Contribution of flow cytometry to acute leukemia classification in Tunisia
The precision of immunological characterization of leukemias was improved by a certain number of technical innovations, particularly hybridoma production and standardization, resulting in monoclonal antibodies and definition of recognised cellular antigens (designated by CD: Cluster of Differentiati...
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| Veröffentlicht in: | Disease markers 2000, Vol.16 (3-4), p.131-133 |
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| creator | FEKI, S EL OMRI, H LAATIRI, M. A ENNABLI, S BOUKEF, K JENHANI, F |
| description | The precision of immunological characterization of leukemias was improved by a certain number of technical innovations, particularly hybridoma production and standardization, resulting in monoclonal antibodies and definition of recognised cellular antigens (designated by CD: Cluster of Differentiation). The aim of this work was to determine the immunophenotyping profile of patients with leukemia, by means of a flow cytometric method: 66 blood samples coming from leukemic persons in the Sahel region were studied by flow cytometry, using about thirty monoclonal antibodies all marked with a fluorochrome, in one or two colour systems to assess their distribution according to type (lymphoid B or T / myeloid) and age, and to search for possible co-expressions of markers of different lineages. The marked preponderance of childhood B-ALL in our series is, at least partly, attributable to the age distribution of the Tunisian population. In agreement with studies from other countries, the majority of AML cases occurred among adults. A high proportion of AML cases in our series co-expressed markers of other lineages. Overall, accurate classification of acute leukemias was possible from a simple peripheral blood sample in 62 of 66 cases (93.9%). |
| doi_str_mv | 10.1155/2000/953059 |
| format | Article |
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The marked preponderance of childhood B-ALL in our series is, at least partly, attributable to the age distribution of the Tunisian population. In agreement with studies from other countries, the majority of AML cases occurred among adults. A high proportion of AML cases in our series co-expressed markers of other lineages. Overall, accurate classification of acute leukemias was possible from a simple peripheral blood sample in 62 of 66 cases (93.9%).</description><identifier>ISSN: 0278-0240</identifier><identifier>EISSN: 1875-8630</identifier><identifier>DOI: 10.1155/2000/953059</identifier><identifier>PMID: 11381193</identifier><identifier>CODEN: DMARD3</identifier><language>eng</language><publisher>Amsterdam: IOS Press</publisher><subject>Acute Disease ; Adolescent ; Adult ; Age Factors ; Aged ; Antibodies, Monoclonal - immunology ; Antigens, CD - analysis ; Antigens, Neoplasm - analysis ; Antigens, Neoplasm - immunology ; Biological and medical sciences ; Cell Lineage ; Child ; Child, Preschool ; Female ; Flow Cytometry ; Fluorescent Dyes ; Hematologic and hematopoietic diseases ; Humans ; Immunophenotyping ; Infant ; Infant, Newborn ; Leukemia - classification ; Leukemia - pathology ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Male ; Medical sciences ; Middle Aged ; Neoplastic Stem Cells - chemistry ; Other ; Tropical medicine ; Tunisia</subject><ispartof>Disease markers, 2000, Vol.16 (3-4), p.131-133</ispartof><rights>2002 INIST-CNRS</rights><rights>Copyright © 2000 Hindawi Publishing Corporation. 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-a1237ddb88fc451d1c4d01b92cbe84f5e37f6788f65eb938c2a04af6bf038d643</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3850556/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3850556/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14140133$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11381193$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FEKI, S</creatorcontrib><creatorcontrib>EL OMRI, H</creatorcontrib><creatorcontrib>LAATIRI, M. A</creatorcontrib><creatorcontrib>ENNABLI, S</creatorcontrib><creatorcontrib>BOUKEF, K</creatorcontrib><creatorcontrib>JENHANI, F</creatorcontrib><title>Contribution of flow cytometry to acute leukemia classification in Tunisia</title><title>Disease markers</title><addtitle>Dis Markers</addtitle><description>The precision of immunological characterization of leukemias was improved by a certain number of technical innovations, particularly hybridoma production and standardization, resulting in monoclonal antibodies and definition of recognised cellular antigens (designated by CD: Cluster of Differentiation). The aim of this work was to determine the immunophenotyping profile of patients with leukemia, by means of a flow cytometric method: 66 blood samples coming from leukemic persons in the Sahel region were studied by flow cytometry, using about thirty monoclonal antibodies all marked with a fluorochrome, in one or two colour systems to assess their distribution according to type (lymphoid B or T / myeloid) and age, and to search for possible co-expressions of markers of different lineages. The marked preponderance of childhood B-ALL in our series is, at least partly, attributable to the age distribution of the Tunisian population. In agreement with studies from other countries, the majority of AML cases occurred among adults. A high proportion of AML cases in our series co-expressed markers of other lineages. Overall, accurate classification of acute leukemias was possible from a simple peripheral blood sample in 62 of 66 cases (93.9%).