Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach

In Latin America, Lutzomyia longipalpis is the main vector of the protozoan parasite Leishmania infantum, which is the causal agent of American Visceral Leishmaniasis. This insect uses male-produced pheromones for mate recognition. Elucidation of pheromone biogenesis or its regulation may enable mol...

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Veröffentlicht in:Journal of proteomics 2014-01, Vol.96, p.117-132
Hauptverfasser: González-Caballero, Natalia, Rodríguez-Vega, Andrés, Dias-Lopes, Geovane, Valenzuela, Jesus G., Ribeiro, Jose M.C., Carvalho, Paulo Costa, Valente, Richard H., Brazil, Reginaldo P., Cuervo, Patricia
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container_title Journal of proteomics
container_volume 96
creator González-Caballero, Natalia
Rodríguez-Vega, Andrés
Dias-Lopes, Geovane
Valenzuela, Jesus G.
Ribeiro, Jose M.C.
Carvalho, Paulo Costa
Valente, Richard H.
Brazil, Reginaldo P.
Cuervo, Patricia
description In Latin America, Lutzomyia longipalpis is the main vector of the protozoan parasite Leishmania infantum, which is the causal agent of American Visceral Leishmaniasis. This insect uses male-produced pheromones for mate recognition. Elucidation of pheromone biogenesis or its regulation may enable molecular strategies for mating disruption and, consequently, the vector's population management. Motivated by our recent results of the transcriptomic characterization of the L. longipalpis pheromone gland, we performed a proteomic analysis of this tissue combining SDS-PAGE, and mass spectrometry followed by an integrative data analysis. Considering that annotated genome sequences of this sand fly are not available, we designed an alternative workflow searching MS/MS data against two customized databases using three search engines: Mascot, OMSSA and ProLuCID. A total of 542 proteins were confidently characterized, 445 of them using a Uniref100-insect protein database, and 97 using a transcript translated database. In addition, use of PEAKS for de novo peptide sequencing of MS/MS data confirmed ~90% identifications made with the combination of the three search engines. Our results include the identification of six of the seven enzymes of the mevalonate-pathway, plus the enzymes involved in sesquiterpenoid biosynthesis, all of which are proposed to be involved in pheromone production in L. longipalpis. L. longipalpis is the main vector of the protozoan parasite L. infantum, which is the causal agent of American Visceral Leishmaniasis. One of the control measures of such disease is focused on vector population control. As this insect uses male-produced pheromones for mate recognition, the elucidation of pheromone biogenesis or its regulating process may enable molecular strategies for mating disruption and, consequently, this vector's population management. On this regard, in this manuscript we report expression evidence, at the protein level, of several molecules potentially involved in the pheromone production of L. longipalpis. Our results include the identification of the mevalonate-pathway enzymes, plus the enzymes involved in sesquiterpenoid biosynthesis, all of which are proposed to be involved in pheromone production in L. longipalpis. In addition, considering that the annotated genome sequences of this sand fly are not yet available, we designed an alternative workflow searching MS/MS data against proteomic and transcript translated customized databases, usi
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This insect uses male-produced pheromones for mate recognition. Elucidation of pheromone biogenesis or its regulation may enable molecular strategies for mating disruption and, consequently, the vector's population management. Motivated by our recent results of the transcriptomic characterization of the L. longipalpis pheromone gland, we performed a proteomic analysis of this tissue combining SDS-PAGE, and mass spectrometry followed by an integrative data analysis. Considering that annotated genome sequences of this sand fly are not available, we designed an alternative workflow searching MS/MS data against two customized databases using three search