Salvia macilenta exhibits antiglycating activity and protects PC12 cells against H2O2-induced apoptosis
Salvia macilenta is a member of the genus Salvia (Laminaceae) whose antioxidant activity and neuroprotective effect has been shown previously. The present study aimed to examine the antiglycating and antiapoptotic abilities of methanolic extract of this plant. Moreover, the effect of S. macilenta on...
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| Veröffentlicht in: | Cytotechnology (Dordrecht) 2014-01, Vol.66 (1), p.169-179 |
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| creator | Tusi, Solaleh Khoramian Khodagholi, Fariba |
| description | Salvia macilenta
is a member of the genus
Salvia
(Laminaceae) whose antioxidant activity and neuroprotective effect has been shown previously. The present study aimed to examine the antiglycating and antiapoptotic abilities of methanolic extract of this plant. Moreover, the effect of
S. macilenta
on neurite outgrowth and complexity after exposure to H
2
O
2
has been studied. Base on our results,
S. macilenta
has antiglycating activity and protects PC12 cells against oxidative stress-induced apoptotic cell death, as examined by Hoechst staining and Western blot analysis of caspase-3, Bax, Bcl-2 and PARP. We further showed that
S. macilenta
decreased neurite growth and complexity impairment in differentiated PC12 cells exposed to oxidative stress. It caused a decrease in cell body area, neurite width, and the proportion of bipolar cells, while significantly increasing neurite length, the number of primary neurites per cell and the ratio of nodes to primary neuritis. All around, the mentioned results open a new horizon for future works to use this plant as a potential neuroprotective agent. |
| doi_str_mv | 10.1007/s10616-013-9550-x |
| format | Article |
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is a member of the genus
Salvia
(Laminaceae) whose antioxidant activity and neuroprotective effect has been shown previously. The present study aimed to examine the antiglycating and antiapoptotic abilities of methanolic extract of this plant. Moreover, the effect of
S. macilenta
on neurite outgrowth and complexity after exposure to H
2
O
2
has been studied. Base on our results,
S. macilenta
has antiglycating activity and protects PC12 cells against oxidative stress-induced apoptotic cell death, as examined by Hoechst staining and Western blot analysis of caspase-3, Bax, Bcl-2 and PARP. We further showed that
S. macilenta
decreased neurite growth and complexity impairment in differentiated PC12 cells exposed to oxidative stress. It caused a decrease in cell body area, neurite width, and the proportion of bipolar cells, while significantly increasing neurite length, the number of primary neurites per cell and the ratio of nodes to primary neuritis. All around, the mentioned results open a new horizon for future works to use this plant as a potential neuroprotective agent.</description><identifier>ISSN: 0920-9069</identifier><identifier>EISSN: 1573-0778</identifier><identifier>DOI: 10.1007/s10616-013-9550-x</identifier><identifier>PMID: 23579246</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Antioxidants ; Apoptosis ; Axonogenesis ; BAX protein ; Bcl-2 protein ; Biochemistry ; Biomedicine ; Biotechnology ; Bipolar cells ; Caspase-3 ; Cell body ; Cell death ; Cell differentiation ; Chemistry ; Chemistry and Materials Science ; Enzymes ; Flavonoids ; Hydrogen peroxide ; Neuritis ; Neuroprotection ; Original Research ; Oxidation ; Oxidative stress ; Penicillin ; Pheochromocytoma cells ; Plant extracts ; Proteins</subject><ispartof>Cytotechnology (Dordrecht), 2014-01, Vol.66 (1), p.169-179</ispartof><rights>Springer Science+Business Media Dordrecht 2013</rights><rights>Springer Science+Business Media Dordrecht 2013.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-f57deabc25f0ab414ecf733284272d91c21514597fb2f097b3445073bb6faf333</citedby><cites>FETCH-LOGICAL-c414t-f57deabc25f0ab414ecf733284272d91c21514597fb2f097b3445073bb6faf333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3886546/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918253224?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,21388,27924,27925,33744,41488,42557,43805,51319,53791,53793,64385,64389,72469</link.rule.ids></links><search><creatorcontrib>Tusi, Solaleh Khoramian</creatorcontrib><creatorcontrib>Khodagholi, Fariba</creatorcontrib><title>Salvia macilenta exhibits antiglycating activity and protects PC12 cells against H2O2-induced apoptosis</title><title>Cytotechnology (Dordrecht)</title><addtitle>Cytotechnology</addtitle><description>Salvia macilenta
is a member of the genus
Salvia
(Laminaceae) whose antioxidant activity and neuroprotective effect has been shown previously. The present study aimed to examine the antiglycating and antiapoptotic abilities of methanolic extract of this plant. Moreover, the effect of
S. macilenta
on neurite outgrowth and complexity after exposure to H
2
O
2
has been studied. Base on our results,
S. macilenta
has antiglycating activity and protects PC12 cells against oxidative stress-induced apoptotic cell death, as examined by Hoechst staining and Western blot analysis of caspase-3, Bax, Bcl-2 and PARP. We further showed that
S. macilenta
