A comparison and optimization of methods and factors affecting the transformation of Escherichia coli

DNA manipulation routinely requires competent bacteria that can be made using one of numerous methods. To determine the best methods, we compared four commonly used chemical methods (DMSO, MgCl2-CaCl2, CaCl2 and Hanahan's methods) on frequently used Escherichia coli (E. coli) strains: DH5α, XL-...

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Veröffentlicht in:	Bioscience reports 2013-12, Vol.33 (6)
Hauptverfasser:	Chan, Weng-Tat, Verma, Chandra S, Lane, David P, Gan, Samuel Ken-En
Format:	Artikel
Sprache:	eng
Schlagworte:	Calcium Chloride - chemistry Cell Membrane Permeability Culture Media Dimethyl Sulfoxide - chemistry DNA Transformation Competence Escherichia coli - genetics Magnesium Chloride - chemistry Original Paper Plasmids - genetics Transformation, Bacterial
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