Dissolvable Microneedle Arrays for Intradermal Delivery of Biologics: Fabrication and Application
ABSTRACT Purpose Design and evaluate a new micro-machining based approach for fabricating dissolvable microneedle arrays (MNAs) with diverse geometries and from different materials for dry delivery to skin microenvironments. The aims are to describe the new fabrication method, to evaluate geometric...
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| Veröffentlicht in: | Pharmaceutical research 2014-01, Vol.31 (1), p.117-135 |
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| creator | Bediz, Bekir Korkmaz, Emrullah Khilwani, Rakesh Donahue, Cara Erdos, Geza Falo, Louis D. Ozdoganlar, O. Burak |
| description | ABSTRACT
Purpose
Design and evaluate a new micro-machining based approach for fabricating dissolvable microneedle arrays (MNAs) with diverse geometries and from different materials for dry delivery to skin microenvironments. The aims are to describe the new fabrication method, to evaluate geometric and material capability as well as reproducibility of the method, and to demonstrate the effectiveness of fabricated MNAs in delivering bioactive molecules.
Methods
Precise master molds were created using micromilling. Micromolding was used to create elastomer production molds from master molds. The dissolvable MNAs were then fabricated using the spin-casting method. Fabricated MNAs with different geometries were evaluated for reproducibility. MNAs from different materials were fabricated to show material capability. MNAs with embedded bioactive components were tested for functionality on human and mice skin.
Results
MNAs with different geometries and from carboxymethyl cellulose, polyvinyl pyrrolidone and maltodextrin were created reproducibly using our method. MNAs successfully pierce the skin, precisely deliver their bioactive cargo to skin and induce specific immunity in mice.
Conclusions
We demonstrated that the new fabrication approach enables creating dissolvable MNAs with diverse geometries and from different materials reproducibly. We also demonstrated the application of MNAs for precise and specific delivery of biomolecules to skin microenvironments
in vitro
and
in vivo
. |
| doi_str_mv | 10.1007/s11095-013-1137-x |
| format | Article |
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Purpose
Design and evaluate a new micro-machining based approach for fabricating dissolvable microneedle arrays (MNAs) with diverse geometries and from different materials for dry delivery to skin microenvironments. The aims are to describe the new fabrication method, to evaluate geometric and material capability as well as reproducibility of the method, and to demonstrate the effectiveness of fabricated MNAs in delivering bioactive molecules.
Methods
Precise master molds were created using micromilling. Micromolding was used to create elastomer production molds from master molds. The dissolvable MNAs were then fabricated using the spin-casting method. Fabricated MNAs with different geometries were evaluated for reproducibility. MNAs from different materials were fabricated to show material capability. MNAs with embedded bioactive components were tested for functionality on human and mice skin.
Results
MNAs with different geometries and from carboxymethyl cellulose, polyvinyl pyrrolidone and maltodextrin were created reproducibly using our method. MNAs successfully pierce the skin, precisely deliver their bioactive cargo to skin and induce specific immunity in mice.
Conclusions
We demonstrated that the new fabrication approach enables creating dissolvable MNAs with diverse geometries and from different materials reproducibly. We also demonstrated the application of MNAs for precise and specific delivery of biomolecules to skin microenvironments
in vitro
and
in vivo
.</description><identifier>ISSN: 0724-8741</identifier><identifier>EISSN: 1573-904X</identifier><identifier>DOI: 10.1007/s11095-013-1137-x</identifier><identifier>PMID: 23904139</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Administration, Cutaneous ; Animals ; Biochemistry ; Biological products ; Biological Products - administration & dosage ; Biomedical and Life Sciences ; Biomedical Engineering and Bioengineering ; Biomedicine ; Drug delivery systems ; Drug Delivery Systems - instrumentation ; Drug Delivery Systems - methods ; Equipment Design - instrumentation ; Humans ; Medical Law ; Mice ; Microinjections - instrumentation ; Microinjections - methods ; Needles ; Pharmacology ; Pharmacology/Toxicology ; Pharmacy ; Reproducibility of Results ; Research Paper ; Skin ; Skin - metabolism</subject><ispartof>Pharmaceutical