2 and prostaglandin E2 receptors

Melanoma is the leading cause of death from skin disease due, in large part, to its propensity to metastasize. We have examined the effect of berberine, an isoquinoline alkaloid, on human melanoma cancer cell migration and the molecular mechanisms underlying these effects using melanoma cell lines,...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Carcinogenesis (New York) 2011-01, Vol.32 (1), p.86-92
Hauptverfasser: SINGH, Tripti, VAID, Mudit, KATIYAR, Nandan, SHARMA, Samriti, KATIYAR, Santosh K
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 92
container_issue 1
container_start_page 86
container_title Carcinogenesis (New York)
container_volume 32
creator SINGH, Tripti
VAID, Mudit
KATIYAR, Nandan
SHARMA, Samriti
KATIYAR, Santosh K
description Melanoma is the leading cause of death from skin disease due, in large part, to its propensity to metastasize. We have examined the effect of berberine, an isoquinoline alkaloid, on human melanoma cancer cell migration and the molecular mechanisms underlying these effects using melanoma cell lines, A375 and Hs294. Using an in vitro cell migration assay, we show that over expression of cyclooxygenase (COX)-2, its metabolite prostaglandin E₂ (PGE₂) and PGE₂ receptors promote the migration of cells. We found that treatment of A375 and Hs294 cells with berberine resulted in concentration-dependent inhibition of migration of these cells, which was associated with a reduction in the levels of COX-2, PGE₂ and PGE₂ receptors (EP2 and EP4). Treatment of cells with celecoxib, a COX-2 inhibitor, or transient transfection of cells with COX-2 small interfering RNA, also inhibited cell migration. Treatment of the cells with 12-O-tetradecanoylphorbol-13-acetate (TPA), an inducer of COX-2 or PGE₂, enhanced cell migration, whereas berberine inhibited TPA- or PGE₂-promoted cell migration. Berberine reduced the basal levels as well as PGE₂-stimulated expression levels of EP2 and EP4. Treatment of the cells with the EP4 agonist stimulated cell migration and berberine blocked EP4 agonist-induced cell migration activity. Moreover, berberine inhibited the activation of nuclear factor-kappa B (NF-κB), an upstream regulator of COX-2, in A375 cells, and treatment of cells with caffeic acid phenethyl ester, an inhibitor of NF-κB, inhibited cell migration. Together, these results indicate for the first time that berberine inhibits melanoma cell migration, an essential step in invasion and metastasis, by inhibition of COX-2, PGE₂ and PGE₂ receptors.
doi_str_mv 10.1093/carcin/bgq215
format Article
fullrecord <record><control><sourceid>pubmed_pasca</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3924040</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20974686</sourcerecordid><originalsourceid>FETCH-LOGICAL-p210t-5b77c921e54d2827faa3dedbbe3b6bd48021853f917f3f131a212f830c7827e93</originalsourceid><addsrcrecordid>eNplkEtvFDEQhC0URJbAkWvkS247xI95-YJEovCQInGB86ht98w6eOyJPRtlfxT_MZYIIYhTS1Vfl0pFyDvO3nOm5LmBZFw419Ot4M0LsuF1yyrBe3ZENozXspJS1sfkdc43jPFWNuoVORZMdXXbtxvy6wKTxuQCbikE6nK83bsQfREo-J_go7Nb6sLOabdmOqOHEGegBoLBRA16T2c3JVhdDFQfaEK7L4Umuu6Q4v2SMOdiZRpHag7Gx3h_mDBAxkps6ZJiXmEqodYFeiVKB_u_mNDgssaU35CXI_iMbx_vCfnx6er75Zfq-tvnr5cfr6tFcLZWje46owTHpraiF90IIC1arVHqVtu6Z2WgRo6Kd6McueQguBh7yUxXaFTyhHz4nbvs9YzWYFgT-GFJboZ0GCK44V8nuN0wxbtBKlGzmpWA0-cBT59_hi_A2SMA2YAfU9nT5b-c7DvFFZMPmheYvA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Berberine, an isoquinoline alkaloid, inhibits melanoma cancer cell migration by reducing the expressions of cyclooxygenase-2, prostaglandin E2 and prostaglandin E2 receptors</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>SINGH, Tripti ; VAID, Mudit ; KATIYAR, Nandan ; SHARMA, Samriti ; KATIYAR, Santosh K</creator><creatorcontrib>SINGH, Tripti ; VAID, Mudit ; KATIYAR, Nandan ; SHARMA, Samriti ; KATIYAR, Santosh K</creatorcontrib><description>Melanoma is the leading cause of death from skin disease due, in large part, to its propensity to metastasize. We have examined the effect of berberine, an isoquinoline alkaloid, on human melanoma cancer cell migration and the molecular mechanisms underlying