SDF-1α in Glycan Nanoparticles Exhibits Full Activity and Reduces Pulmonary Hypertension in Rats
To establish a homing signal in the lung to recruit circulating stem cells for tissue repair, we formulated a nanoparticle, SDF-1α NP, by complexing SDF-1α with dextran sulfate and chitosan. The data show that SDF-1α was barely released from the nanoparticles over an extended period of time in vitro...
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| Veröffentlicht in: | Biomacromolecules 2013-11, Vol.14 (11), p.4009-4020 |
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| creator | Yin, Tao Bader, Andrew R Hou, Tim K Maron, Bradley A Kao, Derrick D Qian, Ray Kohane, Daniel S Handy, Diane E Loscalzo, Joseph Zhang, Ying-Yi |
| description | To establish a homing signal in the lung to recruit circulating stem cells for tissue repair, we formulated a nanoparticle, SDF-1α NP, by complexing SDF-1α with dextran sulfate and chitosan. The data show that SDF-1α was barely released from the nanoparticles over an extended period of time in vitro (3% in 7 days at 37 °C); however, incorporated SDF-1α exhibited full chemotactic activity and receptor activation compared to its free form. The nanoparticles were not endocytosed after incubation with Jurkat cells. When aerosolized into the lungs of rats, SDF-1α NP displayed a greater retention time compared to free SDF-1α (64 vs 2% remaining at 16 h). In a rat model of monocrotaline-induced lung injury, SDF-1α NP, but not free form SDF-1α, was found to reduce pulmonary hypertension. These data suggest that the nanoparticle formulation protected SDF-1α from rapid clearance in the lung and sustained its biological function in vivo. |
| doi_str_mv | 10.1021/bm401122q |
| format | Article |
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The data show that SDF-1α was barely released from the nanoparticles over an extended period of time in vitro (3% in 7 days at 37 °C); however, incorporated SDF-1α exhibited full chemotactic activity and receptor activation compared to its free form. The nanoparticles were not endocytosed after incubation with Jurkat cells. When aerosolized into the lungs of rats, SDF-1α NP displayed a greater retention time compared to free SDF-1α (64 vs 2% remaining at 16 h). In a rat model of monocrotaline-induced lung injury, SDF-1α NP, but not free form SDF-1α, was found to reduce pulmonary hypertension. These data suggest that the nanoparticle formulation protected SDF-1α from rapid clearance in the lung and sustained its biological function in vivo.</description><identifier>ISSN: 1525-7797</identifier><identifier>EISSN: 1526-4602</identifier><identifier>DOI: 10.1021/bm401122q</identifier><identifier>PMID: 24059347</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Aerosols ; Animals ; Antihypertensive agents ; Applied sciences ; Biological and medical sciences ; Cardiovascular system ; Chemokine CXCL12 - administration & dosage ; Chemokine CXCL12 - pharmacokinetics ; Chemokine CXCL12 - pharmacology ; Chemokine CXCL12 - therapeutic use ; Chitosan - chemistry ; Dextran Sulfate - chemistry ; Exact sciences and technology ; Forms of application and semi-finished materials ; Humans ; Hypertension, Pulmonary - chemically induced ; Hypertension, Pulmonary - drug therapy ; Hypertension, Pulmonary - prevention & control ; Jurkat Cells ; Male ; Medical sciences ; Miscellaneous ; Monocrotaline ; Nanoparticles - administration & dosage ; Nanoparticles - chemistry ; Pharmacology. 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These data suggest that the nanoparticle formulation protected SDF-1α from rapid clearance in the lung and sustained its biological function in vivo.