decreased neurite growth and complexity impairment in differentiated PC12 cells exposed to oxidative stress. It caused a decrease in cell body area, neurite width, and the proportion of bipolar cells, while significantly increasing neurite length, the number of primary neurites per cell and the ratio of nodes to primary neuritis. All around, the mentioned results open a new horizon for future works to use this plant as a potential neuroprotective agent.</description><subject>Antioxidants</subject><subject>Apoptosis</subject><subject>Axonogenesis</subject><subject>BAX protein</subject><subject>Bcl-2 protein</subject><subject>Biochemistry</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Bipolar cells</subject><subject>Caspase-3</subject><subject>Cell body</subject><subject>Cell death</subject><subject>Cell differentiation</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Enzymes</subject><subject>Flavonoids</subject><subject>Hydrogen peroxide</subject><subject>Neuritis</subject><subject>Neuroprotection</subject><subject>Original Research</subject><subject>Oxidation</subject><subject>Oxidative stress</subject><subject>Penicillin</subject><subject>Pheochromocytoma cells</subject><subject>Plant extracts</subject><subject>Proteins</subject><issn>0920-9069</issn><issn>1573-0778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kUFrGzEQhUVoqJ2kPyC3hZ7VSiNptboUimnqQsCFJGeh1UobmfXuVpKN_e-j4NCSQ08DM997M8ND6JaSL5QQ-TVRUtMaE8qwEoLg4wVaUiEZJlI2H9CSKCBYkVot0FVKW0KIkpR9RAtgQirg9RL1D2Y4BFPtjA2DG7Op3PE5tCGnyow59MPJmhzGvjI2h0PIp9LuqjlO2dnC_F5RqKwbhoL3JowpV2vYAA5jt7euq8w8zXlKId2gS2-G5D691Wv0dPfjcbXG95ufv1bf77HllGfsheycaS0IT0xbWs56yRg0HCR0ilqggnKhpG_Bl29axrkgkrVt7Y1njF2jb2ffed_uXGfLS9EMeo5hZ-JJTybo95MxPOt-OmjWNLXgdTH4_GYQpz97l7LeTvs4lps1KNqAYAC8UPRM2TilFJ3_u4ES_ZqNPmejSzb6NRt9LBo4a1Jhx97Ff87_F70AqHaSLw</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Tusi, Solaleh Khoramian</creator><creator>Khodagholi, Fariba</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>5PM</scope></search><sort><creationdate>20140101</creationdate><title>Salvia macilenta exhibits antiglycating activity and protects PC12 cells against H2O2-induced apoptosis</title><author>Tusi, Solaleh Khoramian ; Khodagholi, Fariba</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-f57deabc25f0ab414ecf733284272d91c21514597fb2f097b3445073bb6faf333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Antioxidants</topic><topic>Apoptosis</topic><topic>Axonogenesis</topic><topic>BAX protein</topic><topic>Bcl-2 protein</topic><topic>Biochemistry</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Bipolar cells</topic><topic>Caspase-3</topic><topic>Cell body</topic><topic>Cell death</topic><topic>Cell differentiation</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Enzymes</topic><topic>Flavonoids</topic><topic>Hydrogen peroxide</topic><topic>Neuritis</topic><topic>Neuroprotection</topic><topic>Original Research</topic><topic>Oxidation</topic><topic>Oxidative stress</topic><topic>Penicillin</topic><topic>Pheochromocytoma cells</topic><topic>Plant extracts</topic><topic>Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tusi, Solaleh Khoramian</creatorcontrib><creatorcontrib>Khodagholi, Fariba</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cytotechnology (Dordrecht)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tusi, Solaleh Khoramian</au><au>Khodagholi, Fariba</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Salvia macilenta exhibits antiglycating activity and protects PC12 cells against H2O2-induced apoptosis</atitle><jtitle>Cytotechnology (Dordrecht)</jtitle><stitle>Cytotechnology</stitle><date>2014-01-01</date><risdate>2014</risdate><volume>66</volume><issue>1</issue><spage>169</spage><epage>179</epage><pages>169-179</pages><issn>0920-9069</issn><eissn>1573-0778</eissn><abstract>Salvia macilenta
is a member of the genus
Salvia
(Laminaceae) whose antioxidant activity and neuroprotective effect has been shown previously. The present study aimed to examine the antiglycating and antiapoptotic abilities of methanolic extract of this plant. Moreover, the effect of
S. macilenta
on neurite outgrowth and complexity after exposure to H
2
O
2
has been studied. Base on our results,
S. macilenta
has antiglycating activity and protects PC12 cells against oxidative stress-induced apoptotic cell death, as examined by Hoechst staining and Western blot analysis of caspase-3, Bax, Bcl-2 and PARP. We further showed that
S. macilenta
decreased neurite growth and complexity impairment in differentiated PC12 cells exposed to oxidative stress. It caused a decrease in cell body area, neurite width, and the proportion of bipolar cells, while significantly increasing neurite length, the number of primary neurites per cell and the ratio of nodes to primary neuritis. All around, the mentioned results open a new horizon for future works to use this plant as a potential neuroprotective agent.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>23579246</pmid><doi>10.1007/s10616-013-9550-x</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Cytotechnology (Dordrecht), 2014-01, Vol.66 (1), p.169-179 |
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| source | EZB-FREE-00999 freely available EZB journals; ProQuest Central UK/Ireland; PubMed Central; SpringerLink Journals - AutoHoldings; ProQuest Central |
| subjects | Antioxidants Apoptosis Axonogenesis BAX protein Bcl-2 protein Biochemistry Biomedicine Biotechnology Bipolar cells Caspase-3 Cell body Cell death Cell differentiation Chemistry Chemistry and Materials Science Enzymes Flavonoids Hydrogen peroxide Neuritis Neuroprotection Original Research Oxidation Oxidative stress Penicillin Pheochromocytoma cells Plant extracts Proteins |
| title | Salvia macilenta exhibits antiglycating activity and protects PC12 cells against H2O2-induced apoptosis |
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