Establishment of a Novel Lingual Organoid Culture System: Generation of Organoids Having Mature Keratinized Epithelium from Adult Epithelial Stem Cells
Despite the strong need for the establishment of a lingual epithelial cell culture system, a simple and convenient culture method has not yet been established. Here, we report the establishment of a novel lingual epithelium organoid culture system using a three-dimensional matrix and growth factors....
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| Veröffentlicht in: | Scientific reports 2013-11, Vol.3 (1), p.3224, Article 3224 |
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| creator | Hisha, Hiroko Tanaka, Toshihiro Kanno, Shohei Tokuyama, Yoko Komai, Yoshihiro Ohe, Shuichi Yanai, Hirotsugu Omachi, Taichi Ueno, Hiroo |
| description | Despite the strong need for the establishment of a lingual epithelial cell culture system, a simple and convenient culture method has not yet been established. Here, we report the establishment of a novel lingual epithelium organoid culture system using a three-dimensional matrix and growth factors. Histological analyses showed that the generated organoids had both a stratified squamous epithelial cell layer and a stratum corneum. Very recently, we showed via a multicolor lineage tracing method that Bmi1-positive stem cells exist at the base of the epithelial basal layer in the interpapillary pit. Using our new culture system, we found that organoids could be generated by single Bmi1-positive stem cells and that in the established organoids, multiple Bmi1-positive stem cells were generated at the outermost layer. Moreover, we observed that organoids harvested at an early point in culture could be engrafted and maturate in the tongue of recipient mice and that the organoids generated from carcinogen-treated mice had an abnormal morphology. Thus, this culture system presents valuable settings for studying not only the regulatory mechanisms of lingual epithelium but also lingual regeneration and carcinogenesis. |
| doi_str_mv | 10.1038/srep03224 |
| format | Article |
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Here, we report the establishment of a novel lingual epithelium organoid culture system using a three-dimensional matrix and growth factors. Histological analyses showed that the generated organoids had both a stratified squamous epithelial cell layer and a stratum corneum. Very recently, we showed via a multicolor lineage tracing method that Bmi1-positive stem cells exist at the base of the epithelial basal layer in the interpapillary pit. Using our new culture system, we found that organoids could be generated by single Bmi1-positive stem cells and that in the established organoids, multiple Bmi1-positive stem cells were generated at the outermost layer. Moreover, we observed that organoids harvested at an early point in culture could be engrafted and maturate in the tongue of recipient mice and that the organoids generated from carcinogen-treated mice had an abnormal morphology. Thus, this culture system presents valuable settings for studying not only the regulatory mechanisms of lingual epithelium but also lingual regeneration and carcinogenesis.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep03224</identifier><identifier>PMID: 24232854</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>14 ; 14/28 ; 14/35 ; 14/63 ; 631/532 ; 631/80 ; Adult Stem Cells - cytology ; Adult Stem Cells - metabolism ; Adult Stem Cells - physiology ; Animals ; Carcinogenesis ; Carcinogenesis - metabolism ; Carcinogenesis - pathology ; Cell culture ; Cell Culture Techniques - methods ; Epithelial Cells - cytology ; Epithelial Cells - metabolism ; Epithelial Cells - physiology ; Epithelium ; Epithelium - metabolism ; Epithelium - physiology ; Growth factors ; Humanities and Social Sciences ; Lingual epithelium ; Mice ; Mice, Inbred C57BL ; multidisciplinary ; Organoids ; Organoids - cytology ; Organoids - metabolism ; Organoids - physiology ; Polycomb Repressive Complex 1 - metabolism ; Proto-Oncogene Proteins - metabolism ; Regeneration - physiology ; Rodents ; Science ; Stem cell transplantation ; Stem cells ; Stratum corneum ; Tongue ; Tongue - cytology ; Tongue - metabolism ; Tongue - physiology</subject><ispartof>Scientific reports, 2013-11, Vol.3 (1), p.3224, Article 3224</ispartof><rights>The Author(s) 2013</rights><rights>Copyright Nature Publishing Group Nov 2013</rights><rights>Copyright © 2013, Macmillan Publishers Limited. All rights reserved 2013 Macmillan Publishers Limited. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-c9decd94b19055da13e84765b9b369d314cc416e4d885181b5cfc77741fc99023</citedby><cites>FETCH-LOGICAL-c504t-c9decd94b19055da13e84765b9b369d314cc416e4d885181b5cfc77741fc99023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828633/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828633/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24232854$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hisha, Hiroko</creatorcontrib><creatorcontrib>Tanaka, Toshihiro</creatorcontrib><creatorcontrib>Kanno, Shohei</creatorcontrib><creatorcontrib>Tokuyama, Yoko</creatorcontrib><creatorcontrib>Komai, Yoshihiro</creatorcontrib><creatorcontrib>Ohe, Shuichi</creatorcontrib><creatorcontrib>Yanai, Hirotsugu</creatorcontrib><creatorcontrib>Omachi, Taichi</creatorcontrib><creatorcontrib>Ueno, Hiroo</creatorcontrib><title>Establishment of a Novel Lingual Organoid Culture System: Generation of Organoids Having Mature Keratinized Epithelium from Adult Epithelial Stem Cells</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Despite the strong need for the establishment of a lingual epithelial cell culture system, a simple and convenient culture method has not yet been established. 