Importance of Toxin A, Toxin B, and CDT in Virulence of an Epidemic Clostridium difficile Strain
Clostridium difficile infection is the main cause of healthcareacquired diarrhea in the developed world. In addition to the main virulence factors toxin A and B, epidemic, PCR Ribotype 027 strains, such as R20291, produce a third toxin, CDT. To develop effective medical countermeasures, it is import...
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| Veröffentlicht in: | The Journal of infectious diseases 2014-01, Vol.209 (1), p.83-86 |
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| container_title | The Journal of infectious diseases |
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| creator | Kuehne, Sarah A. Collery, Mark M. Kelly, Michelle L. Cartman, Stephen T. Cockayne, Alan Minton, Nigel P. |
| description | Clostridium difficile infection is the main cause of healthcareacquired diarrhea in the developed world. In addition to the main virulence factors toxin A and B, epidemic, PCR Ribotype 027 strains, such as R20291, produce a third toxin, CDT. To develop effective medical countermeasures, it is important to understand the importance of each toxin. Accordingly, we created all possible combinations of isogenic toxin mutants of R20291 and assessed their virulence. We demonstrated that either toxin A or toxin B alone can cause fulminant disease in the hamster infection model and present tantalizing data that C. difficile toxin may also contribute to virulence. |
| doi_str_mv | 10.1093/infdis/jit426 |
| format | Article |
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In addition to the main virulence factors toxin A and B, epidemic, PCR Ribotype 027 strains, such as R20291, produce a third toxin, CDT. To develop effective medical countermeasures, it is important to understand the importance of each toxin. Accordingly, we created all possible combinations of isogenic toxin mutants of R20291 and assessed their virulence. We demonstrated that either toxin A or toxin B alone can cause fulminant disease in the hamster infection model and present tantalizing data that C. difficile toxin may also contribute to virulence.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/jit426</identifier><identifier>PMID: 23935202</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>ADP Ribose Transferases - genetics ; ADP Ribose Transferases - physiology ; Animals ; BACTERIA ; Bacterial Proteins - genetics ; Bacterial Proteins - physiology ; Bacterial Toxins - genetics ; Bacteriology ; Biological and medical sciences ; Cell Death ; Clostridioides difficile - genetics ; Clostridioides difficile - pathogenicity ; Clostridium difficile ; Clostridium Infections - microbiology ; Clostridium Infections - pathology ; Cricetinae ; Cytotoxicity ; Disease models ; Enterotoxins - genetics ; Enterotoxins - physiology ; Epidemiology ; Female ; Fundamental and applied biological sciences. Psychology ; HT29 Cells ; Humans ; Infections ; Infectious diseases ; Major and Brief Reports ; Medical sciences ; Mesocricetus ; Microbiology ; Miscellaneous ; Polymerase chain reaction ; Ribotyping ; Toxins ; Vero cells ; Virulence ; Virulence - genetics ; Virulence - physiology</subject><ispartof>The Journal of infectious diseases, 2014-01, Vol.209 (1), p.83-86</ispartof><rights>Copyright © 2014 Oxford University Press on behalf of the Infectious Diseases Society of America</rights><rights>2015 INIST-CNRS</rights><rights>The Author 2013. 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In addition to the main virulence factors toxin A and B, epidemic, PCR Ribotype 027 strains, such as R20291, produce a third toxin, CDT. To develop effective medical countermeasures, it is important to understand the importance of each toxin. Accordingly, we created all possible combinations of isogenic toxin mutants of R20291 and assessed their virulence. We demonstrated that either toxin A or toxin B alone can cause fulminant disease in the hamster infection model and present tantalizing data that C. difficile toxin may also contribute to virulence.</description><subject>ADP Ribose Transferases - genetics</subject><subject>ADP Ribose Transferases - physiology</subject><subject>Animals</subject><subject>BACTERIA</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - physiology</subject><subject>Bacterial Toxins - genetics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cell Death</subject><subject>Clostridioides difficile - genetics</subject><subject>Clostridioides difficile - pathogenicity</subject><subject>Clostridium difficile</subject><subject>Clostridium Infections - microbiology</subject><subject>Clostridium Infections - pathology</subject><subject>Cricetinae</subject><subject>Cytotoxicity</subject><subject>Disease models</subject><subject>Enterotoxins - genetics</subject><subject>Enterotoxins - physiology</subject><subject>Epidemiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HT29 Cells</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Major and Brief Reports</subject><subject>Medical sciences</subject><subject>Mesocricetus</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Polymerase chain reaction</subject><subject>Ribotyping</subject><subject>Toxins</subject><subject>Vero cells</subject><subject>Virulence</subject><subject>Virulence - genetics</subject><subject>Virulence - physiology</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc2P1CAYh4nRuOPo0aOGi4mHrQu8QNuLyTquuskmHhy9IuVDmbQwQmv0v7eT1okeCJD34fcCD0JPKXlFSQtXIXobytUhjJzJe2hDBdSVlBTuow0hjFW0adsL9KiUAyGEg6wfogsGLQhG2AZ9vR2OKY86GoeTx_v0K0R8fbku3lxiHS3evd3jefcl5Kl3K6kjvjkG64Zg8K5PZczBhmnANngfTOgd_jRmHeJj9MDrvrgn67xFn9_d7HcfqruP729313eV4dCOlSSCC9FYZrxsnG18R5xjYDpuW6eJ8UQz4QE6X9uWN1a0nQFwzHVO2E5w2KLXS-5x6gZnjYtz-14dcxh0_q2SDur_Sgzf1bf0U0Ej-TzmgJdrQE4_JldGNYRiXN_r6NJUFOWyZbQ5fe0WVQtqciolO39uQ4k6WVGLFbVYmfnn_97tTP_VMAMvVkAXo3ufZx_z8TPXUACQp0c-W7hDGVM-1znUpOYE4A8c56I7</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Kuehne, Sarah A.