Proteomic patterns of nipple aspirate fluids obtained by SELDI-TOF: potential for new biomarkers to aid in the diagnosis of breast cancer
Nipple aspirate fluid (NAF) has been used for many years as a potential non-invasive method to identify markers for breast cancer risk or early detection. Because individual markers have not been optimal, we are exploring the use of surface enhanced laser desorption and ionization time of flight (SE...
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| Veröffentlicht in: | Disease markers 2001, Vol.17 (4), p.301-307 |
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| creator | Paweletz, C P Trock, B Pennanen, M Tsangaris, T Magnant, C Liotta, L A Petricoin, 3rd, E F |
| description | Nipple aspirate fluid (NAF) has been used for many years as a potential non-invasive method to identify markers for breast cancer risk or early detection. Because individual markers have not been optimal, we are exploring the use of surface enhanced laser desorption and ionization time of flight (SELDI-TOF) mass spectrometry to identify patterns of proteins that might define a proteomic signature for breast cancer. SELDI-TOF was used to analyze a study set of NAF samples that included 12 women with breast cancer and 15 healthy controls (the latter included three women with an abnormal mammogram but subsequent normal biopsy). In this preliminary report, we present data showing that SELDI analysis of NAF is rapid, reproducible, and capable of identifying protein signatures that appear to differentiate NAF samples from breast cancer patients and healthy controls, including those with an abnormal mammogram who were later proven to be biopsy normal. |
| doi_str_mv | 10.1155/2001/674959 |
| format | Article |
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In this preliminary report, we present data showing that SELDI analysis of NAF is rapid, reproducible, and capable of identifying protein signatures that appear to differentiate NAF samples from breast cancer patients and healthy controls, including those with an abnormal mammogram who were later proven to be biopsy normal.</description><subject>Biomarkers, Tumor - metabolism</subject><subject>Body Fluids - metabolism</subject><subject>Breast Neoplasms - diagnosis</subject><subject>Breast Neoplasms - metabolism</subject><subject>Drainage</subject><subject>Female</subject><subject>Humans</subject><subject>Lasers</subject><subject>Nipples - metabolism</subject><subject>Other</subject><subject>Proteome - metabolism</subject><subject>Reproducibility of Results</subject><subject>Time Factors</subject><issn>0278-0240</issn><issn>1875-8630</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU1PVDEYhRuigRFZsTdduTFX2t7bacuChCAoySSYiOumH--Fyp320nY0_AT_tcWZ-LF6F-fJOSfvQeiYkveUcn7CCKEnSzEorvbQgkrBO7nsyQu0IEzIjrCBHKBXpXxrHFOD2kcHlApFpBIL9PNzThXSOjg8m1ohx4LTiGOY5wmwKXPIpgIep03wTbHVhAge2yf85XL14bq7vbk6xXOziDWYCY8p4wg_sA1pbfID5IJrwiZ4HCKu94B9MHcxlfA7xWYwpWJnooP8Gr0czVTgaHcP0dery9uLT93q5uP1xfmqc70QtXNCeG8sAa-AW2_ADhIIk0RRYa3lXAqmbM-UA0Y994aPcmmJkHwQS8ugP0RnW995Y9fgXWuezaTnHFrjJ51M0P8rMdzru_Rd95JTovpm8HZnkNPjBkrV61AcTJOJkDZFi_bwngjRwHdb0OVUSobxTwgl-nk6_Tyd3k7X6Df_9vrL7rbqfwHFy5bf</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>Paweletz, C P</creator><creator>Trock, B</creator><creator>Pennanen, M</creator><creator>Tsangaris, T</creator><creator>Magnant, C</creator><creator>Liotta, L A</creator><creator>Petricoin, 3rd, E F</creator><general>IOS Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2001</creationdate><title>Proteomic patterns of nipple aspirate fluids obtained by SELDI-TOF: potential for new biomarkers to aid in the diagnosis of breast cancer</title><author>Paweletz, C P ; Trock, B ; Pennanen, M ; Tsangaris, T ; Magnant, C ; Liotta, L A ; Petricoin, 3rd, E F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-c77ddab0ed9e5bdaeb48e0280917bbb558729b329ce21d5da5f86b0785476b2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Biomarkers, Tumor - metabolism</topic><topic>Body Fluids - metabolism</topic><topic>Breast Neoplasms - diagnosis</topic><topic>Breast Neoplasms - metabolism</topic><topic>Drainage</topic><topic>Female</topic><topic>Humans</topic><topic>Lasers</topic><topic>Nipples - metabolism</topic><topic>Other</topic><topic>Proteome - metabolism</topic><topic>Reproducibility of Results</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Paweletz, C P</creatorcontrib><creatorcontrib>Trock, B</creatorcontrib><creatorcontrib>Pennanen, M</creatorcontrib><creatorcontrib>Tsangaris, T</creatorcontrib><creatorcontrib>Magnant, C</creatorcontrib><creatorcontrib>Liotta, L A</creatorcontrib><creatorcontrib>Petricoin, 3rd, E F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Disease markers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paweletz, C P</au><au>Trock, B</au><au>Pennanen, M</au><au>Tsangaris, T</au><au>Magnant, C</au><au>Liotta, L A</au><au>Petricoin, 3rd, E F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomic patterns of nipple aspirate fluids obtained by SELDI-TOF: potential for new biomarkers to aid in the diagnosis of breast cancer</atitle><jtitle>Disease markers</jtitle><addtitle>Dis Markers</addtitle><date>2001</date><risdate>2001</risdate><volume>17</volume><issue>4</issue><spage>301</spage><epage>307</epage><pages>301-307</pages><issn>0278-0240</issn><eissn>1875-8630</eissn><abstract>Nipple aspirate fluid (NAF) has been used for many years as a potential non-invasive method to identify markers for breast cancer risk or early detection. Because individual markers have not been optimal, we are exploring the use of surface enhanced laser desorption and ionization time of flight (SELDI-TOF) mass spectrometry to identify patterns of proteins that might define a proteomic signature for breast cancer. SELDI-TOF was used to analyze a study set of NAF samples that included 12 women with breast cancer and 15 healthy controls (the latter included three women with an abnormal mammogram but subsequent normal biopsy). In this preliminary report, we present data showing that SELDI analysis of NAF is rapid, reproducible, and capable of identifying protein signatures that appear to differentiate NAF samples from breast cancer patients and healthy controls, including those with an abnormal mammogram who were later proven to be biopsy normal.</abstract><cop>United States</cop><pub>IOS Press</pub><pmid>11790897</pmid><doi>10.1155/2001/674959</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Disease markers, 2001, Vol.17 (4), p.301-307 |
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| source | MEDLINE; Wiley Online Library Open Access; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Biomarkers, Tumor - metabolism Body Fluids - metabolism Breast Neoplasms - diagnosis Breast Neoplasms - metabolism Drainage Female Humans Lasers Nipples - metabolism Other Proteome - metabolism Reproducibility of Results Time Factors |
| title | Proteomic patterns of nipple aspirate fluids obtained by SELDI-TOF: potential for new biomarkers to aid in the diagnosis of breast cancer |
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