these effects using melanoma cell lines, A375 and Hs294. Using an in vitro cell migration assay, we show that over expression of cyclooxygenase (COX)-2, its metabolite prostaglandin E₂ (PGE₂) and PGE₂ receptors promote the migration of cells. We found that treatment of A375 and Hs294 cells with berberine resulted in concentration-dependent inhibition of migration of these cells, which was associated with a reduction in the levels of COX-2, PGE₂ and PGE₂ receptors (EP2 and EP4). Treatment of cells with celecoxib, a COX-2 inhibitor, or transient transfection of cells with COX-2 small interfering RNA, also inhibited cell migration. Treatment of the cells with 12-O-tetradecanoylphorbol-13-acetate (TPA), an inducer of COX-2 or PGE₂, enhanced cell migration, whereas berberine inhibited TPA- or PGE₂-promoted cell migration. Berberine reduced the basal levels as well as PGE₂-stimulated expression levels of EP2 and EP4. Treatment of the cells with the EP4 agonist stimulated cell migration and berberine blocked EP4 agonist-induced cell migration activity. Moreover, berberine inhibited the activation of nuclear factor-kappa B (NF-κB), an upstream regulator of COX-2, in A375 cells, and treatment of cells with caffeic acid phenethyl ester, an inhibitor of NF-κB, inhibited cell migration. Together, these results indicate for the first time that berberine inhibits melanoma cell migration, an essential step in invasion and metastasis, by inhibition of COX-2, PGE₂ and PGE₂ receptors.</description><identifier>ISSN: 0143-3334</identifier><identifier>EISSN: 1460-2180</identifier><identifier>DOI: 10.1093/carcin/bgq215</identifier><identifier>PMID: 20974686</identifier><identifier>CODEN: CRNGDP</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Antineoplastic Agents - pharmacology ; Berberine - pharmacology ; Biological and medical sciences ; Blotting, Western ; Cancer Prevention ; Carcinogenesis, carcinogens and anticarcinogens ; Cell Line, Tumor ; Cell Movement - drug effects ; Cyclooxygenase 2 - biosynthesis ; Cyclooxygenase 2 - drug effects ; Dermatology ; Dinoprostone - biosynthesis ; Gene Expression - drug effects ; Humans ; Medical sciences ; Melanoma - genetics ; Melanoma - metabolism ; Receptors, Prostaglandin E - biosynthesis ; Receptors, Prostaglandin E - drug effects ; Transfection ; Tumors ; Tumors of the skin and soft tissue. Premalignant lesions</subject><ispartof>Carcinogenesis (New York), 2011-01, Vol.32 (1), p.86-92</ispartof><rights>2015 INIST-CNRS</rights><rights>The Author 2010. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=23879190$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20974686$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SINGH, Tripti</creatorcontrib><creatorcontrib>VAID, Mudit</creatorcontrib><creatorcontrib>KATIYAR, Nandan</creatorcontrib><creatorcontrib>SHARMA, Samriti</creatorcontrib><creatorcontrib>KATIYAR, Santosh K</creatorcontrib><title>Berberine, an isoquinoline alkaloid, inhibits melanoma cancer cell migration by reducing the expressions of cyclooxygenase-2, prostaglandin E2 and prostaglandin E2 receptors</title><title>Carcinogenesis (New York)</title><addtitle>Carcinogenesis</addtitle><description>Melanoma is the leading cause of death from skin disease due, in large part, to its propensity to metastasize. We have examined the effect of berberine, an isoquinoline alkaloid, on human melanoma cancer cell migration and the molecular mechanisms underlying these effects using melanoma cell lines, A375 and Hs294. Using an in vitro cell migration assay, we show that over expression of cyclooxygenase (COX)-2, its metabolite prostaglandin E₂ (PGE₂) and PGE₂ receptors promote the migration of cells. We found that treatment of A375 and Hs294 cells with berberine resulted in concentration-dependent inhibition of migration of these cells, which was associated with a reduction in the levels of COX-2, PGE₂ and PGE₂ receptors (EP2 and EP4). Treatment of cells with celecoxib, a COX-2 inhibitor, or transient transfection of cells with COX-2 small interfering RNA, also inhibited cell migration. Treatment of the cells with 12-O-tetradecanoylphorbol-13-acetate (TPA), an inducer of COX-2 or PGE₂, enhanced cell migration, whereas berberine inhibited TPA- or PGE₂-promoted cell migration. Berberine reduced the basal levels as well as PGE₂-stimulated expression levels of EP2 and EP4. Treatment of the cells with the EP4 agonist stimulated cell migration and berberine blocked EP4 agonist-induced cell migration activity. Moreover, berberine inhibited the activation of nuclear factor-kappa B (NF-κB), an upstream regulator of COX-2, in A375 cells, and treatment of cells with caffeic acid phenethyl ester, an inhibitor of NF-κB, inhibited cell migration. Together, these results indicate for the first time that berberine inhibits melanoma cell migration, an essential step in invasion and metastasis, by inhibition of COX-2, PGE₂ and PGE₂ receptors.</description><subject>Antineoplastic Agents - pharmacology</subject><subject>Berberine - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cancer Prevention</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - drug effects</subject><subject>Cyclooxygenase 2 - biosynthesis</subject><subject>Cyclooxygenase 2 - drug effects</subject><subject>Dermatology</subject><subject>Dinoprostone - biosynthesis</subject><subject>Gene Expression - drug effects</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Melanoma - genetics</subject><subject>Melanoma - metabolism</subject><subject>Receptors, Prostaglandin E - biosynthesis</subject><subject>Receptors, Prostaglandin E - drug effects</subject><subject>Transfection</subject><subject>Tumors</subject><subject>Tumors of the skin and soft tissue. Premalignant lesions</subject><issn>0143-3334</issn><issn>1460-2180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkEtvFDEQhC0URJbAkWvkS247xI95-YJEovCQInGB86ht98w6eOyJPRtlfxT_MZYIIYhTS1Vfl0pFyDvO3nOm5LmBZFw419Ot4M0LsuF1yyrBe3ZENozXspJS1sfkdc43jPFWNuoVORZMdXXbtxvy6wKTxuQCbikE6nK83bsQfREo-J_go7Nb6sLOabdmOqOHEGegBoLBRA16T2c3JVhdDFQfaEK7L4Umuu6Q4v2SMOdiZRpHag7Gx3h_mDBAxkps6ZJiXmEqodYFeiVKB_u_mNDgssaU35CXI_iMbx_vCfnx6er75Zfq-tvnr5cfr6tFcLZWje46owTHpraiF90IIC1arVHqVtu6Z2WgRo6Kd6McueQguBh7yUxXaFTyhHz4nbvs9YzWYFgT-GFJboZ0GCK44V8nuN0wxbtBKlGzmpWA0-cBT59_hi_A2SMA2YAfU9nT5b-c7DvFFZMPmheYvA</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>SINGH, Tripti</creator><creator>VAID, Mudit</creator><creator>KATIYAR, Nandan</creator><creator>SHARMA, Samriti</creator><creator>KATIYAR, Santosh K</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>5PM</scope></search><sort><creationdate>20110101</creationdate><title>Berberine, an isoquinoline alkaloid, inhibits melanoma cancer cell migration by reducing the expressions of cyclooxygenase-2, prostaglandin E2 and prostaglandin E2 receptors</title><author>SINGH, Tripti ; VAID, Mudit ; KATIYAR, Nandan ; SHARMA, Samriti ; KATIYAR, Santosh K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p210t-5b77c921e54d2827faa3dedbbe3b6bd48021853f917f3f131a212f830c7827e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Antineoplastic Agents - pharmacology</topic><topic>Berberine - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cancer Prevention</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - drug effects</topic><topic>Cyclooxygenase 2 - biosynthesis</topic><topic>Cyclooxygenase 2 - drug effects</topic><topic>Dermatology</topic><topic>Dinoprostone - biosynthesis</topic><topic>Gene Expression - drug effects</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Melanoma - genetics</topic><topic>Melanoma - metabolism</topic><topic>Receptors, Prostaglandin E - biosynthesis</topic><topic>Receptors, Prostaglandin E - drug effects</topic><topic>Transfection</topic><topic>Tumors</topic><topic>Tumors of the skin and soft tissue. Premalignant lesions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SINGH, Tripti</creatorcontrib><creatorcontrib>VAID, Mudit</creatorcontrib><creatorcontrib>KATIYAR, Nandan</creatorcontrib><creatorcontrib>SHARMA, Samriti</creatorcontrib><creatorcontrib>KATIYAR, Santosh K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Carcinogenesis (New York)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SINGH, Tripti</au><au>VAID, Mudit</au><au>KATIYAR, Nandan</au><au>SHARMA, Samriti</au><au>KATIYAR, Santosh