Fast fixing and comprehensive identification to help improve real-time ligands discovery based on formaldehyde crosslinking, immunoprecipitation and SDS-PAGE separation

Fast Fixation is necessary to study real-time protein-protein interactions under physiological conditions. Fast formaldehyde cross-linking can fix transient and weak protein interactions, thereby reducing the number of false negatives but producing great complexity. To reduce this complexity, immuno...

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Veröffentlicht in:	Proteome science 2014-02, Vol.12 (1), p.6-6, Article 6
Hauptverfasser:	Zhu, Lisi, Li, Menglin, Wei, Lilong, Liu, Xuejiao, Yin, Jianrui, Gao, Youhe
Format:	Artikel
Sprache:	eng
Schlagworte:	Albumin Analysis Antibodies Chemical bonds Experiments Ligands Mass spectrometry Molecular weight Physiological aspects Protein-protein interactions Proteins Science Viral antibodies
Online-Zugang:	Volltext
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