Micro-a-fluidics ELISA for Rapid CD4 Cell Count at the Point-of-Care
HIV has become one of the most devastating pathogens in human history. Despite fast progress in HIV-related basic research, antiretroviral therapy (ART) remains the most effective method to save AIDS patients' lives. Unfortunately, ART cannot be universally accessed, especially in developing co...
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| Veröffentlicht in: | Scientific reports 2014-01, Vol.4 (1), p.3796-3796, Article 3796 |
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| creator | Wang, ShuQi Tasoglu, Savas Chen, Paul Z. Chen, Michael Akbas, Ragip Wach, Sonya Ozdemir, Cenk Ibrahim Gurkan, Umut Atakan Giguel, Francoise F. Kuritzkes, Daniel R. Demirci, Utkan |
| description | HIV has become one of the most devastating pathogens in human history. Despite fast progress in HIV-related basic research, antiretroviral therapy (ART) remains the most effective method to save AIDS patients' lives. Unfortunately, ART cannot be universally accessed, especially in developing countries, due to the lack of effective treatment monitoring diagnostics. Here, we present an inexpensive, rapid and portable micro-a-fluidic platform, which can streamline the process of an enzyme-linked immunosorbent assay (ELISA) in a fully automated manner for CD4 cell count. The micro-a-fluidic CD4 cell count is achieved by eliminating operational fluid flow via “moving the substrate”, as opposed to “flowing liquid” in traditional ELISA or microfluidic methods. This is the first demonstration of capturing and detecting cells from unprocessed whole blood using the enzyme-linked immunosorbent assay (ELISA) in a microfluidic channel. Combined with cell phone imaging, the presented micro-a-fluidic ELISA platform holds great promise for offering rapid CD4 cell count to scale up much needed ART in resource-constrained settings. The developed system can be extended to multiple areas for ELISA-related assays. |
| doi_str_mv | 10.1038/srep03796 |
| format | Article |
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Despite fast progress in HIV-related basic research, antiretroviral therapy (ART) remains the most effective method to save AIDS patients' lives. Unfortunately, ART cannot be universally accessed, especially in developing countries, due to the lack of effective treatment monitoring diagnostics. Here, we present an inexpensive, rapid and portable micro-a-fluidic platform, which can streamline the process of an enzyme-linked immunosorbent assay (ELISA) in a fully automated manner for CD4 cell count. The micro-a-fluidic CD4 cell count is achieved by eliminating operational fluid flow via “moving the substrate”, as opposed to “flowing liquid” in traditional ELISA or microfluidic methods. This is the first demonstration of capturing and detecting cells from unprocessed whole blood using the enzyme-linked immunosorbent assay (ELISA) in a microfluidic channel. Combined with cell phone imaging, the presented micro-a-fluidic ELISA platform holds great promise for offering rapid CD4 cell count to scale up much needed ART in resource-constrained settings. The developed system can be extended to multiple areas for ELISA-related assays.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep03796</identifier><identifier>PMID: 24448112</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/62 ; 14/56 ; 692/308/575 ; 692/700/139/1420 ; 9/10 ; Acquired immune deficiency syndrome ; AIDS ; Antiretroviral agents ; Antiretroviral therapy ; CD4 antigen ; CD4 Lymphocyte Count ; CD4-Positive T-Lymphocytes - immunology ; CD4-Positive T-Lymphocytes - virology ; Cell Phone ; Developing countries ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Enzymes ; Flow Cytometry ; Fluid flow ; HIV Infections - blood ; HIV Infections - diagnosis ; HIV Infections - immunology ; HIV Infections - virology ; HIV-1 - pathogenicity ; Humanities and Social Sciences ; Humans ; LDCs ; Microfluidics ; Microfluidics - instrumentation ; Microfluidics - methods ; multidisciplinary ; Point-of-Care Systems ; Science ; Viral Load</subject><ispartof>Scientific reports, 2014-01, Vol.4 (1), p.3796-3796, Article 3796</ispartof><rights>The Author(s) 2014</rights><rights>Copyright Nature Publishing Group Jan 2014</rights><rights>Copyright © 2014, Macmillan Publishers Limited. All rights reserved 2014 Macmillan Publishers Limited. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-622aa4e8eeddf141658093caf32c2f572074af12ceb4cbbe288f45e0051c45c73</citedby><cites>FETCH-LOGICAL-c438t-622aa4e8eeddf141658093caf32c2f572074af12ceb4cbbe288f45e0051c45c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898414/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898414/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24448112$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, ShuQi</creatorcontrib><creatorcontrib>Tasoglu, Savas</creatorcontrib><creatorcontrib>Chen, Paul Z.</creatorcontrib><creatorcontrib>Chen, Michael</creatorcontrib><creatorcontrib>Akbas, Ragip</creatorcontrib><creatorcontrib>Wach, Sonya</creatorcontrib><creatorcontrib>Ozdemir, Cenk Ibrahim</creatorcontrib><creatorcontrib>Gurkan, Umut Atakan</creatorcontrib><creatorcontrib>Giguel, Francoise F.</creatorcontrib><creatorcontrib>Kuritzkes, Daniel R.</creatorcontrib><creatorcontrib>Demirci, Utkan</creatorcontrib><title>Micro-a-fluidics ELISA for Rapid CD4 Cell Count at the Point-of-Care</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>HIV has become one of the most devastating pathogens in human history. Despite fast progress in HIV-related basic research, antiretroviral therapy (ART) remains the most effective method to save AIDS patients' lives. Unfortunately, ART cannot be universally accessed, especially in developing countries, due to the lack of effective treatment monitoring diagnostics. Here, we present an inexpensive, rapid and portable micro-a-fluidic platform, which can streamline the process of an enzyme-linked immunosorbent assay (ELISA) in a fully automated manner for CD4 cell count. The micro-a-fluidic CD4 cell count is achieved by eliminating operational fluid flow via “moving the substrate”, as opposed to “flowing liquid” in traditional ELISA or microfluidic methods. This is the first demonstration of capturing and detecting cells from unprocessed whole blood using the enzyme-linked immunosorbent assay (ELISA) in a microfluidic channel. Combined with cell phone imaging, the presented micro-a-fluidic ELISA platform holds great promise for offering rapid CD4 cell count to scale up much needed ART in resource-constrained settings. The developed system can be extended to multiple areas for ELISA-related assays.</description><subject>13/62</subject><subject>14/56</subject><subject>692/308/575</subject><subject>692/700/139/1420</subject><subject>9/10</subject><subject>Acquired immune deficiency syndrome</subject><subject>AIDS</subject><subject>Antiretroviral agents</subject><subject>Antiretroviral therapy</subject><subject>CD4 antigen</subject><subject>CD4 Lymphocyte Count</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>CD4-Positive T-Lymphocytes - virology</subject><subject>Cell Phone</subject><subject>Developing countries</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzymes</subject><subject>Flow Cytometry</subject><subject>Fluid flow</subject><subject>HIV Infections - blood</subject><subject>HIV Infections - diagnosis</subject><subject>HIV Infections - immunology</subject><subject>HIV Infections - virology</subject><subject>HIV-1 - pathogenicity</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>LDCs</subject><subject>Microfluidics</subject><subject>Microfluidics - instrumentation</subject><subject>Microfluidics - methods</subject><subject>multidisciplinary</subject><subject>Point-of-Care Systems</subject><subject>Science</subject><subject>Viral Load</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkVtLxDAQhYMoKuqDf0ACvqhQzWXapi_CUq-wonh5Dtk00Ui3WZNW8N8bWV1WzcsE5uPMmTkI7VJyTAkXJzGYGeFlVaygTUYgzxhnbHXpv4F2Ynwl6eWsAlqtow0GAIJStonObpwOPlOZbQfXOB3x-fj6YYStD_hezVyD6zPAtWlbXPuh67Hqcf9i8J13XZ95m9UqmG20ZlUbzc533UJPF-eP9VU2vr28rkfjTAMXfVYwphQYYUzTWAq0yAWpuFaWM81sXjJSgrKUaTMBPZkYJoSF3CTfVEOuS76FTue6s2EyNY02XR9UK2fBTVX4kF45-bvTuRf57N8lF5UACkng4Fsg-LfBxF5OXdRpOdUZP0RJoWJFWQjyhe7_QV_9ELq0nqSiKgVhUPBEHc6pdMSYkrALM5TIr3jkIp7E7i27X5A_YSTgaA7E1OqeTVga-U_tEwGcluA</recordid><startdate>20140122</startdate><enddate>20140122</enddate><creator>Wang, ShuQi</creator><creator>Tasoglu, Savas</creator><creator>Chen, Paul Z.</creator><creator>Chen, Michael</creator><creator>Akbas, Ragip</creator><creator>Wach, Sonya</creator><creator>Ozdemir, Cenk Ibrahim</creator><creator>Gurkan, Umut Atakan</creator><creator>Giguel, Francoise F.</creator><creator>Kuritzkes, Daniel R.