Radical SAM-Dependent Carbon Insertion into the Nitrogenase M-Cluster

The active site of nitrogenase, the M-cluster, is a metal-sulfur cluster containing a carbide at its core. Using radiolabeling experiments, we show that this carbide originates from the methyl group of S-adenosylmethionine (SAM) and that it is inserted into the M-cluster by the assembly protein NifB...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2012-09, Vol.337 (6102), p.1672-1675
Hauptverfasser:	Wiig, Jared A., Hu, Yilin, Lee, Chi Chung, Ribbe, Markus W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aluminum ammonia Analytical, structural and metabolic biochemistry Atoms Bacteria Bacterial Proteins - chemistry Biological and medical sciences Carbides Carbon Carbon - chemistry Catalysis Catalytic Domain Deuterium Deuterium Exchange Measurement Enzymes Fundamental and applied biological sciences. Psychology Hydrogen Insertion Ligands Metal clusters Methylation Methyltransferases - chemistry Molecular and cellular biology Nitrogen nitrogen cycle nitrogenase Nitrogenase - chemistry Radicals Radioactive decay Radiocarbon Resins Resultants RNA - chemistry S-adenosylmethionine S-Adenosylmethionine - chemistry
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