Expression of VPAC1 in a murine model of allergic asthma
Vasoactive intestinal polypeptide (VIP) is a putative neurotransmitter of the inhibitory non-adrenergic non-cholinergic nervous system and influences the mammalian airway function in various ways. Hence known for bronchodilatory, immunomodulatory and mucus secretion modulating effects by interacting...
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| Veröffentlicht in: | Journal of occupational medicine and toxicology (London, England) England), 2013-10, Vol.8 (1), p.28-28, Article 28 |
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| creator | Lauenstein, Hans D Quarcoo, David Welte, Tobias Braun, Armin Groneberg, David A |
| description | Vasoactive intestinal polypeptide (VIP) is a putative neurotransmitter of the inhibitory non-adrenergic non-cholinergic nervous system and influences the mammalian airway function in various ways. Hence known for bronchodilatory, immunomodulatory and mucus secretion modulating effects by interacting with the VIP receptors VPAC1 and VPAC2, it is discussed to be a promising target for pharmaceutical intervention in common diseases such as COPD and bronchial asthma. Here we examined the expression and transcriptional regulation of VPAC1 in the lungs of allergic mice using an ovalbumin (OVA) -induced model of allergic asthma. Mice were sensitized to OVA and challenged with an OVA aerosol. In parallel a control group was sham sensitized with saline. VPAC1 expression was examined using RT-PCR and real time-PCR studies were performed to quantify gene transcription. VPAC1 mRNA expression was detected in all samples of OVA-sensitized and challenged animals and control tissues. Further realtime analysis did not show significant differences at the transcriptional level.Although the present studies did not indicate a major transcriptional regulation of VPAC1 in states of allergic airway inflammation, immunomodulatory effects of VPAC1 might still be present due to regulations at the translational level. |
| doi_str_mv | 10.1186/1745-6673-8-28 |
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Hence known for bronchodilatory, immunomodulatory and mucus secretion modulating effects by interacting with the VIP receptors VPAC1 and VPAC2, it is discussed to be a promising target for pharmaceutical intervention in common diseases such as COPD and bronchial asthma. Here we examined the expression and transcriptional regulation of VPAC1 in the lungs of allergic mice using an ovalbumin (OVA) -induced model of allergic asthma. Mice were sensitized to OVA and challenged with an OVA aerosol. In parallel a control group was sham sensitized with saline. VPAC1 expression was examined using RT-PCR and real time-PCR studies were performed to quantify gene transcription. VPAC1 mRNA expression was detected in all samples of OVA-sensitized and challenged animals and control tissues. Further realtime analysis did not show significant differences at the transcriptional level.Although the present studies did not indicate a major transcriptional regulation of VPAC1 in states of allergic airway inflammation, immunomodulatory effects of VPAC1 might still be present due to regulations at the translational level.</description><identifier>ISSN: 1745-6673</identifier><identifier>EISSN: 1745-6673</identifier><identifier>DOI: 10.1186/1745-6673-8-28</identifier><identifier>PMID: 24107483</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Albumin ; Asthma ; B cells ; Cell receptors ; Deoxyribonucleic acid ; Disease ; DNA ; Gene expression ; Genetic aspects ; Lymphocytes ; Medicine ; Occupational diseases ; RNA ; Rodents</subject><ispartof>Journal of occupational medicine and toxicology (London, England), 2013-10, Vol.8 (1), p.28-28, Article 28</ispartof><rights>COPYRIGHT 2013 BioMed Central Ltd.</rights><rights>2013 Lauenstein et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2013 Lauenstein et al.; licensee BioMed Central Ltd. 2013 Lauenstein et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b576t-cdd4e9fdd503971a66194e4c86c8809db2ec2b6af77fc141a1e8226770bcfe5d3</citedby><cites>FETCH-LOGICAL-b576t-cdd4e9fdd503971a66194e4c86c8809db2ec2b6af77fc141a1e8226770bcfe5d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852716/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852716/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24107483$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lauenstein, Hans D</creatorcontrib><creatorcontrib>Quarcoo, David</creatorcontrib><creatorcontrib>Welte, Tobias</creatorcontrib><creatorcontrib>Braun, Armin</creatorcontrib><creatorcontrib>Groneberg, David A</creatorcontrib><title>Expression of VPAC1 in a murine model of allergic asthma</title><title>Journal of occupational medicine and toxicology (London, England)</title><addtitle>J Occup Med Toxicol</addtitle><description>Vasoactive intestinal polypeptide (VIP) is a putative neurotransmitter of the inhibitory non-adrenergic non-cholinergic nervous system and influences the mammalian airway function in various ways. Hence known for bronchodilatory, immunomodulatory and mucus secretion modulating effects by interacting with the VIP receptors VPAC1 and VPAC2, it is discussed to be a promising target for pharmaceutical intervention in common diseases such as COPD and bronchial asthma. Here we examined the expression and transcriptional regulation of VPAC1 in the lungs of allergic mice using an ovalbumin (OVA) -induced model of allergic asthma. Mice were sensitized to OVA and challenged with an OVA aerosol. In parallel a control group was sham sensitized with saline. VPAC1 expression was examined using RT-PCR and real time-PCR studies were performed to quantify gene transcription. VPAC1 mRNA expression was detected in all samples of OVA-sensitized and challenged animals and control tissues. Further realtime analysis did not show significant differences at the transcriptional level.Although the present studies did not indicate a major transcriptional regulation of VPAC1 in states of allergic airway inflammation, immunomodulatory effects of VPAC1 might still be present due to regulations at the translational level.