engines: Mascot, OMSSA and ProLuCID. A total of 542 proteins were confidently characterized, 445 of them using a Uniref100-insect protein database, and 97 using a transcript translated database. In addition, use of PEAKS for de novo peptide sequencing of MS/MS data confirmed ~90% identifications made with the combination of the three search engines. Our results include the identification of six of the seven enzymes of the mevalonate-pathway, plus the enzymes involved in sesquiterpenoid biosynthesis, all of which are proposed to be involved in pheromone production in L. longipalpis. L. longipalpis is the main vector of the protozoan parasite L. infantum, which is the causal agent of American Visceral Leishmaniasis. One of the control measures of such disease is focused on vector population control. As this insect uses male-produced pheromones for mate recognition, the elucidation of pheromone biogenesis or its regulating process may enable molecular strategies for mating disruption and, consequently, this vector's population management. On this regard, in this manuscript we report expression evidence, at the protein level, of several molecules potentially involved in the pheromone production of L. longipalpis. Our results include the identification of the mevalonate-pathway enzymes, plus the enzymes involved in sesquiterpenoid biosynthesis, all of which are proposed to be involved in pheromone production in L. longipalpis. In addition, considering that the annotated genome sequences of this sand fly are not yet available, we designed an alternative workflow searching MS/MS data against proteomic and transcript translated customized databases, using three search engines: Mascot, OMSSA, and ProLuCID. In addition, a de novo peptide sequencing software (PEAKS) was used to further analyze the MS/MS data. This approach made it possible to identify and annotate 542 proteins for the pheromone gland of L. longipalpis. Importantly, all annotated protein sequences and raw data are available for the research community in protein repositories that provide free access to the data. [Display omitted] •Lutzomyia longipalpis is the main vector of Leishmania infantum in the Americas.•Elucidation of pheromone biogenesis may enable molecular strategies for mating disruption and population management.•A proteomic and transcriptomic approach allowed in-depth characterization of the sandfly pheromone gland.•Enzymes of the mevalonate pathway and sesquiterpenoid biosynthesis are proposed to be involved in pheromone production.</description><identifier>ISSN: 1874-3919</identifier><identifier>EISSN: 1876-7737</identifier><identifier>DOI: 10.1016/j.jprot.2013.10.028</identifier><identifier>PMID: 24185139</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Diptera ; Diptera - enzymology ; Diptera - genetics ; Diptera - parasitology ; Exocrine Glands - enzymology ; Female ; Gene Expression Regulation, Enzymologic ; Insect Proteins - biosynthesis ; Insect Proteins - genetics ; Insect Vectors - enzymology ; Insect Vectors - genetics ; Insect Vectors - parasitology ; Leishmania infantum ; Leishmaniasis, Visceral ; Lutzomyia longipalpis ; Male ; Male pheromone gland ; Mass Spectrometry ; Mevalonate pathway ; Mevalonic Acid - metabolism ; Proteome ; Proteomics ; Psychodidae ; Sex Attractants - genetics ; Sex Attractants - metabolism</subject><ispartof>Journal of proteomics, 2014-01, Vol.96, p.117-132</ispartof><rights>2013 Elsevier B.V.</rights><rights>2013.</rights><rights>2013 Elsevier B.V. All rights reserved. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c492t-f93747037a739f8fd4008a581a5cc6ab2882783a1e06b62a84edebaf6d416b113</citedby><cites>FETCH-LOGICAL-c492t-f93747037a739f8fd4008a581a5cc6ab2882783a1e06b62a84edebaf6d416b113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jprot.2013.10.028$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24185139$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>González-Caballero, Natalia</creatorcontrib><creatorcontrib>Rodríguez-Vega, Andrés</creatorcontrib><creatorcontrib>Dias-Lopes, Geovane</creatorcontrib><creatorcontrib>Valenzuela, Jesus G.</creatorcontrib><creatorcontrib>Ribeiro, Jose M.C.