High Yields of Oligodendrocyte Lineage Cells from Human Embryonic Stem Cells at Physiological Oxygen Tensions for Evaluation of Translational Biology

We have established and efficient system to specify NG2/PDGF-Rα/OLIG2+ oligodendrocyte precursor cells (OPCs) from human embryonic stem cells (hESCs) at low, physiological (3%) oxygen levels. This was achieved via both forebrain and spinal cord origins, with up to 98% of cells expressing NG2. Develo...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Stem cell reports 2013-11, Vol.1 (5), p.437-450
Hauptverfasser:	Stacpoole, Sybil R.L., Spitzer, Sonia, Bilican, Bilada, Compston, Alastair, Karadottir, Ragnhildur, Chandran, Siddharthan, Franklin, Robin J.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Action Potentials Basic Helix-Loop-Helix Transcription Factors - genetics Basic Helix-Loop-Helix Transcription Factors - metabolism Cell Lineage Cells, Cultured Embryonic Stem Cells - cytology Embryonic Stem Cells - drug effects Embryonic Stem Cells - metabolism Fibroblast Growth Factor 2 - pharmacology Galactosylceramides - metabolism Humans Myelin Basic Protein - genetics Myelin Basic Protein - metabolism Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Neural Stem Cells - cytology Neural Stem Cells - drug effects Neural Stem Cells - metabolism Neural Stem Cells - physiology Neurogenesis Oligodendrocyte Transcription Factor 2 Oligodendroglia - cytology Oligodendroglia - drug effects Oligodendroglia - metabolism Oligodendroglia - physiology Oxygen - pharmacology Prosencephalon - cytology Retinoid X Receptors - antagonists & inhibitors Sodium - metabolism Spinal Cord - cytology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!