</creator><creator>Collery, Mark M.</creator><creator>Kelly, Michelle L.</creator><creator>Cartman, Stephen T.</creator><creator>Cockayne, Alan</creator><creator>Minton, Nigel P.</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140101</creationdate><title>Importance of Toxin A, Toxin B, and CDT in Virulence of an Epidemic Clostridium difficile Strain</title><author>Kuehne, Sarah A. ; Collery, Mark M. ; Kelly, Michelle L. ; Cartman, Stephen T. ; Cockayne, Alan ; Minton, Nigel P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-6054558d2cf68ed8fb0ee23cb4d9ea0cf0a25f33bf7d948d59bc33e2ebe5db543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>ADP Ribose Transferases - genetics</topic><topic>ADP Ribose Transferases - physiology</topic><topic>Animals</topic><topic>BACTERIA</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - physiology</topic><topic>Bacterial Toxins - genetics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cell Death</topic><topic>Clostridioides difficile - genetics</topic><topic>Clostridioides difficile - pathogenicity</topic><topic>Clostridium difficile</topic><topic>Clostridium Infections - microbiology</topic><topic>Clostridium Infections - pathology</topic><topic>Cricetinae</topic><topic>Cytotoxicity</topic><topic>Disease models</topic><topic>Enterotoxins - genetics</topic><topic>Enterotoxins - physiology</topic><topic>Epidemiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HT29 Cells</topic><topic>Humans</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Major and Brief Reports</topic><topic>Medical sciences</topic><topic>Mesocricetus</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Polymerase chain reaction</topic><topic>Ribotyping</topic><topic>Toxins</topic><topic>Vero cells</topic><topic>Virulence</topic><topic>Virulence - genetics</topic><topic>Virulence - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuehne, Sarah A.</creatorcontrib><creatorcontrib>Collery, Mark M.</creatorcontrib><creatorcontrib>Kelly, Michelle L.</creatorcontrib><creatorcontrib>Cartman, Stephen T.</creatorcontrib><creatorcontrib>Cockayne, Alan</creatorcontrib><creatorcontrib>Minton, Nigel P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuehne, Sarah A.</au><au>Collery, Mark M.</au><au>Kelly, Michelle L.</au><au>Cartman, Stephen T.</au><au>Cockayne, Alan</au><au>Minton, Nigel P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Importance of Toxin A, Toxin B, and CDT in Virulence of an Epidemic Clostridium difficile Strain</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>209</volume><issue>1</issue><spage>83</spage><epage>86</epage><pages>83-86</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Clostridium difficile infection is the main cause of healthcareacquired diarrhea in the developed world. In addition to the main virulence factors toxin A and B, epidemic, PCR Ribotype 027 strains, such as R20291, produce a third toxin, CDT. To develop effective medical countermeasures, it is important to understand the importance of each toxin. Accordingly, we created all possible combinations of isogenic toxin mutants of R20291 and assessed their virulence. We demonstrated that either toxin A or toxin B alone can cause fulminant disease in the hamster infection model and present tantalizing data that C. difficile toxin may also contribute to virulence.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>23935202</pmid><doi>10.1093/infdis/jit426</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of infectious diseases, 2014-01, Vol.209 (1), p.83-86 |
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| source | MEDLINE; Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | ADP Ribose Transferases - genetics ADP Ribose Transferases - physiology Animals BACTERIA Bacterial Proteins - genetics Bacterial Proteins - physiology Bacterial Toxins - genetics Bacteriology Biological and medical sciences Cell Death Clostridioides difficile - genetics Clostridioides difficile - pathogenicity Clostridium difficile Clostridium Infections - microbiology Clostridium Infections - pathology Cricetinae Cytotoxicity Disease models Enterotoxins - genetics Enterotoxins - physiology Epidemiology Female Fundamental and applied biological sciences. Psychology HT29 Cells Humans Infections Infectious diseases Major and Brief Reports Medical sciences Mesocricetus Microbiology Miscellaneous Polymerase chain reaction Ribotyping Toxins Vero cells Virulence Virulence - genetics Virulence - physiology |
| title | Importance of Toxin A, Toxin B, and CDT in Virulence of an Epidemic Clostridium difficile Strain |
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