A comparison and optimization of methods and factors affecting the transformation of Escherichia coli

A comparison and optimization of methods and factors affecting the transformation of Escherichia coli

DNA manipulation routinely requires competent bacteria that can be made using one of numerous methods. To determine the best methods, we compared four commonly used chemical methods (DMSO, MgCl2-CaCl2, CaCl2 and Hanahan's methods) on frequently used Escherichia coli (E. coli) strains: DH5α, XL-...

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Veröffentlicht in:Bioscience reports 2013-12, Vol.33 (6)
Hauptverfasser: Chan, Weng-Tat, Verma, Chandra S, Lane, David P, Gan, Samuel Ken-En
Format: Artikel
Sprache:eng
Schlagworte:
Calcium Chloride - chemistry
Cell Membrane Permeability
Culture Media
Dimethyl Sulfoxide - chemistry
DNA Transformation Competence
Escherichia coli - genetics
Magnesium Chloride - chemistry
Original Paper
Plasmids - genetics
Transformation, Bacterial
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Zusammenfassung:DNA manipulation routinely requires competent bacteria that can be made using one of numerous methods. To determine the best methods, we compared four commonly used chemical methods (DMSO, MgCl2-CaCl2, CaCl2 and Hanahan's methods) on frequently used Escherichia coli (E. coli) strains: DH5α, XL-1 Blue, SCS110, JM109, TOP10 and BL21-(DE3)-PLysS. Hanahan's method was found to be most effective for DH5α, XL-1 Blue and JM109 strains (P
ISSN:0144-8463
1573-4935
DOI:10.1042/BSR20130098