Comparative proteomic and transcriptomic approaches to address the active role of GA4 in Japanese apricot flower bud dormancy release
Hormones are closely associated with dormancy in deciduous fruit trees, and gibberellins (GAs) are known to be particularly important. In this study, we observed that GA4 treatment led to earlier bud break in Japanese apricot. To understand better the promoting effect of GA4 on the dormancy release...
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| Veröffentlicht in: | Journal of experimental botany 2013-11, Vol.64 (16), p.4953-4966 |
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| creator | Zhuang, Weibing Gao, Zhihong Wang, Liangju Zhong, Wenjun Ni, Zhaojun Zhang, Zhen |
| description | Hormones are closely associated with dormancy in deciduous fruit trees, and gibberellins (GAs) are known to be particularly important. In this study, we observed that GA4 treatment led to earlier bud break in Japanese apricot. To understand better the promoting effect of GA4 on the dormancy release of Japanese apricot flower buds, proteomic and transcriptomic approaches were used to analyse the mechanisms of dormancy release following GA4 treatment, based on two-dimensional gel electrophoresis (2-DE) and digital gene expression (DGE) profiling, respectively. More than 600 highly reproducible protein spots (P |
| doi_str_mv | 10.1093/jxb/ert284 |
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In this study, we observed that GA4 treatment led to earlier bud break in Japanese apricot. To understand better the promoting effect of GA4 on the dormancy release of Japanese apricot flower buds, proteomic and transcriptomic approaches were used to analyse the mechanisms of dormancy release following GA4 treatment, based on two-dimensional gel electrophoresis (2-DE) and digital gene expression (DGE) profiling, respectively. More than 600 highly reproducible protein spots (P<0.05) were detected and, following GA4 treatment, 38 protein spots showed more than a 2-fold difference in expression, and 32 protein spots were confidently identified according to the databases. Compared with water treatment, many proteins that were associated with energy metabolism and oxidation–reduction showed significant changes after GA4 treatment, which might promote dormancy release. We observed that genes at the mRNA level associated with energy metabolism and oxidation–reduction also played an important role in this process. Analysis of the functions of the identified proteins and genes and the related metabolic pathways would provide a comprehensive proteomic and transcriptomic view of the coordination of dormancy release after GA4 treatment in Japanese apricot flower buds.</description><identifier>ISSN: 0022-0957</identifier><identifier>EISSN: 1460-2431</identifier><identifier>DOI: 10.1093/jxb/ert284</identifier><identifier>PMID: 24014872</identifier><identifier>CODEN: JEBOA6</identifier><language>eng</language><publisher>Oxford: Oxford University Press [etc.]</publisher><subject>biochemical pathways ; Biological and medical sciences ; budbreak ; buds ; chemistry ; dormancy ; Electrophoresis, Gel, Two-Dimensional ; Electrophoresis, Gel, Two-Dimensional - methods ; energy metabolism ; Flowers ; Flowers - chemistry ; Flowers - genetics ; Flowers - growth & development ; Flowers - metabolism ; fruit trees ; Fundamental and applied biological sciences. Psychology ; gene expression ; Gene Expression Profiling ; Gene Expression Profiling - methods ; genes ; genetics ; Germination and dormancy ; gibberellins ; Gibberellins - metabolism ; growth & development ; hormones ; messenger RNA ; metabolism ; methods ; Plant physiology and development ; Plant Proteins ; Plant Proteins - chemistry ; Plant Proteins - genetics ; Plant Proteins - metabolism ; proteins ; proteomics ; Proteomics - methods ; Prunus ; Prunus - chemistry ; Prunus - genetics ; Prunus - growth & development ; Prunus - metabolism ; Prunus mume ; Research Paper ; transcriptomics ; two-dimensional gel electrophoresis ; water treatment</subject><ispartof>Journal of experimental botany, 2013-11, Vol.64 (16), p.4953-4966</ispartof><rights>2015 INIST-CNRS</rights><rights>The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4464-a4f1cf6f82f3bf2def6760767b639497bb89ec956d4256a55b811324e7d6834d3</citedby><cites>FETCH-LOGICAL-c4464-a4f1cf6f82f3bf2def6760767b639497bb89ec956d4256a55b811324e7d6834d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27915,27916</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27959908$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24014872$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhuang, Weibing</creatorcontrib><creatorcontrib>Gao, Zhihong</creatorcontrib><creatorcontrib>Wang, Liangju</creatorcontrib><creatorcontrib>Zhong, Wenjun</creatorcontrib><creatorcontrib>Ni, Zhaojun</creatorcontrib><creatorcontrib>Zhang, Zhen</creatorcontrib><title>Comparative