</creatorcontrib><creatorcontrib>Carvalho, Paulo Costa</creatorcontrib><creatorcontrib>Valente, Richard H.</creatorcontrib><creatorcontrib>Brazil, Reginaldo P.</creatorcontrib><creatorcontrib>Cuervo, Patricia</creatorcontrib><title>Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach</title><title>Journal of proteomics</title><addtitle>J Proteomics</addtitle><description>In Latin America, Lutzomyia longipalpis is the main vector of the protozoan parasite Leishmania infantum, which is the causal agent of American Visceral Leishmaniasis. This insect uses male-produced pheromones for mate recognition. Elucidation of pheromone biogenesis or its regulation may enable molecular strategies for mating disruption and, consequently, the vector's population management. Motivated by our recent results of the transcriptomic characterization of the L. longipalpis pheromone gland, we performed a proteomic analysis of this tissue combining SDS-PAGE, and mass spectrometry followed by an integrative data analysis. Considering that annotated genome sequences of this sand fly are not available, we designed an alternative workflow searching MS/MS data against two customized databases using three search engines: Mascot, OMSSA and ProLuCID. A total of 542 proteins were confidently characterized, 445 of them using a Uniref100-insect protein database, and 97 using a transcript translated database. In addition, use of PEAKS for de novo peptide sequencing of MS/MS data confirmed ~90% identifications made with the combination of the three search engines. Our results include the identification of six of the seven enzymes of the mevalonate-pathway, plus the enzymes involved in sesquiterpenoid biosynthesis, all of which are proposed to be involved in pheromone production in L. longipalpis. L. longipalpis is the main vector of the protozoan parasite L. infantum, which is the causal agent of American Visceral Leishmaniasis. One of the control measures of such disease is focused on vector population control. As this insect uses male-produced pheromones for mate recognition, the elucidation of pheromone biogenesis or its regulating process may enable molecular strategies for mating disruption and, consequently, this vector's population management. On this regard, in this manuscript we report expression evidence, at the protein level, of several molecules potentially involved in the pheromone production of L. longipalpis. Our results include the identification of the mevalonate-pathway enzymes, plus the enzymes involved in sesquiterpenoid biosynthesis, all of which are proposed to be involved in pheromone production in L. longipalpis. In addition, considering that the annotated genome sequences of this sand fly are not yet available, we designed an alternative workflow searching MS/MS data against proteomic and transcript translated customized databases, using three search engines: Mascot, OMSSA, and ProLuCID. In addition, a de novo peptide sequencing software (PEAKS) was used to further analyze the MS/MS data. This approach made it possible to identify and annotate 542 proteins for the pheromone gland of L. longipalpis. Importantly, all annotated protein sequences and raw data are available for the research community in protein repositories that provide free access to the data. [Display omitted] •Lutzomyia longipalpis is the main vector of Leishmania infantum in the Americas.•Elucidation of pheromone biogenesis may enable molecular strategies for mating disruption and population management.•A proteomic and transcriptomic approach allowed in-depth characterization of the sandfly pheromone gland.•Enzymes of the mevalonate pathway and sesquiterpenoid biosynthesis are proposed to be involved in pheromone production.