K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Berberine, an isoquinoline alkaloid, inhibits melanoma cancer cell migration by reducing the expressions of cyclooxygenase-2, prostaglandin E2 and prostaglandin E2 receptors</atitle><jtitle>Carcinogenesis (New York)</jtitle><addtitle>Carcinogenesis</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>32</volume><issue>1</issue><spage>86</spage><epage>92</epage><pages>86-92</pages><issn>0143-3334</issn><eissn>1460-2180</eissn><coden>CRNGDP</coden><abstract>Melanoma is the leading cause of death from skin disease due, in large part, to its propensity to metastasize. We have examined the effect of berberine, an isoquinoline alkaloid, on human melanoma cancer cell migration and the molecular mechanisms underlying these effects using melanoma cell lines, A375 and Hs294. Using an in vitro cell migration assay, we show that over expression of cyclooxygenase (COX)-2, its metabolite prostaglandin E₂ (PGE₂) and PGE₂ receptors promote the migration of cells. We found that treatment of A375 and Hs294 cells with berberine resulted in concentration-dependent inhibition of migration of these cells, which was associated with a reduction in the levels of COX-2, PGE₂ and PGE₂ receptors (EP2 and EP4). Treatment of cells with celecoxib, a COX-2 inhibitor, or transient transfection of cells with COX-2 small interfering RNA, also inhibited cell migration. Treatment of the cells with 12-O-tetradecanoylphorbol-13-acetate (TPA), an inducer of COX-2 or PGE₂, enhanced cell migration, whereas berberine inhibited TPA- or PGE₂-promoted cell migration. Berberine reduced the basal levels as well as PGE₂-stimulated expression levels of EP2 and EP4. Treatment of the cells with the EP4 agonist stimulated cell migration and berberine blocked EP4 agonist-induced cell migration activity. Moreover, berberine inhibited the activation of nuclear factor-kappa B (NF-κB), an upstream regulator of COX-2, in A375 cells, and treatment of cells with caffeic acid phenethyl ester, an inhibitor of NF-κB, inhibited cell migration. Together, these results indicate for the first time that berberine inhibits melanoma cell migration, an essential step in invasion and metastasis, by inhibition of COX-2, PGE₂ and PGE₂ receptors.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>20974686</pmid><doi>10.1093/carcin/bgq215</doi><tpages>7</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0143-3334
ispartof Carcinogenesis (New York), 2011-01, Vol.32 (1), p.86-92
issn 0143-3334
1460-2180
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3924040
source MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Antineoplastic Agents - pharmacology
Berberine - pharmacology
Biological and medical sciences
Blotting, Western
Cancer Prevention
Carcinogenesis, carcinogens and anticarcinogens
Cell Line, Tumor
Cell Movement - drug effects
Cyclooxygenase 2 - biosynthesis
Cyclooxygenase 2 - drug effects
Dermatology
Dinoprostone - biosynthesis
Gene Expression - drug effects
Humans
Medical sciences
Melanoma - genetics
Melanoma - metabolism
Receptors, Prostaglandin E - biosynthesis
Receptors, Prostaglandin E - drug effects
Transfection
Tumors
Tumors of the skin and soft tissue. Premalignant lesions
title Berberine, an isoquinoline alkaloid, inhibits melanoma cancer cell migration by reducing the expressions of cyclooxygenase-2, prostaglandin E2 and prostaglandin E2 receptors
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T02%3A04%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Berberine,%20an%20isoquinoline%20alkaloid,%20inhibits%20melanoma%20cancer%20cell%20migration%20by%20reducing%20the%20expressions%20of%20cyclooxygenase-2,%20prostaglandin%20E2%20and%20prostaglandin%20E2%20receptors&rft.jtitle=Carcinogenesis%20(New%20York)&rft.au=SINGH,%20Tripti&rft.date=2011-01-01&rft.volume=32&rft.issue=1&rft.spage=86&rft.epage=92&rft.pages=86-92&rft.issn=0143-3334&rft.eissn=1460-2180&rft.coden=CRNGDP&rft_id=info:doi/10.1093/carcin/bgq215&rft_dat=%3Cpubmed_pasca%3E20974686%3C/pubmed_pasca%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/20974686&rfr_iscdi=true