Caries arresting effect of silver diamine fluoride on dentine carious lesion with S. mutans and L. acidophilus dual-species cariogenic biofilm

This in vitro study investigated the effects of silver diamine fluoride (SDF) on dentine carious lesion with cariogenic biofilm. Thirty human dentine blocks were inoculated with Streptococcus mutans and Lactobacillus acidophilus dual-species biofilm to create carious lesion. They were equally divide...

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Veröffentlicht in:Medicina oral, patología oral y cirugía bucal patología oral y cirugía bucal, 2013-11, Vol.18 (6), p.e824-e831
Hauptverfasser: Mei, May-Lei, Chu, Chun-Hung, Low, Kan-Hung, Che, Ching-Ming, Lo, Edward-Chin-Man
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creator Mei, May-Lei
Chu, Chun-Hung
Low, Kan-Hung
Che, Ching-Ming
Lo, Edward-Chin-Man
description This in vitro study investigated the effects of silver diamine fluoride (SDF) on dentine carious lesion with cariogenic biofilm. Thirty human dentine blocks were inoculated with Streptococcus mutans and Lactobacillus acidophilus dual-species biofilm to create carious lesion. They were equally divided into test and control group to receive topical application of SDF and water. After incubation anaerobically using micro-well plate at 37oC for 7 days, the biofilms were evaluated for kinetics, morphology and viability by colony forming units (CFU), scanning electron microscopy (SEM), and confocal microscopy (CLSM), respectively. The carious lesion underwent crystal characteristics analysis, evaluation of the changes in chemical structure and density of collagen fibrils using x-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and immune-labeling. The log CFU of S. mutans and L. acidophilus in the test group was significantly lower than control group. SEM and CLSM showed confluent biofilm in control group, but not in test group. XRD showed the loss of crystallinity of dentine due to the dissolution of hydroxyapatite crystal structure in test group was less than control group. FTIR showed that log [Amide I: HPO42-] for test vs. control group was 0.31±0.10 vs. 0.57±0.13 (p
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Thirty human dentine blocks were inoculated with Streptococcus mutans and Lactobacillus acidophilus dual-species biofilm to create carious lesion. They were equally divided into test and control group to receive topical application of SDF and water. After incubation anaerobically using micro-well plate at 37oC for 7 days, the biofilms were evaluated for kinetics, morphology and viability by colony forming units (CFU), scanning electron microscopy (SEM), and confocal microscopy (CLSM), respectively. The carious lesion underwent crystal characteristics analysis, evaluation of the changes in chemical structure and density of collagen fibrils using x-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and immune-labeling. The log CFU of S. mutans and L. acidophilus in the test group was significantly lower than control group. SEM and CLSM showed confluent biofilm in control group, but not in test group. XRD showed the loss of crystallinity of dentine due to the dissolution of hydroxyapatite crystal structure in test group was less than control group. FTIR showed that log [Amide I: HPO42-] for test vs. control group was 0.31±0.10 vs. 0.57±0.13 (p&lt;0.05). The gold-labeling density in test vs. control group was 8.54±2.44/µm2 vs. 12.91±4.24/µm2 (p=0.04). 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Thirty human dentine blocks were inoculated with Streptococcus mutans and Lactobacillus acidophilus dual-species biofilm to create carious lesion. They were equally divided into test and control group to receive topical application of SDF and water. After incubation anaerobically using micro-well plate at 37oC for 7 days, the biofilms were evaluated for kinetics, morphology and viability by colony forming units (CFU), scanning electron microscopy (SEM), and confocal microscopy (CLSM), respectively. The carious lesion underwent crystal characteristics analysis, evaluation of the changes in chemical structure and density of collagen fibrils using x-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and immune-labeling. The log CFU of S. mutans and L. acidophilus in the test group was significantly lower than control group. SEM and CLSM showed confluent biofilm in control group, but not in test group. XRD showed the loss of crystallinity of dentine due to the dissolution of hydroxyapatite crystal structure in test group was less than control group. FTIR showed that log [Amide I: HPO42-] for test vs. control group was 0.31±0.10 vs. 0.57±0.13 (p&lt;0.05). The gold-labeling density in test vs. control group was 8.54±2.44/µm2 vs. 12.91±4.24/µm2 (p=0.04). SDF had antimicrobial activity against the cariogenic biofilms and reduced demineralization of dentine.