</creator><creator>Demirci, Utkan</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140122</creationdate><title>Micro-a-fluidics ELISA for Rapid CD4 Cell Count at the Point-of-Care</title><author>Wang, ShuQi ; Tasoglu, Savas ; Chen, Paul Z. ; Chen, Michael ; Akbas, Ragip ; Wach, Sonya ; Ozdemir, Cenk Ibrahim ; Gurkan, Umut Atakan ; Giguel, Francoise F. ; Kuritzkes, Daniel R. ; Demirci, Utkan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-622aa4e8eeddf141658093caf32c2f572074af12ceb4cbbe288f45e0051c45c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>13/62</topic><topic>14/56</topic><topic>692/308/575</topic><topic>692/700/139/1420</topic><topic>9/10</topic><topic>Acquired immune deficiency syndrome</topic><topic>AIDS</topic><topic>Antiretroviral agents</topic><topic>Antiretroviral therapy</topic><topic>CD4 antigen</topic><topic>CD4 Lymphocyte Count</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>CD4-Positive T-Lymphocytes - virology</topic><topic>Cell Phone</topic><topic>Developing countries</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzymes</topic><topic>Flow Cytometry</topic><topic>Fluid flow</topic><topic>HIV Infections - blood</topic><topic>HIV Infections - diagnosis</topic><topic>HIV Infections - immunology</topic><topic>HIV Infections - virology</topic><topic>HIV-1 - pathogenicity</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>LDCs</topic><topic>Microfluidics</topic><topic>Microfluidics - instrumentation</topic><topic>Microfluidics - methods</topic><topic>multidisciplinary</topic><topic>Point-of-Care Systems</topic><topic>Science</topic><topic>Viral Load</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, ShuQi</creatorcontrib><creatorcontrib>Tasoglu, Savas</creatorcontrib><creatorcontrib>Chen, Paul Z.</creatorcontrib><creatorcontrib>Chen, Michael</creatorcontrib><creatorcontrib>Akbas, Ragip</creatorcontrib><creatorcontrib>Wach, Sonya</creatorcontrib><creatorcontrib>Ozdemir, Cenk Ibrahim</creatorcontrib><creatorcontrib>Gurkan, Umut Atakan</creatorcontrib><creatorcontrib>Giguel, Francoise F.</creatorcontrib><creatorcontrib>Kuritzkes, Daniel R.</creatorcontrib><creatorcontrib>Demirci, Utkan</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database (ProQuest)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, ShuQi</au><au>Tasoglu, Savas</au><au>Chen, Paul Z.</au><au>Chen, Michael</au><au>Akbas, Ragip</au><au>Wach, Sonya</au><au>Ozdemir, Cenk Ibrahim</au><au>Gurkan, Umut Atakan</au><au>Giguel, Francoise F.</au><au>Kuritzkes, Daniel R.</au><au>Demirci, Utkan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Micro-a-fluidics ELISA for Rapid CD4 Cell Count at the Point-of-Care</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2014-01-22</date><risdate>2014</risdate><volume>4</volume><issue>1</issue><spage>3796</spage><epage>3796</epage><pages>3796-3796</pages><artnum>3796</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>HIV has become one of the most devastating pathogens in human history. Despite fast progress in HIV-related basic research, antiretroviral therapy (ART) remains the most effective method to save AIDS patients' lives. Unfortunately, ART cannot be universally accessed, especially in developing countries, due to the lack of effective treatment monitoring diagnostics. Here, we present an inexpensive, rapid and portable micro-a-fluidic platform, which can streamline the process of an enzyme-linked immunosorbent assay (ELISA) in a fully automated manner for CD4 cell count. The micro-a-fluidic CD4 cell count is achieved by eliminating operational fluid flow via “moving the substrate”, as opposed to “flowing liquid” in traditional ELISA or microfluidic methods. This is the first demonstration of capturing and detecting cells from unprocessed whole blood using the enzyme-linked immunosorbent assay (ELISA) in a microfluidic channel. Combined with cell phone imaging, the presented micro-a-fluidic ELISA platform holds great promise for offering rapid CD4 cell count to scale up much needed ART in resource-constrained settings. The developed system can be extended to multiple areas for ELISA-related assays.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>24448112</pmid><doi>10.1038/srep03796</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 13/62 14/56 692/308/575 692/700/139/1420 9/10 Acquired immune deficiency syndrome AIDS Antiretroviral agents Antiretroviral therapy CD4 antigen CD4 Lymphocyte Count CD4-Positive T-Lymphocytes - immunology CD4-Positive T-Lymphocytes - virology Cell Phone Developing countries Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Enzymes Flow Cytometry Fluid flow HIV Infections - blood HIV Infections - diagnosis HIV Infections - immunology HIV Infections - virology HIV-1 - pathogenicity Humanities and Social Sciences Humans LDCs Microfluidics Microfluidics - instrumentation Microfluidics - methods multidisciplinary Point-of-Care Systems Science Viral Load |
| title | Micro-a-fluidics ELISA for Rapid CD4 Cell Count at the Point-of-Care |
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