A Model of Chronic Nutrient Infusion in the Rat
Chronic exposure to excessive levels of nutrients is postulated to affect the function of several organs and tissues and to contribute to the development of the many complications associated with obesity and the metabolic syndrome, including type 2 diabetes. To study the mechanisms by which excessiv...
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creator | Fergusson, Grace Ethier, Mélanie Zarrouki, Bader Fontés, Ghislaine Poitout, Vincent |
description | Chronic exposure to excessive levels of nutrients is postulated to affect the function of several organs and tissues and to contribute to the development of the many complications associated with obesity and the metabolic syndrome, including type 2 diabetes. To study the mechanisms by which excessive levels of glucose and fatty acids affect the pancreatic beta-cell and the secretion of insulin, we have established a chronic nutrient infusion model in the rat. The procedure consists of catheterizing the right jugular vein and left carotid artery under general anesthesia; allowing a 7-day recuperation period; connecting the catheters to the pumps using a swivel and counterweight system that enables the animal to move freely in the cage; and infusing glucose and/or Intralipid (a soybean oil emulsion which generates a mixture of approximately 80% unsaturated/20% saturated fatty acids when infused with heparin) for 72 hr. This model offers several advantages, including the possibility to finely modulate the target levels of circulating glucose and fatty acids; the option to co-infuse pharmacological compounds; and the relatively short time frame as opposed to dietary models. It can be used to examine the mechanisms of nutrient-induced dysfunction in a variety of organs and to test the effectiveness of drugs in this context. |
doi_str_mv | 10.3791/50267 |
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It can be used to examine the mechanisms of nutrient-induced dysfunction in a variety of organs and to test the effectiveness of drugs in this context.</description><subject>Animals</subject><subject>Biomedical Engineering</subject><subject>Blood Glucose - analysis</subject><subject>Blood Glucose - metabolism</subject><subject>Catheterization, Central Venous - methods</subject><subject>Emulsions - administration & dosage</subject><subject>Enteral Nutrition - adverse effects</subject><subject>Enteral Nutrition - methods</subject><subject>Glucose - administration & dosage</subject><subject>Models, Animal</subject><subject>Phospholipids - administration & dosage</subject><subject>Phospholipids - blood</subject><subject>Rats</subject><subject>Soybean Oil - administration & dosage</subject><subject>Soybean Oil - blood</subject><issn>1940-087X</issn><issn>1940-087X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkEtLAzEUhYMottb-AReSjeBmNK_JZDZCKT4KVUEU3IVMktqUaVKTmYL_3tHWUlf3wv3OuYcDwBCjK1qU-DpHhBcHoI9LhjIkivfDvb0HTlJaIMQJysUx6BFadhqc98H1CD4GY2sYZnA8j8E7DZ_aJjrrGzjxsza54KHzsJlb-KKaU3A0U3Wyw-0cgLe729fxQzZ9vp-MR9NMM8GbjFqlaIE0IxU2BRFlwYwuhOHUCCEwKxDBlFeCa4GRpqzEtqysrYywCqvc0AG42fiu2mppje7iRFXLVXRLFb9kUE7-v3g3lx9hLalgvMzLzuByaxDDZ2tTI5cuaVvXytvQJokZETnmRPAOvdigOoaUop3t3mAkf8qVv-V23Pl-ph3112YHnG2ARVhbuQht9F1HW_U3mP17aw</recordid><startdate>20130814</startdate><enddate>20130814</enddate><creator>Fergusson, Grace</creator><creator>Ethier, Mélanie</creator><creator>Zarrouki, Bader</creator><creator>Fontés, Ghislaine</creator><creator>Poitout, Vincent</creator><general>MyJove Corporation</general><scope>BTACS</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130814</creationdate><title>A Model of Chronic Nutrient Infusion in the Rat</title><author>Fergusson, Grace ; Ethier, Mélanie ; Zarrouki, Bader ; Fontés, Ghislaine ; Poitout, Vincent</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-3eaa370c42b1d728974dc78d63d88814702136b86c810c3491e9beebd8ea1a5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Biomedical Engineering</topic><topic>Blood Glucose - analysis</topic><topic>Blood Glucose - metabolism</topic><topic>Catheterization, Central Venous - methods</topic><topic>Emulsions - administration & dosage</topic><topic>Enteral Nutrition - adverse effects</topic><topic>Enteral Nutrition - methods</topic><topic>Glucose - administration & dosage</topic><topic>Models, Animal</topic><topic>Phospholipids - administration & dosage</topic><topic>Phospholipids - blood</topic><topic>Rats</topic><topic>Soybean Oil - administration & dosage</topic><topic>Soybean Oil - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fergusson, Grace</creatorcontrib><creatorcontrib>Ethier, Mélanie</creatorcontrib><creatorcontrib>Zarrouki, Bader</creatorcontrib><creatorcontrib>Fontés, Ghislaine</creatorcontrib><creatorcontrib>Poitout, Vincent</creatorcontrib><collection>JoVE Journal: Medicine</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Visualized Experiments</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Fergusson, Grace</au><au>Ethier, Mélanie</au><au>Zarrouki, Bader</au><au>Fontés, Ghislaine</au><au>Poitout, Vincent</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Model of Chronic Nutrient Infusion in the Rat</atitle><jtitle>Journal of Visualized Experiments</jtitle><addtitle>J Vis Exp</addtitle><date>2013-08-14</date><risdate>2013</risdate><issue>78</issue><issn>1940-087X</issn><eissn>1940-087X</eissn><abstract>Chronic exposure to excessive levels of nutrients is postulated to affect the function of several organs and tissues and to contribute to the development of the many complications associated with obesity and the metabolic syndrome, including type 2 diabetes. To study the mechanisms by which excessive levels of glucose and fatty acids affect the pancreatic beta-cell and the secretion of insulin, we have established a chronic nutrient infusion model in the rat. The procedure consists of catheterizing the right jugular vein and left carotid artery under general anesthesia; allowing a 7-day recuperation period; connecting the catheters to the pumps using a swivel and counterweight system that enables the animal to move freely in the cage; and infusing glucose and/or Intralipid (a soybean oil emulsion which generates a mixture of approximately 80% unsaturated/20% saturated fatty acids when infused with heparin) for 72 hr. This model offers several advantages, including the possibility to finely modulate the target levels of circulating glucose and fatty acids; the option to co-infuse pharmacological compounds; and the relatively short time frame as opposed to dietary models. It can be used to examine the mechanisms of nutrient-induced dysfunction in a variety of organs and to test the effectiveness of drugs in this context.</abstract><cop>United States</cop><pub>MyJove Corporation</pub><pmid>23979115</pmid><doi>10.3791/50267</doi><oa>free_for_read</oa></addata></record> |
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subjects | Animals Biomedical Engineering Blood Glucose - analysis Blood Glucose - metabolism Catheterization, Central Venous - methods Emulsions - administration & dosage Enteral Nutrition - adverse effects Enteral Nutrition - methods Glucose - administration & dosage Models, Animal Phospholipids - administration & dosage Phospholipids - blood Rats Soybean Oil - administration & dosage Soybean Oil - blood |
title | A Model of Chronic Nutrient Infusion in the Rat |
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