Optical axial scanning in confocal microscopy using an electrically tunable lens

This paper presents the use and characterization of an electrically focus tunable lens to perform axial scanning in a confocal microscope. Lateral and axial resolution are characterized over a >250 µm axial scan range. Confocal microscopy using optical axial scanning is demonstrated in epithelial...

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Veröffentlicht in:	Biomedical optics express 2014-02, Vol.5 (2), p.645-652
Hauptverfasser:	Jabbour, Joey M, Malik, Bilal H, Olsovsky, Cory, Cuenca, Rodrigo, Cheng, Shuna, Jo, Javier A, Cheng, Yi-Shing Lisa, Wright, John M, Maitland, Kristen C
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Sprache:	eng
Schlagworte:	Microscopy
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creator	Jabbour, Joey M Malik, Bilal H Olsovsky, Cory Cuenca, Rodrigo Cheng, Shuna Jo, Javier A Cheng, Yi-Shing Lisa Wright, John M Maitland, Kristen C
description	This paper presents the use and characterization of an electrically focus tunable lens to perform axial scanning in a confocal microscope. Lateral and axial resolution are characterized over a >250 µm axial scan range. Confocal microscopy using optical axial scanning is demonstrated in epithelial tissue and compared to traditional stage scanning. By enabling rapid axial scanning, minimizing motion artifacts, and reducing mechanical complexity, this technique has potential to enhance in vivo three-dimensional imaging in confocal endomicroscopy.
doi_str_mv	10.1364/BOE.5.000645
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subjects	Microscopy
title	Optical axial scanning in confocal microscopy using an electrically tunable lens
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