Non-invasive airway health assessment: Synchrotron imaging reveals effects of rehydrating treatments on mucociliary transit in-vivo
To determine the efficacy of potential cystic fibrosis (CF) therapies we have developed a novel mucociliary transit (MCT) measurement that uses synchrotron phase contrast X-ray imaging (PCXI) to non-invasively measure the transit rate of individual micron-sized particles deposited into the airways o...
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| Veröffentlicht in: | Scientific reports 2014-01, Vol.4 (1), p.3689-3689, Article 3689 |
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| creator | Donnelley, Martin Morgan, Kaye S. Siu, Karen K. W. Farrow, Nigel R. Stahr, Charlene S. Boucher, Richard C. Fouras, Andreas Parsons, David W. |
| description | To determine the efficacy of potential cystic fibrosis (CF) therapies we have developed a novel mucociliary transit (MCT) measurement that uses synchrotron phase contrast X-ray imaging (PCXI) to non-invasively measure the transit rate of individual micron-sized particles deposited into the airways of live mice. The aim of this study was to image changes in MCT produced by a rehydrating treatment based on hypertonic saline (HS), a current CF clinical treatment. Live mice received HS containing a long acting epithelial sodium channel blocker (P308); isotonic saline; or no treatment, using a nebuliser integrated within a small-animal ventilator circuit. Marker particle motion was tracked for 20 minutes using PCXI. There were statistically significant increases in MCT in the isotonic and HS-P308 groups. The ability to quantify
in vivo
changes in MCT may have utility in pre-clinical research studies designed to bring new genetic and pharmaceutical treatments for respiratory diseases into clinical trials. |
| doi_str_mv | 10.1038/srep03689 |
| format | Article |
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in vivo
changes in MCT may have utility in pre-clinical research studies designed to bring new genetic and pharmaceutical treatments for respiratory diseases into clinical trials.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep03689</identifier><identifier>PMID: 24418935</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>14/63 ; 59 ; 639/166/985 ; 64/60 ; 692/308/2778 ; 692/698 ; Animals ; Clinical trials ; Cystic fibrosis ; Cystic Fibrosis - diagnosis ; Cystic Fibrosis - diagnostic imaging ; Diagnostic Imaging - methods ; Humanities and Social Sciences ; Mice ; Mice, Inbred C57BL ; multidisciplinary ; Particle Size ; Radionuclide Imaging ; Respiratory diseases ; Respiratory System - diagnostic imaging ; Respiratory tract ; Rodents ; Saline Solution, Hypertonic - administration & dosage ; Science ; Sodium ; Statistical analysis ; Synchrotrons ; X-Rays</subject><ispartof>Scientific reports, 2014-01, Vol.4 (1), p.3689-3689, Article 3689</ispartof><rights>The Author(s) 2014</rights><rights>Copyright Nature Publishing Group Jan 2014</rights><rights>Copyright © 2014, Macmillan Publishers Limited. All rights reserved 2014 Macmillan Publishers Limited. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-d5fa4153691cc15496270ba3d679beea18d2f7d7be2c779949e037c9afe843433</citedby><cites>FETCH-LOGICAL-c438t-d5fa4153691cc15496270ba3d679beea18d2f7d7be2c779949e037c9afe843433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3891397/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3891397/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,41099,42168,51554,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24418935$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Donnelley, Martin</creatorcontrib><creatorcontrib>Morgan, Kaye S.</creatorcontrib><creatorcontrib>Siu, Karen K. W.</creatorcontrib><creatorcontrib>Farrow, Nigel R.</creatorcontrib><creatorcontrib>Stahr, Charlene S.</creatorcontrib><creatorcontrib>Boucher, Richard C.</creatorcontrib><creatorcontrib>Fouras, Andreas</creatorcontrib><creatorcontrib>Parsons, David W.</creatorcontrib><title>Non-invasive airway health assessment: Synchrotron imaging reveals effects of rehydrating treatments on mucociliary transit in-vivo</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>To determine the efficacy of potential cystic fibrosis (CF) therapies we have developed a novel mucociliary transit (MCT) measurement that uses synchrotron phase contrast X-ray imaging (PCXI) to non-invasively measure the transit rate of individual micron-sized particles deposited into the airways of live mice. The aim of this study was to image changes in MCT produced by a rehydrating treatment based on hypertonic saline (HS), a current CF clinical treatment. Live mice received HS containing a long acting epithelial sodium channel blocker (P308); isotonic saline; or no treatment, using a nebuliser integrated within a small-animal ventilator circuit. Marker particle motion was tracked for 20 minutes using PCXI. There were statistically significant increases in MCT in the isotonic and HS-P308 groups. The ability to quantify
in vivo
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W.</creatorcontrib><creatorcontrib>Farrow, Nigel R.</creatorcontrib><creatorcontrib>Stahr, Charlene S.</creatorcontrib><creatorcontrib>Boucher, Richard C.</creatorcontrib><creatorcontrib>Fouras, Andreas</creatorcontrib><creatorcontrib>Parsons, David W.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Donnelley, Martin</au><au>Morgan, Kaye S.</au><au>Siu, Karen K. W.</au><au>Farrow, Nigel R.</au><au>Stahr, Charlene S.</au><au>Boucher, Richard C.</au><au>Fouras, Andreas</au><au>Parsons, David W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Non-invasive airway health assessment: Synchrotron imaging reveals effects of rehydrating treatments on mucociliary transit in-vivo</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2014-01-14</date><risdate>2014</risdate><volume>4</volume><issue>1</issue><spage>3689</spage><epage>3689</epage><pages>3689-3689</pages><artnum>3689</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>To determine the efficacy of potential cystic fibrosis (CF) therapies we have developed a novel mucociliary transit (MCT) measurement that uses synchrotron phase contrast X-ray imaging (PCXI) to non-invasively measure the transit rate of individual micron-sized particles deposited into the airways of live mice. The aim of this study was to image changes in MCT produced by a rehydrating treatment based on hypertonic saline (HS), a current CF clinical treatment. Live mice received HS containing a long acting epithelial sodium channel blocker (P308); isotonic saline; or no treatment, using a nebuliser integrated within a small-animal ventilator circuit. Marker particle motion was tracked for 20 minutes using PCXI. There were statistically significant increases in MCT in the isotonic and HS-P308 groups. The ability to quantify
in vivo
changes in MCT may have utility in pre-clinical research studies designed to bring new genetic and pharmaceutical treatments for respiratory diseases into clinical trials.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>24418935</pmid><doi>10.1038/srep03689</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 14/63 59 639/166/985 64/60 692/308/2778 692/698 Animals Clinical trials Cystic fibrosis Cystic Fibrosis - diagnosis Cystic Fibrosis - diagnostic imaging Diagnostic Imaging - methods Humanities and Social Sciences Mice Mice, Inbred C57BL multidisciplinary Particle Size Radionuclide Imaging Respiratory diseases Respiratory System - diagnostic imaging Respiratory tract Rodents Saline Solution, Hypertonic - administration & dosage Science Sodium Statistical analysis Synchrotrons X-Rays |
| title | Non-invasive airway health assessment: Synchrotron imaging reveals effects of rehydrating treatments on mucociliary transit in-vivo |
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