Targeted disruption of the mouse transforming growth factor-β1 gene results in multifocal inflammatory disease

Transforming growth factor-β1 (TGF-β1) is a multifunctional growth factor that has profound regulatory effects on many developmental and physiological processes. Disruption of the TGF-β1 gene by homologous recombination in murine embryonic stem cells enables mice to be generated that carry the disru...

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Veröffentlicht in:Nature (London) 1992-10, Vol.359 (6397), p.693-699
Hauptverfasser: Shull, Marcia M., Ormsby, Ilona, Kier, Ann B., Pawlowski, Sharon, Diebold, Ronald J., Yin, Moying, Allen, Ruth, Sidman, Charles, Proetzel, Gabriele, Calvin, Dawn, Annunziata, Nikki, Doetschman, Thomas
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container_end_page 699
container_issue 6397
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container_title Nature (London)
container_volume 359
creator Shull, Marcia M.
Ormsby, Ilona
Kier, Ann B.
Pawlowski, Sharon
Diebold, Ronald J.
Yin, Moying
Allen, Ruth
Sidman, Charles
Proetzel, Gabriele
Calvin, Dawn
Annunziata, Nikki
Doetschman, Thomas
description Transforming growth factor-β1 (TGF-β1) is a multifunctional growth factor that has profound regulatory effects on many developmental and physiological processes. Disruption of the TGF-β1 gene by homologous recombination in murine embryonic stem cells enables mice to be generated that carry the disrupted allele. Animals homozygous for the mutated TGF-β1 allele show no gross developmental abnormalities, but about 20 days after birth they succumb to a wasting syndrome accompanied by a multifocal, mixed inflammatory cell response and tissue necrosis, leading to organ failure and death. TGF-β1-deficient mice may be valuable models for human immune and inflammatory disorders, including autoimmune diseases, transplant rejection and graft versus host reactions.
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Disruption of the TGF-β1 gene by homologous recombination in murine embryonic stem cells enables mice to be generated that carry the disrupted allele. Animals homozygous for the mutated TGF-β1 allele show no gross developmental abnormalities, but about 20 days after birth they succumb to a wasting syndrome accompanied by a multifocal, mixed inflammatory cell response and tissue necrosis, leading to organ failure and death. TGF-β1-deficient mice may be valuable models for human immune and inflammatory disorders, including autoimmune diseases, transplant rejection and graft versus host reactions.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/359693a0</identifier><identifier>PMID: 1436033</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Biochemistry ; Biological and medical sciences ; Experimental and animal immunopathology. 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source Nature Journals Online; SpringerLink Journals - AutoHoldings
subjects Biochemistry
Biological and medical sciences
Experimental and animal immunopathology. Animal models
Humanities and Social Sciences
Immunity (Disease)
Immunopathology
Medical research
Medical sciences
multidisciplinary
Rodents
Science
Science (multidisciplinary)
Stem cells
title Targeted disruption of the mouse transforming growth factor-β1 gene results in multifocal inflammatory disease
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