Detection of clonal antigen receptor gene rearrangement in dogs with lymphoma by real-time polymerase chain reaction and melting curve analysis

Molecular techniques that detect canine lymphoma cells by their clonal antigen receptor gene rearrangement play an increasing role for diagnosis as well as for monitoring minimal residual disease during and after cytostatic therapy. However, the methods currently available are time-consuming and/or...

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Veröffentlicht in:	BMC veterinary research 2014-01, Vol.10 (1), p.1-1
Hauptverfasser:	Langner, Kathrin F A, Joetzke, Alexa E, Nerschbach, Verena, Eberle, Nina, Schuberth, Hans-Joachim, Koy, Mirja, Nolte, Ingo, Betz, Daniela
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals antigens Cellular biology Chemotherapy Colleges & universities Deoxyribonucleic acid detection limit DNA Dog Diseases - genetics Dog Diseases - metabolism Dogs Flow cytometry Gene Expression Regulation, Neoplastic - physiology genes Genetic aspects Genetic research hyperplasia lymph nodes Lymphocytes Lymphoma Lymphoma, B-Cell - genetics Lymphoma, B-Cell - metabolism Lymphoma, B-Cell - veterinary Lymphoma, T-Cell - genetics Lymphoma, T-Cell - metabolism Lymphoma, T-Cell - veterinary melting Methodology Methods monitoring neoplasm cells Nucleic Acid Denaturation patients polyacrylamide gel electrophoresis Polymerase chain reaction quantitative polymerase chain reaction Real-Time Polymerase Chain Reaction - methods Real-Time Polymerase Chain Reaction - veterinary Receptors, Antigen - genetics Receptors, Antigen - metabolism T cell receptors T-lymphocytes Temperature therapeutics Veterinary medicine
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