</description><subject>Animals</subject><subject>Diptera</subject><subject>Diptera - enzymology</subject><subject>Diptera - genetics</subject><subject>Diptera - parasitology</subject><subject>Exocrine Glands - enzymology</subject><subject>Female</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Insect Proteins - biosynthesis</subject><subject>Insect Proteins - genetics</subject><subject>Insect Vectors - enzymology</subject><subject>Insect Vectors - genetics</subject><subject>Insect Vectors - parasitology</subject><subject>Leishmania infantum</subject><subject>Leishmaniasis, Visceral</subject><subject>Lutzomyia longipalpis</subject><subject>Male</subject><subject>Male pheromone gland</subject><subject>Mass Spectrometry</subject><subject>Mevalonate pathway</subject><subject>Mevalonic Acid - metabolism</subject><subject>Proteome</subject><subject>Proteomics</subject><subject>Psychodidae</subject><subject>Sex Attractants - genetics</subject><subject>Sex Attractants - metabolism</subject><issn>1874-3919</issn><issn>1876-7737</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1v1DAQjRCIlsIvQEI-lkMWO07sBAkkVMqHtBIc4GxN7MnGqyQOtndp-n_4n3h3SwUXTh7PvHlvZl6WPWd0xSgTr7ar7exdXBWU8ZRZ0aJ-kJ2zWopcSi4fHuMy5w1rzrInIWwpFUw28nF2VpSsrhhvzrNf1zezxxCsm4jrSOyRjLiHwU0QkcwQ-5-wEJxulxEDsdMRsd7FWzcuFkjCbewMw2wDuXxv54geXpOvYdG9M9YAviQBb8jco3ejm5BsBpgMMZg-IfqkYUi7EJgSdcTNKXHYCt1oNYE5xaD7p9mjDoaAz-7ei-z7h-tvV5_y9ZePn6_erXNdNkXMu4bLUlIuQfKmqztTUlpDVTOotBbQFnVdyJoDQypaUUBdosEWOmFKJlrG-EX29sQ779oRjcYpzTio2dsR_KIcWPVvZbK92ri9SkeWlSgSweUdgXc_dhiiGm3QOKSt0e2CYpJXTbo-FwnKT1DtXQgeu3sZRtXBYLVVR4PVweBDMhmcul78PeF9zx9HE-DNCYDpTnuLXgVtcdJorEcdlXH2vwK_AfSOvjo</recordid><startdate>20140116</startdate><enddate>20140116</enddate><creator>González-Caballero, Natalia</creator><creator>Rodríguez-Vega, Andrés</creator><creator>Dias-Lopes, Geovane</creator><creator>Valenzuela, Jesus G.</creator><creator>Ribeiro, Jose M.C.</creator><creator>Carvalho, Paulo Costa</creator><creator>Valente, Richard H.</creator><creator>Brazil, Reginaldo P.</creator><creator>Cuervo, Patricia</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>5PM</scope></search><sort><creationdate>20140116</creationdate><title>Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach</title><author>González-Caballero, Natalia ; Rodríguez-Vega, Andrés ; Dias-Lopes, Geovane ; Valenzuela, Jesus G. ; Ribeiro, Jose M.C. ; Carvalho, Paulo Costa ; Valente, Richard H. ; Brazil, Reginaldo P. ; Cuervo, Patricia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-f93747037a739f8fd4008a581a5cc6ab2882783a1e06b62a84edebaf6d416b113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Diptera</topic><topic>Diptera - enzymology</topic><topic>Diptera - genetics</topic><topic>Diptera - parasitology</topic><topic>Exocrine Glands - enzymology</topic><topic>Female</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Insect Proteins - biosynthesis</topic><topic>Insect Proteins - genetics</topic><topic>Insect Vectors - enzymology</topic><topic>Insect Vectors - genetics</topic><topic>Insect Vectors - parasitology</topic><topic>Leishmania infantum</topic><topic>Leishmaniasis, Visceral</topic><topic>Lutzomyia longipalpis</topic><topic>Male</topic><topic>Male pheromone gland</topic><topic>Mass Spectrometry</topic><topic>Mevalonate pathway</topic><topic>Mevalonic Acid - metabolism</topic><topic>Proteome</topic><topic>Proteomics</topic><topic>Psychodidae</topic><topic>Sex Attractants - genetics</topic><topic>Sex Attractants - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>González-Caballero, Natalia</creatorcontrib><creatorcontrib>Rodríguez-Vega, Andrés</creatorcontrib><creatorcontrib>Dias-Lopes, Geovane</creatorcontrib><creatorcontrib>Valenzuela, Jesus G.</creatorcontrib><creatorcontrib>Ribeiro, Jose M.C.</creatorcontrib><creatorcontrib>Carvalho, Paulo Costa</creatorcontrib><creatorcontrib>Valente, Richard H.</creatorcontrib><creatorcontrib>Brazil, Reginaldo P.</creatorcontrib><creatorcontrib>Cuervo, Patricia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of proteomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>González-Caballero, Natalia</au><au>Rodríguez-Vega, Andrés</au><au>Dias-Lopes, Geovane</au><au>Valenzuela, Jesus G.</au><au>Ribeiro, Jose M.C.</au><au>Carvalho, Paulo Costa</au><au>Valente, Richard H.</au><au>Brazil, Reginaldo P.