Molecular characterisation of virus in the brains of patients with measles inclusion body encephalitis (MIBE)
BACKGROUND: During 2009/10 a major measles epidemic caused by genotype B3 occurred in South Africa. Measles inclusion body encephalitis (MIBE) was diagnosed in a number of highly immuno-compromised HIV patients. The diagnosis was based on typical clinical and MRI findings and positive measles virus...
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| Veröffentlicht in: | Virology journal 2013-09, Vol.10 (1), p.283-283, Article 283 |
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| description | BACKGROUND: During 2009/10 a major measles epidemic caused by genotype B3 occurred in South Africa. Measles inclusion body encephalitis (MIBE) was diagnosed in a number of highly immuno-compromised HIV patients. The diagnosis was based on typical clinical and MRI findings and positive measles virus PCR in brain or CSF. To characterize the brain virus, nucleoprotein, matrix, fusion and haemagglutinin genes from 4 cases was compared with virus from acutely infected patients. METHODS: cDNA was synthesized using random primers and viral genes were amplified by nested RT-PCR. PCR products were sequenced in the forward and reverse direction and a contig of each gene was created. Sequences were aligned with reference sequences from GenBank and other local sequences. RESULTS: Brain virus was very similar to the South African epidemic virus. Features characteristic of persistent measles virus in the brain were absent. Mutation frequency in brain virus was similar to epidemic virus and had the same substitution preference (U to C and C to U). The virus of 2 patients had the same L454W mutation in the fusion protein. CONCLUSION: The brain virus was very similar to the epidemic strain. The relatively few mutations probably reflect the short time from infection to brain disease in these highly immuno-compromised patients. |
| doi_str_mv | 10.1186/1743-422X-10-283 |
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Measles inclusion body encephalitis (MIBE) was diagnosed in a number of highly immuno-compromised HIV patients. The diagnosis was based on typical clinical and MRI findings and positive measles virus PCR in brain or CSF. To characterize the brain virus, nucleoprotein, matrix, fusion and haemagglutinin genes from 4 cases was compared with virus from acutely infected patients. METHODS: cDNA was synthesized using random primers and viral genes were amplified by nested RT-PCR. PCR products were sequenced in the forward and reverse direction and a contig of each gene was created. Sequences were aligned with reference sequences from GenBank and other local sequences. RESULTS: Brain virus was very similar to the South African epidemic virus. Features characteristic of persistent measles virus in the brain were absent. Mutation frequency in brain virus was similar to epidemic virus and had the same substitution preference (U to C and C to U). The virus of 2 patients had the same L454W mutation in the fusion protein. CONCLUSION: The brain virus was very similar to the epidemic strain. The relatively few mutations probably reflect the short time from infection to brain disease in these highly immuno-compromised patients.</description><identifier>ISSN: 1743-422X</identifier><identifier>EISSN: 1743-422X</identifier><identifier>DOI: 10.1186/1743-422X-10-283</identifier><identifier>PMID: 24025157</identifier><language>eng</language><publisher>England: Springer-Verlag</publisher><subject>Adolescent ; Adult ; Brain ; Brain - virology ; Brain research ; Children & youth ; Cluster Analysis ; Colleges & universities ; complementary DNA ; Complications and side effects ; encephalitis ; Female ; Genes ; Genetic aspects ; genetic databases ; genotype ; Genotype & phenotype ; Health aspects ; hemagglutinins ; HIV ; HIV (Viruses) ; HIV patients ; Human immunodeficiency virus ; Humans ; magnetic resonance imaging ; Male ; Measles ; Measles morbillivirus ; Measles virus ; Measles virus - genetics ; Measles virus - isolation & purification ; Molecular Sequence Data ; mutation ; Mutation Rate ; nucleoproteins ; Patients ; Phylogenetics ; Phylogeny ; Physiological aspects ; Point Mutation ; Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Viral - genetics ; Sequence Alignment ; Sequence Analysis, DNA ; South Africa ; Subacute sclerosing panencephalitis ; Subacute Sclerosing Panencephalitis - virology ; Trees ; Values ; Viral Proteins - genetics ; viruses ; Young Adult</subject><ispartof>Virology journal, 2013-09, Vol.10 (1), p.283-283, Article 283</ispartof><rights>COPYRIGHT 2013 BioMed Central Ltd.