Active DNA demethylation is required for complete imprint erasure in primordial germ cells
In mammalian primordial germ cells (PGCs), DNA demethylation is indispensible for parental imprint erasure, which is a reprogramming process essential for normal developmental potential. Thus, it is important to elucidate how DNA demethylation occurs in each imprinted region in PGCs and to determine...
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| Veröffentlicht in: | Scientific reports 2014-01, Vol.4 (1), p.3658, Article 3658 |
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| creator | Kawasaki, Yuki Lee, Jiyoung Matsuzawa, Ayumi Kohda, Takashi Kaneko-Ishino, Tomoko Ishino, Fumitoshi |
| description | In mammalian primordial germ cells (PGCs), DNA demethylation is indispensible for parental imprint erasure, which is a reprogramming process essential for normal developmental potential. Thus, it is important to elucidate how DNA demethylation occurs in each imprinted region in PGCs and to determine which DNA demethylation pathway, passive or active, essentially contributes to the erasure of the imprint. Here, we report that active DNA demethylation via a putative Poly(ADP-ribose) polymerase (PARP) pathway is involved in
H19
-DMR imprint erasure in PGCs, as shown by an
in vivo
small molecule inhibitor assay. To the best of our knowledge, this is the first direct demonstration of a DNA replication-independent active DNA demethylation pathway in the erasure process of genomic imprinting in PGCs
in vivo
. The data also suggest that active DNA demethylation plays a significant role in the complete erasure of paternal imprinting in the female germ line. |
| doi_str_mv | 10.1038/srep03658 |
| format | Article |
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H19
-DMR imprint erasure in PGCs, as shown by an
in vivo
small molecule inhibitor assay. To the best of our knowledge, this is the first direct demonstration of a DNA replication-independent active DNA demethylation pathway in the erasure process of genomic imprinting in PGCs
in vivo
. The data also suggest that active DNA demethylation plays a significant role in the complete erasure of paternal imprinting in the female germ line.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep03658</identifier><identifier>PMID: 24413819</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13 ; 13/31 ; 45/22 ; 631/136/2434 ; 631/136/2435 ; 631/61/212/177 ; 631/80/641/151 ; Adenosine diphosphate ; Animals ; Cruelty to animals ; Demethylation ; Deoxyribonucleic acid ; DNA ; DNA biosynthesis ; DNA Methylation - drug effects ; Embryo, Mammalian ; Female ; Gene Expression Regulation, Developmental ; Genomic Imprinting ; Germ cells ; Germ Cells - drug effects ; Germ Cells - metabolism ; Humanities and Social Sciences ; Male ; Mice ; multidisciplinary ; Poly(ADP-ribose) ; Poly(ADP-ribose) polymerase ; Poly(ADP-ribose) Polymerase Inhibitors ; Poly(ADP-ribose) Polymerases - metabolism ; Ribose ; RNA, Long Noncoding - genetics ; Science</subject><ispartof>Scientific reports, 2014-01, Vol.4 (1), p.3658, Article 3658</ispartof><rights>The Author(s) 2014</rights><rights>Copyright Nature Publishing Group Jan 2014</rights><rights>Copyright © 2014, Macmillan Publishers Limited. All rights reserved 2014 Macmillan Publishers Limited. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-7ec097de7bff4fc666dea0323519ac7b56f5527ee12b5d273df8b28055336b313</citedby><cites>FETCH-LOGICAL-c504t-7ec097de7bff4fc666dea0323519ac7b56f5527ee12b5d273df8b28055336b313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3888974/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3888974/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,41099,42168,51554,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24413819$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kawasaki, Yuki</creatorcontrib><creatorcontrib>Lee, Jiyoung</creatorcontrib><creatorcontrib>Matsuzawa, Ayumi</creatorcontrib><creatorcontrib>Kohda, Takashi</creatorcontrib><creatorcontrib>Kaneko-Ishino, Tomoko</creatorcontrib><creatorcontrib>Ishino, Fumitoshi</creatorcontrib><title>Active DNA demethylation is required for complete imprint erasure in primordial germ cells</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>In mammalian primordial germ cells (PGCs), DNA demethylation is indispensible for parental imprint erasure, which is a reprogramming process essential for normal developmental potential. Thus, it is important to elucidate how DNA demethylation occurs in each imprinted region in PGCs and to determine which DNA demethylation pathway, passive or active, essentially contributes to the erasure of the imprint. Here, we report that active DNA demethylation via a putative Poly(ADP-ribose) polymerase (PARP) pathway is involved in
H19
-DMR imprint erasure in PGCs, as shown by an
in vivo
small molecule inhibitor assay. To the best of our knowledge, this is the first direct demonstration of a DNA replication-independent active DNA demethylation pathway in the erasure process of genomic imprinting in PGCs
in vivo