</au><au>Cuervo, Patricia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach</atitle><jtitle>Journal of proteomics</jtitle><addtitle>J Proteomics</addtitle><date>2014-01-16</date><risdate>2014</risdate><volume>96</volume><spage>117</spage><epage>132</epage><pages>117-132</pages><issn>1874-3919</issn><eissn>1876-7737</eissn><abstract>In Latin America, Lutzomyia longipalpis is the main vector of the protozoan parasite Leishmania infantum, which is the causal agent of American Visceral Leishmaniasis. This insect uses male-produced pheromones for mate recognition. Elucidation of pheromone biogenesis or its regulation may enable molecular strategies for mating disruption and, consequently, the vector's population management. Motivated by our recent results of the transcriptomic characterization of the L. longipalpis pheromone gland, we performed a proteomic analysis of this tissue combining SDS-PAGE, and mass spectrometry followed by an integrative data analysis. Considering that annotated genome sequences of this sand fly are not available, we designed an alternative workflow searching MS/MS data against two customized databases using three search engines: Mascot, OMSSA and ProLuCID. A total of 542 proteins were confidently characterized, 445 of them using a Uniref100-insect protein database, and 97 using a transcript translated database. In addition, use of PEAKS for de novo peptide sequencing of MS/MS data confirmed ~90% identifications made with the combination of the three search engines. Our results include the identification of six of the seven enzymes of the mevalonate-pathway, plus the enzymes involved in sesquiterpenoid biosynthesis, all of which are proposed to be involved in pheromone production in L. longipalpis. L. longipalpis is the main vector of the protozoan parasite L. infantum, which is the causal agent of American Visceral Leishmaniasis. One of the control measures of such disease is focused on vector population control. As this insect uses male-produced pheromones for mate recognition, the elucidation of pheromone biogenesis or its regulating process may enable molecular strategies for mating disruption and, consequently, this vector's population management. On this regard, in this manuscript we report expression evidence, at the protein level, of several molecules potentially involved in the pheromone production of L. longipalpis. Our results include the identification of the mevalonate-pathway enzymes, plus the enzymes involved in sesquiterpenoid biosynthesis, all of which are proposed to be involved in pheromone production in L. longipalpis. In addition, considering that the annotated genome sequences of this sand fly are not yet available, we designed an alternative workflow searching MS/MS data against proteomic and transcript translated customized databases, using three search engines: Mascot, OMSSA, and ProLuCID. In addition, a de novo peptide sequencing software (PEAKS) was used to further analyze the MS/MS data. This approach made it possible to identify and annotate 542 proteins for the pheromone gland of L. longipalpis. Importantly, all annotated protein sequences and raw data are available for the research community in protein repositories that provide free access to the data. [Display omitted] •Lutzomyia longipalpis is the main vector of Leishmania infantum in the Americas.•Elucidation of pheromone biogenesis may enable molecular strategies for mating disruption and population management.•A proteomic and transcriptomic approach allowed in-depth characterization of the sandfly pheromone gland.•Enzymes of the mevalonate pathway and sesquiterpenoid biosynthesis are proposed to be involved in pheromone production.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>24185139</pmid><doi>10.1016/j.jprot.2013.10.028</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record>
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source MEDLINE; ScienceDirect Journals (5 years ago - present)
subjects Animals
Diptera
Diptera - enzymology
Diptera - genetics
Diptera - parasitology
Exocrine Glands - enzymology
Female
Gene Expression Regulation, Enzymologic
Insect Proteins - biosynthesis
Insect Proteins - genetics
Insect Vectors - enzymology
Insect Vectors - genetics
Insect Vectors - parasitology
Leishmania infantum
Leishmaniasis, Visceral
Lutzomyia longipalpis
Male
Male pheromone gland
Mass Spectrometry
Mevalonate pathway
Mevalonic Acid - metabolism
Proteome
Proteomics
Psychodidae
Sex Attractants - genetics
Sex Attractants - metabolism
title Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach
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