</description><subject>Albumin</subject><subject>Asthma</subject><subject>B cells</subject><subject>Cell receptors</subject><subject>Deoxyribonucleic acid</subject><subject>Disease</subject><subject>DNA</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Lymphocytes</subject><subject>Medicine</subject><subject>Occupational diseases</subject><subject>RNA</subject><subject>Rodents</subject><issn>1745-6673</issn><issn>1745-6673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1ks9rFTEQx4MotlavHmXBi5et-Z28i_B4tCoU9KBeQzaZvKbsJs_kreh_3yytz1YrOSTMfPKdmS-D0EuCTwnR8i1RXPRSKtbrnupH6PgQeHznfYSe1XqFMRcCs6foiHKCFdfsGOmzn7sCtcacuhy6b5_XG9LF1NlumktM0E3Zw7ik7DhC2UbX2bq_nOxz9CTYscKL2_sEfT0_-7L50F98ev9xs77oB6Hkvnfec1gF71vhlSJWSrLiwJ2WTmu88gMFRwdpg1LBEU4sAU2pVAoPLoDw7AS9u9HdzcME3kHaFzuaXYmTLb9MttHcz6R4abb5h2FaUEVkE1jfCAwx_0fgfsblySzOmcU5ow3VTePNbRMlf5-h7s0Uq4NxtAnyXA3hQgtGJFcNff0XepXnkppFjeJMCKYY_UNt7QgmppBbabeImrVgXGEhBG_U6QNUOx6m6HKCEFv8oQ-u5FoLhMOYBJtlX_4d7NVddw_47wVh19auuO8</recordid><startdate>20131010</startdate><enddate>20131010</enddate><creator>Lauenstein, Hans D</creator><creator>Quarcoo, David</creator><creator>Welte, Tobias</creator><creator>Braun, Armin</creator><creator>Groneberg, David A</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T2</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7U2</scope><scope>5PM</scope></search><sort><creationdate>20131010</creationdate><title>Expression of VPAC1 in a murine model of allergic asthma</title><author>Lauenstein, Hans D ; Quarcoo, David ; Welte, Tobias ; Braun, Armin ; Groneberg, David A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b576t-cdd4e9fdd503971a66194e4c86c8809db2ec2b6af77fc141a1e8226770bcfe5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Albumin</topic><topic>Asthma</topic><topic>B cells</topic><topic>Cell receptors</topic><topic>Deoxyribonucleic acid</topic><topic>Disease</topic><topic>DNA</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Lymphocytes</topic><topic>Medicine</topic><topic>Occupational diseases</topic><topic>RNA</topic><topic>Rodents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lauenstein, Hans D</creatorcontrib><creatorcontrib>Quarcoo, David</creatorcontrib><creatorcontrib>Welte, Tobias</creatorcontrib><creatorcontrib>Braun, Armin</creatorcontrib><creatorcontrib>Groneberg, David A</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Public Health Database (Proquest)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Safety Science and Risk</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of occupational medicine and toxicology (London, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lauenstein, Hans D</au><au>Quarcoo, David</au><au>Welte, Tobias</au><au>Braun, Armin</au><au>Groneberg, David A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of VPAC1 in a murine model of allergic asthma</atitle><jtitle>Journal of occupational medicine and toxicology (London, England)</jtitle><addtitle>J Occup Med Toxicol</addtitle><date>2013-10-10</date><risdate>2013</risdate><volume>8</volume><issue>1</issue><spage>28</spage><epage>28</epage><pages>28-28</pages><artnum>28</artnum><issn>1745-6673</issn><eissn>1745-6673</eissn><abstract>Vasoactive intestinal polypeptide (VIP) is a putative neurotransmitter of the inhibitory non-adrenergic non-cholinergic nervous system and influences the mammalian airway function in various ways. Hence known for bronchodilatory, immunomodulatory and mucus secretion modulating effects by interacting with the VIP receptors VPAC1 and VPAC2, it is discussed to be a promising target for pharmaceutical intervention in common diseases such as COPD and bronchial asthma. Here we examined the expression and transcriptional regulation of VPAC1 in the lungs of allergic mice using an ovalbumin (OVA) -induced model of allergic asthma. Mice were sensitized to OVA and challenged with an OVA aerosol. In parallel a control group was sham sensitized with saline. VPAC1 expression was examined using RT-PCR and real time-PCR studies were performed to quantify gene transcription. VPAC1 mRNA expression was detected in all samples of OVA-sensitized and challenged animals and control tissues. 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| source | Open Access: PubMed Central; DOAJ Directory of Open Access Journals; SpringerOpen (Open Access); Free E-Journal (出版社公開部分のみ）; Springer Online Journals - JUSTICE; PubMed Central Open Access |
| subjects | Albumin Asthma B cells Cell receptors Deoxyribonucleic acid Disease DNA Gene expression Genetic aspects Lymphocytes Medicine Occupational diseases RNA Rodents |
| title | Expression of VPAC1 in a murine model of allergic asthma |
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