proteomic and transcriptomic approaches to address the active role of GA4 in Japanese apricot flower bud dormancy release</title><title>Journal of experimental botany</title><addtitle>J Exp Bot</addtitle><description>Hormones are closely associated with dormancy in deciduous fruit trees, and gibberellins (GAs) are known to be particularly important. In this study, we observed that GA4 treatment led to earlier bud break in Japanese apricot. To understand better the promoting effect of GA4 on the dormancy release of Japanese apricot flower buds, proteomic and transcriptomic approaches were used to analyse the mechanisms of dormancy release following GA4 treatment, based on two-dimensional gel electrophoresis (2-DE) and digital gene expression (DGE) profiling, respectively. More than 600 highly reproducible protein spots (P<0.05) were detected and, following GA4 treatment, 38 protein spots showed more than a 2-fold difference in expression, and 32 protein spots were confidently identified according to the databases. Compared with water treatment, many proteins that were associated with energy metabolism and oxidation–reduction showed significant changes after GA4 treatment, which might promote dormancy release. We observed that genes at the mRNA level associated with energy metabolism and oxidation–reduction also played an important role in this process. Analysis of the functions of the identified proteins and genes and the related metabolic pathways would provide a comprehensive proteomic and transcriptomic view of the coordination of dormancy release after GA4 treatment in Japanese apricot flower buds.</description><subject>biochemical pathways</subject><subject>Biological and medical sciences</subject><subject>budbreak</subject><subject>buds</subject><subject>chemistry</subject><subject>dormancy</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Electrophoresis, Gel, Two-Dimensional - methods</subject><subject>energy metabolism</subject><subject>Flowers</subject><subject>Flowers - chemistry</subject><subject>Flowers - genetics</subject><subject>Flowers - growth & development</subject><subject>Flowers - metabolism</subject><subject>fruit trees</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Profiling - methods</subject><subject>genes</subject><subject>genetics</subject><subject>Germination and dormancy</subject><subject>gibberellins</subject><subject>Gibberellins - metabolism</subject><subject>growth & development</subject><subject>hormones</subject><subject>messenger RNA</subject><subject>metabolism</subject><subject>methods</subject><subject>Plant physiology and development</subject><subject>Plant Proteins</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>proteins</subject><subject>proteomics</subject><subject>Proteomics - methods</subject><subject>Prunus</subject><subject>Prunus - chemistry</subject><subject>Prunus - genetics</subject><subject>Prunus - growth & development</subject><subject>Prunus - metabolism</subject><subject>Prunus mume</subject><subject>Research Paper</subject><subject>transcriptomics</subject><subject>two-dimensional gel electrophoresis</subject><subject>water treatment</subject><issn>0022-0957</issn><issn>1460-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1u1DAUhS0EokNhwwOAN0gIKdT_djaVqhEUUCUW0LXl2NedVEkc7EyhD8B7Y8hQYMXKls_no3N1LkJPKXlNSctPrr91J5AXZsQ9tKFCkYYJTu-jDSGMNaSV-gg9KuWaECKJlA_REROECqPZBn3fpnF22S39DeA5pwXS2HvspoCX7Kbicz8v69NcZed3UPCSsAshQ6nXHWDnf_3OaQCcIj4_E7if8Ac3uwlKlefc-7TgOKSvkHG3DzikPLrJ3-IMA7gCj9GD6IYCTw7nMbp8--bz9l1z8fH8_fbsovFCKNE4EamPKhoWeRdZgKi0IlrpTvFWtLrrTAu-lSoIJpWTsjOUciZAB2W4CPwYna6-874bIXiY6pCDrQFHl29tcr39V5n6nb1KN5YbToQh1eDlwSCnL3soix374mEY6qhpXyxViivCmdT_R4WsSQU1vKKvVtTnVEqGeJeIEvuzYlsrtmvFFX729wx36O9OK_DiALji3RBrjb4vfzjdyrYlpnLPVy66ZN1VrszlJ0aorHtCNTWM_wBKgLtf</recordid><startdate>20131101</startdate><enddate>20131101</enddate><creator>Zhuang, Weibing</creator><creator>Gao, Zhihong</creator><creator>Wang, Liangju</creator><creator>Zhong, Wenjun</creator><creator>Ni, Zhaojun</creator><creator>Zhang, Zhen</creator><general>Oxford University Press [etc.]</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20131101</creationdate><title>Comparative proteomic and transcriptomic approaches to address the active role of GA4 in Japanese apricot flower bud dormancy release</title><author>Zhuang, Weibing ; Gao, Zhihong ; Wang, Liangju ; Zhong, Wenjun ; Ni, Zhaojun ; Zhang, Zhen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4464-a4f1cf6f82f3bf2def6760767b639497bb89ec956d4256a55b811324e7d6834d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>biochemical pathways</topic><topic>Biological and medical sciences</topic><topic>budbreak</topic><topic>buds</topic><topic>chemistry</topic><topic>dormancy</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Electrophoresis, Gel, Two-Dimensional - methods</topic><topic>energy metabolism</topic><topic>Flowers</topic><topic>Flowers - chemistry</topic><topic>Flowers - genetics</topic><topic>Flowers - growth & development</topic><topic>Flowers - metabolism</topic><topic>fruit trees</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Profiling - methods</topic><topic>genes</topic><topic>genetics</topic><topic>Germination and dormancy</topic><topic>gibberellins</topic><topic>Gibberellins - metabolism</topic><topic>growth & development</topic><topic>hormones</topic><topic>messenger RNA</topic><topic>metabolism</topic><topic>methods</topic><topic>Plant physiology and development</topic><topic>Plant Proteins</topic><topic>Plant Proteins - chemistry</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>proteins</topic><topic>proteomics</topic><topic>Proteomics - methods</topic><topic>Prunus</topic><topic>Prunus - chemistry</topic><topic>Prunus - genetics</topic><topic>Prunus - growth & development</topic><topic>Prunus - metabolism</topic><topic>Prunus mume</topic><topic>Research Paper</topic><topic>transcriptomics</topic><topic>two-dimensional gel electrophoresis</topic><topic>water treatment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhuang, Weibing</creatorcontrib><creatorcontrib>Gao, Zhihong</creatorcontrib><creatorcontrib>Wang, Liangju</creatorcontrib><creatorcontrib>Zhong, Wenjun</creatorcontrib><creatorcontrib>Ni, Zhaojun</creatorcontrib><creatorcontrib>Zhang, Zhen</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of experimental botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhuang, Weibing</au><au>Gao, Zhihong</au><au>Wang, Liangju</au><au>Zhong, Wenjun</au><au>Ni, Zhaojun</au><au>Zhang, Zhen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative proteomic and transcriptomic approaches to address the active role of GA4 in Japanese apricot flower bud dormancy release</atitle><jtitle>Journal of experimental botany</jtitle><addtitle>J Exp Bot</addtitle><date>2013-11-01</date><risdate>2013</risdate><volume>64</volume><issue>16</issue><spage>4953</spage><epage>4966</epage><pages>4953-4966</pages><issn>0022-0957</issn><eissn>1460-2431</eissn><coden>JEBOA6</coden><abstract>Hormones are closely associated with dormancy in deciduous fruit trees, and gibberellins (GAs) are known to be particularly important. In this study, we observed that GA4 treatment led to earlier bud break in Japanese apricot. To understand better the promoting effect of GA4 on the dormancy release of Japanese apricot flower buds, proteomic and transcriptomic approaches were used to analyse the mechanisms of dormancy release following GA4 treatment, based on two-dimensional gel electrophoresis (2-DE) and digital gene expression (DGE) profiling, respectively. More than 600 highly reproducible protein spots (P<0.05) were detected and, following GA4 treatment, 38 protein spots showed more than a 2-fold difference in expression, and 32 protein spots were confidently identified according to the databases. Compared with water treatment, many proteins that were associated with energy metabolism and oxidation–reduction showed significant changes after GA4 treatment, which might promote dormancy release. We observed that genes at the mRNA level associated with energy metabolism and oxidation–reduction also played an important role in this process. Analysis of the functions of the identified proteins and genes and the related metabolic pathways would provide a comprehensive proteomic and transcriptomic view of the coordination of dormancy release after GA4 treatment in Japanese apricot flower buds.</abstract><cop>Oxford</cop><pub>Oxford University Press [etc.]</pub><pmid>24014872</pmid><doi>10.1093/jxb/ert284</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | biochemical pathways Biological and medical sciences budbreak buds chemistry dormancy Electrophoresis, Gel, Two-Dimensional Electrophoresis, Gel, Two-Dimensional - methods energy metabolism Flowers Flowers - chemistry Flowers - genetics Flowers - growth & development Flowers - metabolism fruit trees Fundamental and applied biological sciences. Psychology gene expression Gene Expression Profiling Gene Expression Profiling - methods genes genetics Germination and dormancy gibberellins Gibberellins - metabolism growth & development hormones messenger RNA metabolism methods Plant physiology and development Plant Proteins Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism proteins proteomics Proteomics - methods Prunus Prunus - chemistry Prunus - genetics Prunus - growth & development Prunus - metabolism Prunus mume Research Paper transcriptomics two-dimensional gel electrophoresis water treatment |
| title | Comparative proteomic and transcriptomic approaches to address the active role of GA4 in Japanese apricot flower bud dormancy release |
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