Using phage display selected antibodies to dissect microbiomes for complete de novo genome sequencing of low abundance microbes

Single cell genomics has revolutionized microbial sequencing, but complete coverage of genomes in complex microbiomes is imperfect due to enormous variation in organismal abundance and amplification bias. Empirical methods that complement rapidly improving bioinformatic tools will improve characteri...

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Veröffentlicht in:	BMC microbiology 2013-11, Vol.13 (1), p.270-270, Article 270
Hauptverfasser:	Close, Devin W, Ferrara, Fortunato, Dichosa, Armand E K, Kumar, Sandeep, Daughton, Ashlynn R, Daligault, Hajnalka E, Reitenga, Krista G, Velappan, Nileena, Sanchez, Timothy C, Iyer, Srinivas, Kiss, Csaba, Han, Cliff S, Bradbury, Andrew R M
Format:	Artikel
Sprache:	eng
Schlagworte:	Abundance Antibodies Antibodies, Bacterial - immunology Antibodies, Bacterial - isolation & purification Antibodies, Bacterial - metabolism Bacteria Bacterial Load - methods BASIC BIOLOGICAL SCIENCES Bias Cell Surface Display Techniques DNA sequencing Earthquakes Flow cytometry Flow Cytometry - methods Genetic aspects Genome completion Genomes Lactobacillus acidophilus Lactobacillus acidophilus - genetics Lactobacillus acidophilus - immunology Lactobacillus acidophilus - isolation & purification MDA Medical equipment and supplies industry Medical test kit industry Methodology Methods Microbial genetics Microbiology Microbiota Molecular Sequence Data Molecular weight Nucleotide sequencing Phage antibodies Proteins Sequence Analysis, DNA - methods Single cell genomics Single-Chain Antibodies - immunology Single-Chain Antibodies - isolation & purification Single-Chain Antibodies - metabolism Software Viral antibodies
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