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Thus, this culture system presents valuable settings for studying not only the regulatory mechanisms of lingual epithelium but also lingual regeneration and carcinogenesis.</description><subject>14</subject><subject>14/28</subject><subject>14/35</subject><subject>14/63</subject><subject>631/532</subject><subject>631/80</subject><subject>Adult Stem Cells - cytology</subject><subject>Adult Stem Cells - metabolism</subject><subject>Adult Stem Cells - physiology</subject><subject>Animals</subject><subject>Carcinogenesis</subject><subject>Carcinogenesis - metabolism</subject><subject>Carcinogenesis - pathology</subject><subject>Cell culture</subject><subject>Cell Culture Techniques - methods</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelial Cells - physiology</subject><subject>Epithelium</subject><subject>Epithelium - metabolism</subject><subject>Epithelium - physiology</subject><subject>Growth factors</subject><subject>Humanities and Social Sciences</subject><subject>Lingual epithelium</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>multidisciplinary</subject><subject>Organoids</subject><subject>Organoids - 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cytology</topic><topic>Tongue - metabolism</topic><topic>Tongue - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hisha, Hiroko</creatorcontrib><creatorcontrib>Tanaka, Toshihiro</creatorcontrib><creatorcontrib>Kanno, Shohei</creatorcontrib><creatorcontrib>Tokuyama, Yoko</creatorcontrib><creatorcontrib>Komai, Yoshihiro</creatorcontrib><creatorcontrib>Ohe, Shuichi</creatorcontrib><creatorcontrib>Yanai, Hirotsugu</creatorcontrib><creatorcontrib>Omachi, Taichi</creatorcontrib><creatorcontrib>Ueno, Hiroo</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hisha, Hiroko</au><au>Tanaka, Toshihiro</au><au>Kanno, Shohei</au><au>Tokuyama, Yoko</au><au>Komai, Yoshihiro</au><au>Ohe, Shuichi</au><au>Yanai, Hirotsugu</au><au>Omachi, Taichi</au><au>Ueno, Hiroo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of a Novel Lingual Organoid Culture System: Generation of Organoids Having Mature Keratinized Epithelium from Adult Epithelial Stem Cells</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2013-11-15</date><risdate>2013</risdate><volume>3</volume><issue>1</issue><spage>3224</spage><pages>3224-</pages><artnum>3224</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Despite the strong need for the establishment of a lingual epithelial cell culture system, a simple and convenient culture method has not yet been established. Here, we report the establishment of a novel lingual epithelium organoid culture system using a three-dimensional matrix and growth factors. Histological analyses showed that the generated organoids had both a stratified squamous epithelial cell layer and a stratum corneum. Very recently, we showed via a multicolor lineage tracing method that Bmi1-positive stem cells exist at the base of the epithelial basal layer in the interpapillary pit. Using our new culture system, we found that organoids could be generated by single Bmi1-positive stem cells and that in the established organoids, multiple Bmi1-positive stem cells were generated at the outermost layer. Moreover, we observed that organoids harvested at an early point in culture could be engrafted and maturate in the tongue of recipient mice and that the organoids generated from carcinogen-treated mice had an abnormal morphology. Thus, this culture system presents valuable settings for studying not only the regulatory mechanisms of lingual epithelium but also lingual regeneration and carcinogenesis.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>24232854</pmid><doi>10.1038/srep03224</doi><oa>free_for_read</oa></addata></record> |
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| subjects | 14 14/28 14/35 14/63 631/532 631/80 Adult Stem Cells - cytology Adult Stem Cells - metabolism Adult Stem Cells - physiology Animals Carcinogenesis Carcinogenesis - metabolism Carcinogenesis - pathology Cell culture Cell Culture Techniques - methods Epithelial Cells - cytology Epithelial Cells - metabolism Epithelial Cells - physiology Epithelium Epithelium - metabolism Epithelium - physiology Growth factors Humanities and Social Sciences Lingual epithelium Mice Mice, Inbred C57BL multidisciplinary Organoids Organoids - cytology Organoids - metabolism Organoids - physiology Polycomb Repressive Complex 1 - metabolism Proto-Oncogene Proteins - metabolism Regeneration - physiology Rodents Science Stem cell transplantation Stem cells Stratum corneum Tongue Tongue - cytology Tongue - metabolism Tongue - physiology |
| title | Establishment of a Novel Lingual Organoid Culture System: Generation of Organoids Having Mature Keratinized Epithelium from Adult Epithelial Stem Cells |
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