</description><subject>Biofilms - drug effects</subject><subject>Dental Caries - drug therapy</subject><subject>Dentistry</subject><subject>Fluorides, Topical</subject><subject>Humans</subject><subject>Lactobacillus acidophilus - drug effects</subject><subject>Lactobacillus acidophilus - physiology</subject><subject>Oral Medicine and Pathology</subject><subject>Quaternary Ammonium Compounds - therapeutic use</subject><subject>Silver Compounds</subject><subject>Streptococcus mutans - drug effects</subject><subject>Streptococcus mutans - physiology</subject><issn>1698-6946</issn><issn>1698-4447</issn><issn>1698-6946</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUuPFCEUhYnROA9dujUs3VTLqwpqY2I6vpJOXKhrQsGlG0NBC1Vj5k_4m6Vn2sm4gly-e-7lHIReUbIRnMq3M7hcTNxQpTh9gi7pMKpuGMXw9NH9Al3V-pMQLqkcnqMLxiVjlNNL9GdrSoCKTSlQl5D2GLwHu-DscQ3xBgp2wcwhAfZxzSU4wDlhB2k51WzrzmvFEWpo5d9hOeBvGzyvi0lNNDm822Bjg8vHQ4gNdKuJXT2CPQ29695DChZPIfsQ5xfomTexwsvzeY1-fPzwffu523399GX7ftdZruTSWQJcCTXJnrFBUD95CgM3EzDhGDO9NcPEFVPS9ZKYceq9EZ4wPypnpRPAr9G7e93jOjUHbftOM1EfS5hNudXZBP3_SwoHvc83mqteEMmawJuzQMm_1madnkO1EKNJ0AzRVIiRKUoG1dDuHrUl11rAP4yhRJ8y1OcM9V2GjX_9eLcH-l9o_C8olZ14</recordid><startdate>20131101</startdate><enddate>20131101</enddate><creator>Mei, May-Lei</creator><creator>Chu, Chun-Hung</creator><creator>Low, Kan-Hung</creator><creator>Che, Ching-Ming</creator><creator>Lo, Edward-Chin-Man</creator><general>Medicina Oral S.L</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20131101</creationdate><title>Caries arresting effect of silver diamine fluoride on dentine carious lesion with S. mutans and L. acidophilus dual-species cariogenic biofilm</title><author>Mei, May-Lei ; Chu, Chun-Hung ; Low, Kan-Hung ; Che, Ching-Ming ; Lo, Edward-Chin-Man</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-c0e3848b7522641fbf1e63abe24d22a5ca6b38287d570a9b5fa4f02f98dc7d4e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Biofilms - drug effects</topic><topic>Dental Caries - drug therapy</topic><topic>Dentistry</topic><topic>Fluorides, Topical</topic><topic>Humans</topic><topic>Lactobacillus acidophilus - drug effects</topic><topic>Lactobacillus acidophilus - physiology</topic><topic>Oral Medicine and Pathology</topic><topic>Quaternary Ammonium Compounds - therapeutic use</topic><topic>Silver Compounds</topic><topic>Streptococcus mutans - drug effects</topic><topic>Streptococcus mutans - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mei, May-Lei</creatorcontrib><creatorcontrib>Chu, Chun-Hung</creatorcontrib><creatorcontrib>Low, Kan-Hung</creatorcontrib><creatorcontrib>Che, Ching-Ming</creatorcontrib><creatorcontrib>Lo, Edward-Chin-Man</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Medicina oral, patología oral y cirugía bucal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mei, May-Lei</au><au>Chu, Chun-Hung</au><au>Low, Kan-Hung</au><au>Che, Ching-Ming</au><au>Lo, Edward-Chin-Man</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Caries arresting effect of silver diamine fluoride on dentine carious lesion with S. mutans and L. acidophilus dual-species cariogenic biofilm</atitle><jtitle>Medicina oral, patología oral y cirugía bucal</jtitle><addtitle>Med Oral Patol Oral Cir Bucal</addtitle><date>2013-11-01</date><risdate>2013</risdate><volume>18</volume><issue>6</issue><spage>e824</spage><epage>e831</epage><pages>e824-e831</pages><issn>1698-6946</issn><issn>1698-4447</issn><eissn>1698-6946</eissn><abstract>This in vitro study investigated the effects of silver diamine fluoride (SDF) on dentine carious lesion with cariogenic biofilm. Thirty human dentine blocks were inoculated with Streptococcus mutans and Lactobacillus acidophilus dual-species biofilm to create carious lesion. They were equally divided into test and control group to receive topical application of SDF and water. After incubation anaerobically using micro-well plate at 37oC for 7 days, the biofilms were evaluated for kinetics, morphology and viability by colony forming units (CFU), scanning electron microscopy (SEM), and confocal microscopy (CLSM), respectively. The carious lesion underwent crystal characteristics analysis, evaluation of the changes in chemical structure and density of collagen fibrils using x-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and immune-labeling. The log CFU of S. mutans and L. acidophilus in the test group was significantly lower than control group. SEM and CLSM showed confluent biofilm in control group, but not in test group. 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subjects Biofilms - drug effects
Dental Caries - drug therapy
Dentistry
Fluorides, Topical
Humans
Lactobacillus acidophilus - drug effects
Lactobacillus acidophilus - physiology
Oral Medicine and Pathology
Quaternary Ammonium Compounds - therapeutic use
Silver Compounds
Streptococcus mutans - drug effects
Streptococcus mutans - physiology
title Caries arresting effect of silver diamine fluoride on dentine carious lesion with S. mutans and L. acidophilus dual-species cariogenic biofilm
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