research, 2014-01, Vol.31 (1), p.117-135</ispartof><rights>Springer Science+Business Media New York 2013</rights><rights>Springer Science+Business Media New York 2014</rights><rights>Springer Science+Business Media New York 2013 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-cbabd63755ada501c52c3e7166f89bc3eadef43ea16b8e6e96a4b4ff5fcf66923</citedby><cites>FETCH-LOGICAL-c536t-cbabd63755ada501c52c3e7166f89bc3eadef43ea16b8e6e96a4b4ff5fcf66923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11095-013-1137-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11095-013-1137-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23904139$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bediz, Bekir</creatorcontrib><creatorcontrib>Korkmaz, Emrullah</creatorcontrib><creatorcontrib>Khilwani, Rakesh</creatorcontrib><creatorcontrib>Donahue, Cara</creatorcontrib><creatorcontrib>Erdos, Geza</creatorcontrib><creatorcontrib>Falo, Louis D.</creatorcontrib><creatorcontrib>Ozdoganlar, O. Burak</creatorcontrib><title>Dissolvable Microneedle Arrays for Intradermal Delivery of Biologics: Fabrication and Application</title><title>Pharmaceutical research</title><addtitle>Pharm Res</addtitle><addtitle>Pharm Res</addtitle><description>ABSTRACT
Purpose
Design and evaluate a new micro-machining based approach for fabricating dissolvable microneedle arrays (MNAs) with diverse geometries and from different materials for dry delivery to skin microenvironments. The aims are to describe the new fabrication method, to evaluate geometric and material capability as well as reproducibility of the method, and to demonstrate the effectiveness of fabricated MNAs in delivering bioactive molecules.
Methods
Precise master molds were created using micromilling. Micromolding was used to create elastomer production molds from master molds. The dissolvable MNAs were then fabricated using the spin-casting method. Fabricated MNAs with different geometries were evaluated for reproducibility. MNAs from different materials were fabricated to show material capability. MNAs with embedded bioactive components were tested for functionality on human and mice skin.
Results
MNAs with different geometries and from carboxymethyl cellulose, polyvinyl pyrrolidone and maltodextrin were created reproducibly using our method. MNAs successfully pierce the skin, precisely deliver their bioactive cargo to skin and induce specific immunity in mice.
Conclusions
We demonstrated that the new fabrication approach enables creating dissolvable MNAs with diverse geometries and from different materials reproducibly. We also demonstrated the application of MNAs for precise and specific delivery of biomolecules to skin microenvironments
in vitro
and
in vivo
.</description><subject>Administration, Cutaneous</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biological products</subject><subject>Biological Products - administration & dosage</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedicine</subject><subject>Drug delivery systems</subject><subject>Drug Delivery Systems - instrumentation</subject><subject>Drug Delivery Systems - methods</subject><subject>Equipment Design - instrumentation</subject><subject>Humans</subject><subject>Medical Law</subject><subject>Mice</subject><subject>Microinjections - instrumentation</subject><subject>Microinjections - methods</subject><subject>Needles</subject><subject>Pharmacology</subject><subject>Pharmacology/Toxicology</subject><subject>Pharmacy</subject><subject>Reproducibility of Results</subject><subject>Research Paper</subject><subject>Skin</subject><subject>Skin - metabolism</subject><issn>0724-8741</issn><issn>1573-904X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kU1rGzEQhkVpadykP6CXIuill001q69VDwUnaT4gpZcEchNareQqyCtXsk387ytjNySBnGbEPPNqZl6EPgE5BkLktwJAFG8I0AaAyubhDZoAl7RRhN29RRMiW9Z0ksEB-lDKPSGkA8Xeo4OWVgKomiBzFkpJcW366PCvYHManRtqPs3ZbAr2KeOrcZnN4PLcRHzmYli7vMHJ45OQYpoFW77jc9PnYM0ypBGbccDTxSLu30fonTexuI_7eIhuz3_enF42178vrk6n143lVCwb25t-EFRybgbDCVjeWuokCOE71de0TuBZDSD6zgmnhGE9855764VQLT1EP3a6i1U_d4N126mjXuQwN3mjkwn6eWUMf_QsrTXtVMcErwJf9wI5_V25stTzUKyL0YwurYoGpohk9WxQ0S8v0Pu0ymNdr1JCScIUZZWCHVWvWkp2_nEYIHproN4ZqKuBemugfqg9n59u8djx37EKtDug1NI4c_nJ16-q_gMI2al4</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Bediz, Bekir</creator><creator>Korkmaz, Emrullah</creator><creator>Khilwani, Rakesh</creator><creator>Donahue, Cara</creator><creator>Erdos, Geza</creator><creator>Falo, Louis D.