</description><subject>Acute Disease</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Age Factors</subject><subject>Aged</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens, CD - analysis</subject><subject>Antigens, Neoplasm - analysis</subject><subject>Antigens, Neoplasm - immunology</subject><subject>Biological and medical sciences</subject><subject>Cell Lineage</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Fluorescent Dyes</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>Immunophenotyping</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Leukemia - classification</subject><subject>Leukemia - pathology</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neoplastic Stem Cells - chemistry</subject><subject>Other</subject><subject>Tropical medicine</subject><subject>Tunisia</subject><issn>0278-0240</issn><issn>1875-8630</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1PwzAQxS0EoqUwsaMssKDQc2wnzoKEKj5ViaXMluPYYEjiEjug_vekNKIwMd3wfvd07x5CxxguMGZsmgDANGcEWL6DxphnLOYpgV00hiTjMSQURujA-1cAnOQ030cjjAnHOCdj9DBzTWht0QXrmsiZyFTuM1Kr4God2lUUXCRVF3RU6e5N11ZGqpLeW2OV_F6xTbToGuutPER7RlZeHw1zgp5urhezu3j-eHs_u5rHihIeYokTkpVlwblRlOESK1oCLvJEFZpTwzTJTJr1asp0kROuEglUmrQwQHiZUjJBlxvfZVfUulS6DyArsWxtLduVcNKKv0pjX8Sz-xCEM2As7Q3OBoPWvXfaB1Fbr3RVyUa7zossYTlQyP4F-w9SDnwNnm9A1TrvW21-rsEg1iWJdUliU1JPn_wOsGWHVnrgdACkV7IyrWyU9VuOYgqYEPIFY6-apQ</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>FEKI, S</creator><creator>EL OMRI, H</creator><creator>LAATIRI, M. A</creator><creator>ENNABLI, S</creator><creator>BOUKEF, K</creator><creator>JENHANI, F</creator><general>IOS Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2000</creationdate><title>Contribution of flow cytometry to acute leukemia classification in Tunisia</title><author>FEKI, S ; EL OMRI, H ; LAATIRI, M. A ; ENNABLI, S ; BOUKEF, K ; JENHANI, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-a1237ddb88fc451d1c4d01b92cbe84f5e37f6788f65eb938c2a04af6bf038d643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Acute Disease</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Age Factors</topic><topic>Aged</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens, CD - analysis</topic><topic>Antigens, Neoplasm - analysis</topic><topic>Antigens, Neoplasm - immunology</topic><topic>Biological and medical sciences</topic><topic>Cell Lineage</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Fluorescent Dyes</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>Immunophenotyping</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Leukemia - classification</topic><topic>Leukemia - pathology</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neoplastic Stem Cells - chemistry</topic><topic>Other</topic><topic>Tropical medicine</topic><topic>Tunisia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FEKI, S</creatorcontrib><creatorcontrib>EL OMRI, H</creatorcontrib><creatorcontrib>LAATIRI, M. 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The aim of this work was to determine the immunophenotyping profile of patients with leukemia, by means of a flow cytometric method: 66 blood samples coming from leukemic persons in the Sahel region were studied by flow cytometry, using about thirty monoclonal antibodies all marked with a fluorochrome, in one or two colour systems to assess their distribution according to type (lymphoid B or T / myeloid) and age, and to search for possible co-expressions of markers of different lineages. The marked preponderance of childhood B-ALL in our series is, at least partly, attributable to the age distribution of the Tunisian population. In agreement with studies from other countries, the majority of AML cases occurred among adults. A high proportion of AML cases in our series co-expressed markers of other lineages. 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| source | MEDLINE; Wiley Online Library Open Access; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Acute Disease Adolescent Adult Age Factors Aged Antibodies, Monoclonal - immunology Antigens, CD - analysis Antigens, Neoplasm - analysis Antigens, Neoplasm - immunology Biological and medical sciences Cell Lineage Child Child, Preschool Female Flow Cytometry Fluorescent Dyes Hematologic and hematopoietic diseases Humans Immunophenotyping Infant Infant, Newborn Leukemia - classification Leukemia - pathology Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Male Medical sciences Middle Aged Neoplastic Stem Cells - chemistry Other Tropical medicine Tunisia |
| title | Contribution of flow cytometry to acute leukemia classification in Tunisia |
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