Coding-Sequence Determinants of Gene Expression in Escherichia coli

Synonymous mutations do not alter the encoded protein, but they can influence gene expression. To investigate how, we engineered a synthetic library of 154 genes that varied randomly at synonymous sites, but all encoded the same green fluorescent protein (GFP). When expressed in Escherichia coli, GF...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2009-04, Vol.324 (5924), p.255-258
Hauptverfasser:	Kudla, Grzegorz, Murray, Andrew W, Tollervey, David, Plotkin, Joshua B
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Substitution Bacteriology Base Composition Biological and medical sciences Cloning, Molecular Codon Codons E coli Escherichia coli Escherichia coli - genetics Escherichia coli - growth & development Escherichia coli - metabolism Fluorescence Fundamental and applied biological sciences. Psychology Gene Expression Gene Library Genes, Synthetic Genetic mutation Genetic variation Genetics Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Libraries Messenger RNA Microbiology Mutation Nucleic Acid Conformation Nucleotides Protein Biosynthesis Proteins Ribonucleic acid RNA RNA Stability RNA, Bacterial - chemistry RNA, Bacterial - genetics RNA, Bacterial - metabolism RNA, Messenger - chemistry RNA, Messenger - genetics RNA, Messenger - metabolism Spectrometry, Fluorescence Start codon
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