</description><subject>Aerosols</subject><subject>Animals</subject><subject>Antihypertensive agents</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Cardiovascular system</subject><subject>Chemokine CXCL12 - administration & dosage</subject><subject>Chemokine CXCL12 - pharmacokinetics</subject><subject>Chemokine CXCL12 - pharmacology</subject><subject>Chemokine CXCL12 - therapeutic use</subject><subject>Chitosan - chemistry</subject><subject>Dextran Sulfate - chemistry</subject><subject>Exact sciences and technology</subject><subject>Forms of application and semi-finished materials</subject><subject>Humans</subject><subject>Hypertension, Pulmonary - chemically induced</subject><subject>Hypertension, Pulmonary - drug therapy</subject><subject>Hypertension, Pulmonary - prevention & control</subject><subject>Jurkat Cells</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Miscellaneous</subject><subject>Monocrotaline</subject><subject>Nanoparticles - administration & dosage</subject><subject>Nanoparticles - chemistry</subject><subject>Pharmacology. Drug treatments</subject><subject>Polymer industry, paints, wood</subject><subject>Polysaccharides - administration & dosage</subject><subject>Polysaccharides - chemistry</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Technology of polymers</subject><subject>Time Factors</subject><issn>1525-7797</issn><issn>1526-4602</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkd1qFTEUhYMotlYvfAHJjVAvRvM7ydwIpfa0QlGpeh0yScamZJLTJFM8j-WL-Eym9niq4NXesD_WWuwFwHOMXmNE8JtxZghjQq4fgH3MSd-xHpGHv3feCTGIPfCklCuE0EAZfwz2CEO8rWIf6M_vVh3--QP6CE_DxugIP-iY1jpXb4Ir8OT7pR99LXC1hACPTPU3vm6gjhZeOLuYhnxawpyizht4tlm7XF0sPsVbxQtdy1PwaNKhuGfbeQC-rk6-HJ915x9P3x8fnXea9bJ2tueSWcwtQ0gyN1khmRy4RVb0RmI59CPqNZnsODgqHNZcCkYnR4hgvCeWHoC3d7rrZZydNS7WrINaZz-3aCppr_69RH-pvqUbRSWljOEmcLgVyOl6caWq2RfjQtDRpaWo9k3GCKVENvTVHWpyKiW7aWeDkbqtRO0qaeyLv3PtyD8dNODlFtDF6DBlHY0v95wYmORkuOe0KeoqLTm2d_7H8Be4tqCA</recordid><startdate>20131111</startdate><enddate>20131111</enddate><creator>Yin, Tao</creator><creator>Bader, Andrew R</creator><creator>Hou, Tim K</creator><creator>Maron, Bradley A</creator><creator>Kao, Derrick D</creator><creator>Qian, Ray</creator><creator>Kohane, Daniel S</creator><creator>Handy, Diane E</creator><creator>Loscalzo, Joseph</creator><creator>Zhang, Ying-Yi</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20131111</creationdate><title>SDF-1α in Glycan Nanoparticles Exhibits Full Activity and Reduces Pulmonary Hypertension in Rats</title><author>Yin, Tao ; Bader, Andrew R ; Hou, Tim K ; Maron, Bradley A ; Kao, Derrick D ; Qian, Ray ; Kohane, Daniel S ; Handy, Diane E ; Loscalzo, Joseph ; Zhang, Ying-Yi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a468t-d6584d15d40084efd784895d0d76c81896b06a2fdb9e37e1a58743fe2274562d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Aerosols</topic><topic>Animals</topic><topic>Antihypertensive agents</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Cardiovascular system</topic><topic>Chemokine CXCL12 - administration & dosage</topic><topic>Chemokine CXCL12 - pharmacokinetics</topic><topic>Chemokine CXCL12 - pharmacology</topic><topic>Chemokine CXCL12 - therapeutic use</topic><topic>Chitosan - chemistry</topic><topic>Dextran Sulfate - chemistry</topic><topic>Exact sciences and technology</topic><topic>Forms of application and semi-finished materials</topic><topic>Humans</topic><topic>Hypertension, Pulmonary - chemically induced</topic><topic>Hypertension, Pulmonary - drug therapy</topic><topic>Hypertension, Pulmonary - prevention & control</topic><topic>Jurkat Cells</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Miscellaneous</topic><topic>Monocrotaline</topic><topic>Nanoparticles - administration & dosage</topic><topic>Nanoparticles - chemistry</topic><topic>Pharmacology. Drug treatments</topic><topic>Polymer industry, paints, wood</topic><topic>Polysaccharides - administration & dosage</topic><topic>Polysaccharides - chemistry</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Technology of polymers</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yin, Tao</creatorcontrib><creatorcontrib>Bader, Andrew R</creatorcontrib><creatorcontrib>Hou, Tim K</creatorcontrib><creatorcontrib>Maron, Bradley A</creatorcontrib><creatorcontrib>Kao, Derrick D</creatorcontrib><creatorcontrib>Qian, Ray</creatorcontrib><creatorcontrib>Kohane, Daniel S</creatorcontrib><creatorcontrib>Handy, Diane E</creatorcontrib><creatorcontrib>Loscalzo, Joseph</creatorcontrib><creatorcontrib>Zhang, Ying-Yi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biomacromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yin, Tao</au><au>Bader, Andrew R</au><au>Hou, Tim K</au><au>Maron, Bradley A</au><au>Kao, Derrick D</au><au>Qian, Ray</au><au>Kohane, Daniel S</au><au>Handy, Diane E</au><au>Loscalzo, Joseph</au><au>Zhang, Ying-Yi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SDF-1α in Glycan Nanoparticles Exhibits Full Activity and Reduces Pulmonary Hypertension in Rats</atitle><jtitle>Biomacromolecules</jtitle><addtitle>Biomacromolecules</addtitle><date>2013-11-11</date><risdate>2013</risdate><volume>14</volume><issue>11</issue><spage>4009</spage><epage>4020</epage><pages>4009-4020</pages><issn>1525-7797</issn><eissn>1526-4602</eissn><abstract>To establish a homing signal in the lung to recruit circulating stem cells for tissue repair, we formulated a nanoparticle, SDF-1α NP, by complexing SDF-1α with dextran sulfate and chitosan. The data show that SDF-1α was barely released from the nanoparticles over an extended period of time in vitro (3% in 7 days at 37 °C); however, incorporated SDF-1α exhibited full chemotactic activity and receptor activation compared to its free form. The nanoparticles were not endocytosed after incubation with Jurkat cells. When aerosolized into the lungs of rats, SDF-1α NP displayed a greater retention time compared to free SDF-1α (64 vs 2% remaining at 16 h). In a rat model of monocrotaline-induced lung injury, SDF-1α NP, but not free form SDF-1α, was found to reduce pulmonary hypertension. These data suggest that the nanoparticle formulation protected SDF-1α from rapid clearance in the lung and sustained its biological function in vivo.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>24059347</pmid><doi>10.1021/bm401122q</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biomacromolecules, 2013-11, Vol.14 (11), p.4009-4020 |
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| subjects | Aerosols Animals Antihypertensive agents Applied sciences Biological and medical sciences Cardiovascular system Chemokine CXCL12 - administration & dosage Chemokine CXCL12 - pharmacokinetics Chemokine CXCL12 - pharmacology Chemokine CXCL12 - therapeutic use Chitosan - chemistry Dextran Sulfate - chemistry Exact sciences and technology Forms of application and semi-finished materials Humans Hypertension, Pulmonary - chemically induced Hypertension, Pulmonary - drug therapy Hypertension, Pulmonary - prevention & control Jurkat Cells Male Medical sciences Miscellaneous Monocrotaline Nanoparticles - administration & dosage Nanoparticles - chemistry Pharmacology. Drug treatments Polymer industry, paints, wood Polysaccharides - administration & dosage Polysaccharides - chemistry Rats Rats, Sprague-Dawley Technology of polymers Time Factors |
| title | SDF-1α in Glycan Nanoparticles Exhibits Full Activity and Reduces Pulmonary Hypertension in Rats |
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