Dual regulation of Dmc1-driven DNA strand exchange by Swi5-Sfr1 activation and Rad22 inhibition
Both ubiquitously expressed Rad51 and meiosis-specific Dmc1 are required for crossover production during meiotic recombination. The budding yeast Rad52 and its fission yeast ortholog, Rad22, are "mediators;" i.e., they help load Rad51 onto ssDNA coated with replication protein A (RPA). Her...
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| Veröffentlicht in: | Genes & development 2013-11, Vol.27 (21), p.2299-2304 |
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| creator | Murayama, Yasuto Kurokawa, Yumiko Tsutsui, Yasuhiro Iwasaki, Hiroshi |
| description | Both ubiquitously expressed Rad51 and meiosis-specific Dmc1 are required for crossover production during meiotic recombination. The budding yeast Rad52 and its fission yeast ortholog, Rad22, are "mediators;" i.e., they help load Rad51 onto ssDNA coated with replication protein A (RPA). Here we show that the Swi5-Sfr1 complex from fission yeast is both a mediator that loads Dmc1 onto ssDNA and a direct "activator" of DNA strand exchange by Dmc1. In stark contrast, Rad22 inhibits Dmc1 action by competing for its binding to RPA-coated ssDNA. Thus, Rad22 plays dual roles in regulating meiotic recombination: activating Rad51 and inhibiting Dmc1. |
| doi_str_mv | 10.1101/gad.218693.113 |
| format | Article |
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The budding yeast Rad52 and its fission yeast ortholog, Rad22, are "mediators;" i.e., they help load Rad51 onto ssDNA coated with replication protein A (RPA). Here we show that the Swi5-Sfr1 complex from fission yeast is both a mediator that loads Dmc1 onto ssDNA and a direct "activator" of DNA strand exchange by Dmc1. In stark contrast, Rad22 inhibits Dmc1 action by competing for its binding to RPA-coated ssDNA. 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The budding yeast Rad52 and its fission yeast ortholog, Rad22, are "mediators;" i.e., they help load Rad51 onto ssDNA coated with replication protein A (RPA). Here we show that the Swi5-Sfr1 complex from fission yeast is both a mediator that loads Dmc1 onto ssDNA and a direct "activator" of DNA strand exchange by Dmc1. In stark contrast, Rad22 inhibits Dmc1 action by competing for its binding to RPA-coated ssDNA. Thus, Rad22 plays dual roles in regulating meiotic recombination: activating Rad51 and inhibiting Dmc1.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Crossing Over, Genetic - genetics</subject><subject>DNA Breaks, Double-Stranded</subject><subject>DNA Repair - genetics</subject><subject>DNA, Single-Stranded - metabolism</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Homologous Recombination</subject><subject>Meiosis</subject><subject>Protein Binding</subject><subject>Protein Stability</subject><subject>Rad52 DNA Repair and Recombination Protein - metabolism</subject><subject>Recombinases - metabolism</subject><subject>Research Communication</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Schizosaccharomyces pombe</subject><subject>Schizosaccharomyces pombe Proteins - genetics</subject><subject>Schizosaccharomyces pombe Proteins - metabolism</subject><issn>0890-9369</issn><issn>1549-5477</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1P20AQxVcIVELolWO1Ry5O9_vjghSRtiBFVCr0vFrvrpOtHDvs2gH--9oyReXGaTQzv_c0owfABUYLjBH-urF-QbASmg49PQIzzJkuOJPyGMyQ0qjQVOhTcJbzH4SQQEJ8AqeEjRIpZsCselvDFDZ9bbvYNrCt4GrncOFTPIQGru6WMHfJNh6GZ7e1zSbA8gXeP0Ve3FcJQ-u6eJikI_TLekJgbLaxjOPwHJxUts7h82udg9_fvz1c3xTrnz9ur5frwnFKuwKrQGnAmoiqqpTADkkflGcVcp5Z4XHQjJZMEi41KlXJhWDDFFHJuVeI0Dm4mnz3fbkL3oVmOLo2-xR3Nr2Y1kbzftPErdm0B0MVURyLweDy1SC1j33IndnF7EJd2ya0fTaYCUUlwoR8AGWayAEcXRcT6lKbcwrV20UYmTFAMwRopgCHng6CL___8Yb_S4z-BXD4lY4</recordid><startdate>20131101</startdate><enddate>20131101</enddate><creator>Murayama, Yasuto</creator><creator>Kurokawa, Yumiko</creator><creator>Tsutsui, Yasuhiro</creator><creator>Iwasaki, Hiroshi</creator><general>Cold Spring Harbor Laboratory Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20131101</creationdate><title>Dual regulation of Dmc1-driven DNA strand exchange by Swi5-Sfr1 activation and Rad22 inhibition</title><author>Murayama, Yasuto ; 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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Adenosine Triphosphate - metabolism Crossing Over, Genetic - genetics DNA Breaks, Double-Stranded DNA Repair - genetics DNA, Single-Stranded - metabolism DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Homologous Recombination Meiosis Protein Binding Protein Stability Rad52 DNA Repair and Recombination Protein - metabolism Recombinases - metabolism Research Communication Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins - metabolism Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins - genetics Schizosaccharomyces pombe Proteins - metabolism |
| title | Dual regulation of Dmc1-driven DNA strand exchange by Swi5-Sfr1 activation and Rad22 inhibition |
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