Pig transgenesis by piggyBac transposition in combination with somatic cell nuclear transfer

The production of animals by somatic cell nuclear transfer (SCNT) is inefficient, with approximately 2 % of micromanipulated oocytes going to term and resulting in live births. However, it is the most commonly used method for the generation of cloned transgenic livestock as it facilitates the attain...

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Veröffentlicht in:	Transgenic research 2013-12, Vol.22 (6), p.1107-1118
Hauptverfasser:	Wu, Zhenfang, Xu, Zhiqian, Zou, Xian, Zeng, Fang, Shi, Junsong, Liu, Dewu, Urschitz, Johann, Moisyadi, Stefan, Li, Zicong
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Genetics and Genomics Animals Animals, Genetically Modified - genetics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biotechnology DNA Transposable Elements - genetics farms Fibroblasts - cytology Fundamental and applied biological sciences. Psychology Gene Transfer Techniques Genetic Engineering Genetic technics genetically modified organisms Green Fluorescent Proteins - genetics Life Sciences Methods. Procedures. Technologies Molecular Medicine Nuclear Transfer Techniques oocytes Oocytes - cytology Oocytes - growth & development Original Paper Plant Genetics and Genomics somatic cells swine Swine - genetics transfection Transgenes transgenesis transgenic animals Transgenic animals and transgenic plants Transgenics transposition (genetics)
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