</creator><creator>Ozdoganlar, O. Burak</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140101</creationdate><title>Dissolvable Microneedle Arrays for Intradermal Delivery of Biologics: Fabrication and Application</title><author>Bediz, Bekir ; Korkmaz, Emrullah ; Khilwani, Rakesh ; Donahue, Cara ; Erdos, Geza ; Falo, Louis D. ; Ozdoganlar, O. Burak</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c536t-cbabd63755ada501c52c3e7166f89bc3eadef43ea16b8e6e96a4b4ff5fcf66923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Administration, Cutaneous</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biological products</topic><topic>Biological Products - administration & dosage</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomedicine</topic><topic>Drug delivery systems</topic><topic>Drug Delivery Systems - instrumentation</topic><topic>Drug Delivery Systems - methods</topic><topic>Equipment Design - instrumentation</topic><topic>Humans</topic><topic>Medical Law</topic><topic>Mice</topic><topic>Microinjections - instrumentation</topic><topic>Microinjections - methods</topic><topic>Needles</topic><topic>Pharmacology</topic><topic>Pharmacology/Toxicology</topic><topic>Pharmacy</topic><topic>Reproducibility of Results</topic><topic>Research Paper</topic><topic>Skin</topic><topic>Skin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bediz, Bekir</creatorcontrib><creatorcontrib>Korkmaz, Emrullah</creatorcontrib><creatorcontrib>Khilwani, Rakesh</creatorcontrib><creatorcontrib>Donahue, Cara</creatorcontrib><creatorcontrib>Erdos, Geza</creatorcontrib><creatorcontrib>Falo, Louis D.</creatorcontrib><creatorcontrib>Ozdoganlar, O. Burak</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Pharmaceutical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bediz, Bekir</au><au>Korkmaz, Emrullah</au><au>Khilwani, Rakesh</au><au>Donahue, Cara</au><au>Erdos, Geza</au><au>Falo, Louis D.</au><au>Ozdoganlar, O. Burak</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dissolvable Microneedle Arrays for Intradermal Delivery of Biologics: Fabrication and Application</atitle><jtitle>Pharmaceutical research</jtitle><stitle>Pharm Res</stitle><addtitle>Pharm Res</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>31</volume><issue>1</issue><spage>117</spage><epage>135</epage><pages>117-135</pages><issn>0724-8741</issn><eissn>1573-904X</eissn><abstract>ABSTRACT
Purpose
Design and evaluate a new micro-machining based approach for fabricating dissolvable microneedle arrays (MNAs) with diverse geometries and from different materials for dry delivery to skin microenvironments. The aims are to describe the new fabrication method, to evaluate geometric and material capability as well as reproducibility of the method, and to demonstrate the effectiveness of fabricated MNAs in delivering bioactive molecules.
Methods
Precise master molds were created using micromilling. Micromolding was used to create elastomer production molds from master molds. The dissolvable MNAs were then fabricated using the spin-casting method. Fabricated MNAs with different geometries were evaluated for reproducibility. MNAs from different materials were fabricated to show material capability. MNAs with embedded bioactive components were tested for functionality on human and mice skin.
Results
MNAs with different geometries and from carboxymethyl cellulose, polyvinyl pyrrolidone and maltodextrin were created reproducibly using our method. MNAs successfully pierce the skin, precisely deliver their bioactive cargo to skin and induce specific immunity in mice.
Conclusions
We demonstrated that the new fabrication approach enables creating dissolvable MNAs with diverse geometries and from different materials reproducibly. We also demonstrated the application of MNAs for precise and specific delivery of biomolecules to skin microenvironments
in vitro
and
in vivo
.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>23904139</pmid><doi>10.1007/s11095-013-1137-x</doi><tpages>19</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Pharmaceutical research, 2014-01, Vol.31 (1), p.117-135 |
| issn | 0724-8741 1573-904X |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Administration, Cutaneous Animals Biochemistry Biological products Biological Products - administration & dosage Biomedical and Life Sciences Biomedical Engineering and Bioengineering Biomedicine Drug delivery systems Drug Delivery Systems - instrumentation Drug Delivery Systems - methods Equipment Design - instrumentation Humans Medical Law Mice Microinjections - instrumentation Microinjections - methods Needles Pharmacology Pharmacology/Toxicology Pharmacy Reproducibility of Results Research Paper Skin Skin - metabolism |
| title | Dissolvable Microneedle Arrays for Intradermal Delivery of Biologics: Fabrication and Application |
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