. The data also suggest that active DNA demethylation plays a significant role in the complete erasure of paternal imprinting in the female germ line.</description><subject>13</subject><subject>13/31</subject><subject>45/22</subject><subject>631/136/2434</subject><subject>631/136/2435</subject><subject>631/61/212/177</subject><subject>631/80/641/151</subject><subject>Adenosine diphosphate</subject><subject>Animals</subject><subject>Cruelty to animals</subject><subject>Demethylation</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA biosynthesis</subject><subject>DNA Methylation - drug effects</subject><subject>Embryo, Mammalian</subject><subject>Female</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genomic Imprinting</subject><subject>Germ cells</subject><subject>Germ Cells - drug effects</subject><subject>Germ Cells - metabolism</subject><subject>Humanities and Social Sciences</subject><subject>Male</subject><subject>Mice</subject><subject>multidisciplinary</subject><subject>Poly(ADP-ribose)</subject><subject>Poly(ADP-ribose) polymerase</subject><subject>Poly(ADP-ribose) Polymerase Inhibitors</subject><subject>Poly(ADP-ribose) Polymerases - metabolism</subject><subject>Ribose</subject><subject>RNA, Long Noncoding - genetics</subject><subject>Science</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkUtLAzEUhYMoVmoX_gEJuFKo5jGZx0Yo9QlFN7pxEzKZO23KzKRNZgr996a2lorZ3CT349zDuQhdUHJLCU_vvIMF4bFIj9AZI5EYMs7Y8cG9hwbez0k4gmURzU5Rj0UR5SnNztDXSLdmBfjhbYQLqKGdrSvVGttg47GDZWccFLi0DmtbLypoAZt64UzTYnDKdy68Gxw-ausKoyo8BVdjDVXlz9FJqSoPg13to8-nx4_xy3Dy_vw6Hk2GWpCoHSagSZYUkORlGZU6juMCFOGMC5opneQiLoVgCQBluShYwosyzVlKhOA8zjnlfXS_1V10eQ2FhqZ1qpIbT8qtpVVG_u00ZiandiV5mqZZEgWBq52As8sOfCvntnNN8CxpAJIsin-o6y2lnfUh9HI_gRK52YTcbyKwl4eW9uRv7gG42QJ-k2XI7GDkP7VvhDOT7Q</recordid><startdate>20140113</startdate><enddate>20140113</enddate><creator>Kawasaki, Yuki</creator><creator>Lee, Jiyoung</creator><creator>Matsuzawa, Ayumi</creator><creator>Kohda, Takashi</creator><creator>Kaneko-Ishino, Tomoko</creator><creator>Ishino, Fumitoshi</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20140113</creationdate><title>Active DNA demethylation is required for complete imprint erasure in primordial germ cells</title><author>Kawasaki, Yuki ; Lee, Jiyoung ; Matsuzawa, Ayumi ; Kohda, Takashi ; Kaneko-Ishino, Tomoko ; Ishino, Fumitoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-7ec097de7bff4fc666dea0323519ac7b56f5527ee12b5d273df8b28055336b313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>13</topic><topic>13/31</topic><topic>45/22</topic><topic>631/136/2434</topic><topic>631/136/2435</topic><topic>631/61/212/177</topic><topic>631/80/641/151</topic><topic>Adenosine diphosphate</topic><topic>Animals</topic><topic>Cruelty to animals</topic><topic>Demethylation</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA biosynthesis</topic><topic>DNA Methylation - drug effects</topic><topic>Embryo, Mammalian</topic><topic>Female</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genomic Imprinting</topic><topic>Germ cells</topic><topic>Germ Cells - drug effects</topic><topic>Germ Cells - metabolism</topic><topic>Humanities and Social Sciences</topic><topic>Male</topic><topic>Mice</topic><topic>multidisciplinary</topic><topic>Poly(ADP-ribose)</topic><topic>Poly(ADP-ribose) polymerase</topic><topic>Poly(ADP-ribose) Polymerase Inhibitors</topic><topic>Poly(ADP-ribose) Polymerases - metabolism</topic><topic>Ribose</topic><topic>RNA, Long Noncoding - genetics</topic><topic>Science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kawasaki, Yuki</creatorcontrib><creatorcontrib>Lee, Jiyoung</creatorcontrib><creatorcontrib>Matsuzawa, Ayumi</creatorcontrib><creatorcontrib>Kohda, Takashi</creatorcontrib><creatorcontrib>Kaneko-Ishino, Tomoko</creatorcontrib><creatorcontrib>Ishino, Fumitoshi</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawasaki, Yuki</au><au>Lee, Jiyoung</au><au>Matsuzawa, Ayumi</au><au>Kohda, Takashi</au><au>Kaneko-Ishino, Tomoko</au><au>Ishino, Fumitoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Active DNA demethylation is required for complete imprint erasure in primordial germ cells</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2014-01-13</date><risdate>2014</risdate><volume>4</volume><issue>1</issue><spage>3658</spage><pages>3658-</pages><artnum>3658</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>In mammalian primordial germ cells (PGCs), DNA demethylation is indispensible for parental imprint erasure, which is a reprogramming process essential for normal developmental potential. Thus, it is important to elucidate how DNA demethylation occurs in each imprinted region in PGCs and to determine which DNA demethylation pathway, passive or active, essentially contributes to the erasure of the imprint. Here, we report that active DNA demethylation via a putative Poly(ADP-ribose) polymerase (PARP) pathway is involved in
H19
-DMR imprint erasure in PGCs, as shown by an
in vivo
small molecule inhibitor assay. To the best of our knowledge, this is the first direct demonstration of a DNA replication-independent active DNA demethylation pathway in the erasure process of genomic imprinting in PGCs
in vivo
. The data also suggest that active DNA demethylation plays a significant role in the complete erasure of paternal imprinting in the female germ line.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>24413819</pmid><doi>10.1038/srep03658</doi><oa>free_for_read</oa></addata></record> |
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| subjects | 13 13/31 45/22 631/136/2434 631/136/2435 631/61/212/177 631/80/641/151 Adenosine diphosphate Animals Cruelty to animals Demethylation Deoxyribonucleic acid DNA DNA biosynthesis DNA Methylation - drug effects Embryo, Mammalian Female Gene Expression Regulation, Developmental Genomic Imprinting Germ cells Germ Cells - drug effects Germ Cells - metabolism Humanities and Social Sciences Male Mice multidisciplinary Poly(ADP-ribose) Poly(ADP-ribose) polymerase Poly(ADP-ribose) Polymerase Inhibitors Poly(ADP-ribose) Polymerases - metabolism Ribose RNA, Long Noncoding - genetics Science |
| title | Active DNA demethylation is required for complete imprint erasure in primordial germ cells |
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