Expression and Site-Specific Mutagenesis of the Poliovirus 3C Protease in Escherichia coli

We have engineered a segment of the poliovirus genome (nucleotides 5438-6061) that encodes the 183 amino acid residues of the 3C region and 25 residues of the 3D region of the viral polyprotein into an Escherichia coli expression vector. The 3C region is a virus-specific protease, which, when expres...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1986-08, Vol.83 (15), p.5392-5396
Hauptverfasser:	Ivanoff, Lucinda A., Towatari, Takae, Ray, Jasodhara, Korant, Bruce D., Petteway, Stephen R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Active sites Amino acids Biological and medical sciences Biotechnology Cloning, Molecular Codons DNA DNA - genetics DNA, Viral - genetics Enzyme Precursors - genetics Enzymes Escherichia coli Fundamental and applied biological sciences. Psychology Genetic engineering Genetic mutation Genetic technics Genetic Vectors HeLa cells Methods. Procedures. Technologies Molecular cloning Mutation Peptide Hydrolases - genetics Poliovirus Poliovirus - enzymology Poliovirus - genetics Protein precursors Protein Processing, Post-Translational Proteins Structure-Activity Relationship Viral Proteins - genetics
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