Rapid and Cost-Effective Gene Targeting in Rat Embryonic Stem Cells by TALENs

The rat is the preferred animal model in many areas of biomedical research and drug development. Genetic manipulation in rats has lagged behind that in mice due to the lack of efficient gene targeting tools. Previously, we generated a knockout rat via conventional homologous recombination in rat emb...

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Veröffentlicht in:	Journal of genetics and genomics 2012-06, Vol.39 (6), p.275-280
Hauptverfasser:	Tong, Chang, Huang, Guanyi, Ashton, Charles, Wu, Hongping, Yan, Hexin, Ying, Qi-Long
Format:	Artikel
Sprache:	eng
Schlagworte:	animal models Animals Base Sequence biomedical research BMPR2 Cell Line DNA damage DNA双链断裂 drugs embryonic stem cells Embryonic Stem Cells - metabolism Gene Order Gene Targeting genes genetic engineering Genetic Vectors homologous recombination metabolism Mice Molecular Sequence Data polymerase chain reaction Rat ES cells Rats Recombination, Genetic stems TAL effector transcription (genetics) transfection 动物模型基因定位基因打靶技术成本效益胚胎干细胞系药物开发转基因老鼠
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