</rights><rights>2013 Hardie et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2013 Hardie et al.; licensee BioMed Central Ltd. 2013 Hardie et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c615t-cf19af3413edc1c9b4e72b511ae730f1f5343f9e447fd541960fd7f7b3337f023</citedby><cites>FETCH-LOGICAL-c615t-cf19af3413edc1c9b4e72b511ae730f1f5343f9e447fd541960fd7f7b3337f023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3847183/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3847183/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24025157$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hardie, Diana R</creatorcontrib><creatorcontrib>Albertyn, Christine</creatorcontrib><creatorcontrib>Heckmann, Jeannine M</creatorcontrib><creatorcontrib>Smuts, Heidi EM</creatorcontrib><title>Molecular characterisation of virus in the brains of patients with measles inclusion body encephalitis (MIBE)</title><title>Virology journal</title><addtitle>Virol J</addtitle><description>BACKGROUND: During 2009/10 a major measles epidemic caused by genotype B3 occurred in South Africa. Measles inclusion body encephalitis (MIBE) was diagnosed in a number of highly immuno-compromised HIV patients. The diagnosis was based on typical clinical and MRI findings and positive measles virus PCR in brain or CSF. To characterize the brain virus, nucleoprotein, matrix, fusion and haemagglutinin genes from 4 cases was compared with virus from acutely infected patients. METHODS: cDNA was synthesized using random primers and viral genes were amplified by nested RT-PCR. PCR products were sequenced in the forward and reverse direction and a contig of each gene was created. Sequences were aligned with reference sequences from GenBank and other local sequences. RESULTS: Brain virus was very similar to the South African epidemic virus. Features characteristic of persistent measles virus in the brain were absent. Mutation frequency in brain virus was similar to epidemic virus and had the same substitution preference (U to C and C to U). The virus of 2 patients had the same L454W mutation in the fusion protein. CONCLUSION: The brain virus was very similar to the epidemic strain. The relatively few mutations probably reflect the short time from infection to brain disease in these highly immuno-compromised patients.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Brain</subject><subject>Brain - virology</subject><subject>Brain research</subject><subject>Children & youth</subject><subject>Cluster Analysis</subject><subject>Colleges & universities</subject><subject>complementary DNA</subject><subject>Complications and side effects</subject><subject>encephalitis</subject><subject>Female</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>genetic databases</subject><subject>genotype</subject><subject>Genotype & phenotype</subject><subject>Health aspects</subject><subject>hemagglutinins</subject><subject>HIV</subject><subject>HIV (Viruses)</subject><subject>HIV patients</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>magnetic resonance imaging</subject><subject>Male</subject><subject>Measles</subject><subject>Measles morbillivirus</subject><subject>Measles virus</subject><subject>Measles virus - genetics</subject><subject>Measles virus - isolation & purification</subject><subject>Molecular Sequence Data</subject><subject>mutation</subject><subject>Mutation Rate</subject><subject>nucleoproteins</subject><subject>Patients</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Physiological aspects</subject><subject>Point Mutation</subject><subject>Polymerase Chain Reaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Viral - genetics</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>South Africa</subject><subject>Subacute sclerosing panencephalitis</subject><subject>Subacute Sclerosing Panencephalitis - virology</subject><subject>Trees</subject><subject>Values</subject><subject>Viral Proteins - genetics</subject><subject>viruses</subject><subject>Young Adult</subject><issn>1743-422X</issn><issn>1743-422X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqNks9rFDEUxwdRbK3ePWnAS3uYmp-TyUWopepCi2AteAuZzMtuysxkTWaq_e_NuHXtiqDkkPDN5315efkWxXOCjwmpq9dEclZySr-UBJe0Zg-K_a308N55r3iS0jXGjFZSPS72KMdUECH3i_4idGCnzkRkVyYaO0L0yYw-DCg4dOPjlJAf0LgC1ETjhzTL6wzAMCb0zY8r1INJHcyY7aY0VzahvUUwWFivTOdHn9DhxeLt2dHT4pEzXYJnd_tBcfXu7PPph_L84_vF6cl5aSsixtI6ooxjnDBoLbGq4SBpIwgxIBl2xAnGmVPAuXSt4ERV2LXSyYYxJh2m7KB4s_FdT02fPXKv0XR6HX1v4q0Oxuvdm8Gv9DLcaFZzSWqWDQ7vDGL4OkEade-Tha4zA4QpaSJzC5RTxf-NcqEIV7US_4Hy_IBa0tn11R_odZjikIeWKVbXSqlK_aaWpgPtBxfya-xsqk9yg5XiVOBMHf-FyquF3tswgPNZ3yk42inIzAjfx6WZUtKLy0-7LN6wNoaUIrjtlAnWc0j1nEI9p3BW6M_hvrj_O9uCX6nMwMsN4EzQZpkDqa8uKSYCY0pqqSj7AQLm6Qs</recordid><startdate>20130912</startdate><enddate>20130912</enddate><creator>Hardie, Diana R</creator><creator>Albertyn, Christine</creator><creator>Heckmann, Jeannine M</creator><creator>Smuts, Heidi EM</creator><general>Springer-Verlag</general><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7T2</scope><scope>7U2</scope><scope>C1K</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20130912</creationdate><title>Molecular characterisation of virus in the brains of patients with measles inclusion body encephalitis (MIBE)</title><author>Hardie, Diana R ; Albertyn, Christine ; Heckmann, Jeannine M ; Smuts, Heidi EM</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c615t-cf19af3413edc1c9b4e72b511ae730f1f5343f9e447fd541960fd7f7b3337f023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Brain</topic><topic>Brain - virology</topic><topic>Brain research</topic><topic>Children & youth</topic><topic>Cluster Analysis</topic><topic>Colleges & universities</topic><topic>complementary DNA</topic><topic>Complications and side effects</topic><topic>encephalitis</topic><topic>Female</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>genetic databases</topic><topic>genotype</topic><topic>Genotype & phenotype</topic><topic>Health aspects</topic><topic>hemagglutinins</topic><topic>HIV</topic><topic>HIV (Viruses)</topic><topic>HIV patients</topic><topic>Human immunodeficiency virus</topic><topic>Humans</topic><topic>magnetic resonance imaging</topic><topic>Male</topic><topic>Measles</topic><topic>Measles morbillivirus</topic><topic>Measles virus</topic><topic>Measles virus - genetics</topic><topic>Measles virus - isolation & purification</topic><topic>Molecular Sequence Data</topic><topic>mutation</topic><topic>Mutation Rate</topic><topic>nucleoproteins</topic><topic>Patients</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Physiological aspects</topic><topic>Point Mutation</topic><topic>Polymerase Chain Reaction</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Viral - genetics</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>South Africa</topic><topic>Subacute sclerosing panencephalitis</topic><topic>Subacute Sclerosing Panencephalitis - virology</topic><topic>Trees</topic><topic>Values</topic><topic>Viral Proteins - genetics</topic><topic>viruses</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hardie, Diana R</creatorcontrib><creatorcontrib>Albertyn, Christine</creatorcontrib><creatorcontrib>Heckmann, Jeannine M</creatorcontrib><creatorcontrib>Smuts, Heidi EM</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Safety Science and Risk</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Virology journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hardie, Diana R</au><au>Albertyn, Christine</au><au>Heckmann, Jeannine M</au><au>Smuts, Heidi EM</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterisation of virus in the brains of patients with measles inclusion body encephalitis (MIBE)</atitle><jtitle>Virology journal</jtitle><addtitle>Virol J</addtitle><date>2013-09-12</date><risdate>2013</risdate><volume>10</volume><issue>1</issue><spage>283</spage><epage>283</epage><pages>283-283</pages><artnum>283</artnum><issn>1743-422X</issn><eissn>1743-422X</eissn><abstract>BACKGROUND: During 2009/10 a major measles epidemic caused by genotype B3 occurred in South Africa. Measles inclusion body encephalitis (MIBE) was diagnosed in a number of highly immuno-compromised HIV patients. The diagnosis was based on typical clinical and MRI findings and positive measles virus PCR in brain or CSF. To characterize the brain virus, nucleoprotein, matrix, fusion and haemagglutinin genes from 4 cases was compared with virus from acutely infected patients. METHODS: cDNA was synthesized using random primers and viral genes were amplified by nested RT-PCR. PCR products were sequenced in the forward and reverse direction and a contig of each gene was created. Sequences were aligned with reference sequences from GenBank and other local sequences. RESULTS: Brain virus was very similar to the South African epidemic virus. Features characteristic of persistent measles virus in the brain were absent. Mutation frequency in brain virus was similar to epidemic virus and had the same substitution preference (U to C and C to U). The virus of 2 patients had the same L454W mutation in the fusion protein. CONCLUSION: The brain virus was very similar to the epidemic strain. The relatively few mutations probably reflect the short time from infection to brain disease in these highly immuno-compromised patients.</abstract><cop>England</cop><pub>Springer-Verlag</pub><pmid>24025157</pmid><doi>10.1186/1743-422X-10-283</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Brain Brain - virology Brain research Children & youth Cluster Analysis Colleges & universities complementary DNA Complications and side effects encephalitis Female Genes Genetic aspects genetic databases genotype Genotype & phenotype Health aspects hemagglutinins HIV HIV (Viruses) HIV patients Human immunodeficiency virus Humans magnetic resonance imaging Male Measles Measles morbillivirus Measles virus Measles virus - genetics Measles virus - isolation & purification Molecular Sequence Data mutation Mutation Rate nucleoproteins Patients Phylogenetics Phylogeny Physiological aspects Point Mutation Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - genetics Sequence Alignment Sequence Analysis, DNA South Africa Subacute sclerosing panencephalitis Subacute Sclerosing Panencephalitis - virology Trees Values Viral Proteins - genetics viruses Young Adult |
| title | Molecular characterisation of virus in the brains of patients with measles inclusion body encephalitis (MIBE) |
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