Vitamin D Elicits Anti-Inflammatory Response, Inhibits Contractile-Associated Proteins, and Modulates Toll-Like Receptors in Human Myometrial Cells
Infection during pregnancy triggers inflammation, which can increase myometrial contractions and the risk of premature labor and delivery. In this study, we assessed the effects of vitamin D, an anti-inflammatory ligand on cytokines, chemokines, toll-like receptors, and contractile-associated protei...
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Veröffentlicht in: | Reproductive sciences (Thousand Oaks, Calif.) Calif.), 2013-04, Vol.20 (4), p.463-475 |
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description | Infection during pregnancy triggers inflammation, which can increase myometrial contractions and the risk of premature labor and delivery. In this study, we assessed the effects of vitamin D, an anti-inflammatory ligand on cytokines, chemokines, toll-like receptors, and contractile-associated proteins on immortalized human myometrial smooth muscle (UtSM) cells stimulated with lipopolysaccharide (LPS), a bacterial endotoxin, or interleukin (IL)-1β and measured Toll-like receptor (TLR)-10 expression in pregnant myometrial tissues. A superarray analysis revealed downregulation of the chemokines monocyte chemoattractant protein (MCP)-1, Chemokine (C-X-C motif) ligand (CXCL)-10, CXCL-11, and chemokine (C-X3-C motif) ligand (CX3CL)-1; the proinflammatory cytokines IL-13 and tumor necrosis factor (TNF)-α; the TLR-4 and -5 and triggering receptor expressed on myeloid cells (TREM)-2 and upregulation of the anti-inflammatory cytokine IL-10, as well as Toll interacting protein (TOLLIP) and TREM-1 in vitamin D-treated UtSM cells. In the presence of LPS, vitamin D caused dose-dependent decreases in the messenger RNA expression of MCP-1, IL-1β, IL-13, TNF-α, TLR-4, and TLR-5, the contractile-associated proteins connexin 43, the oxytocin receptor, and the prostaglandin receptor but caused increases in IL-10 and TLR-10 in UtSM cells. The TLR-10 expression was higher in human myometrial tissue obtained from women at term not in labor compared to labor. Vitamin D also attenuated IL-1β-induced MCP-1, IL-6, connexin 43, cyclooxygenase (COX)-2, and prostaglandin receptor expression. Western analysis showed that vitamin D decreased MCP-1, TLR-4, and connexin 43 in the presence of LPS and decreased connexin 43 in the presence of IL-1β. Our results suggest that vitamin D can potentially decrease infection-induced increases in cytokines and contractile-associated proteins in the myometrium. |
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In this study, we assessed the effects of vitamin D, an anti-inflammatory ligand on cytokines, chemokines, toll-like receptors, and contractile-associated proteins on immortalized human myometrial smooth muscle (UtSM) cells stimulated with lipopolysaccharide (LPS), a bacterial endotoxin, or interleukin (IL)-1β and measured Toll-like receptor (TLR)-10 expression in pregnant myometrial tissues. A superarray analysis revealed downregulation of the chemokines monocyte chemoattractant protein (MCP)-1, Chemokine (C-X-C motif) ligand (CXCL)-10, CXCL-11, and chemokine (C-X3-C motif) ligand (CX3CL)-1; the proinflammatory cytokines IL-13 and tumor necrosis factor (TNF)-α; the TLR-4 and -5 and triggering receptor expressed on myeloid cells (TREM)-2 and upregulation of the anti-inflammatory cytokine IL-10, as well as Toll interacting protein (TOLLIP) and TREM-1 in vitamin D-treated UtSM cells. In the presence of LPS, vitamin D caused dose-dependent decreases in the messenger RNA expression of MCP-1, IL-1β, IL-13, TNF-α, TLR-4, and TLR-5, the contractile-associated proteins connexin 43, the oxytocin receptor, and the prostaglandin receptor but caused increases in IL-10 and TLR-10 in UtSM cells. The TLR-10 expression was higher in human myometrial tissue obtained from women at term not in labor compared to labor. Vitamin D also attenuated IL-1β-induced MCP-1, IL-6, connexin 43, cyclooxygenase (COX)-2, and prostaglandin receptor expression. Western analysis showed that vitamin D decreased MCP-1, TLR-4, and connexin 43 in the presence of LPS and decreased connexin 43 in the presence of IL-1β. Our results suggest that vitamin D can potentially decrease infection-induced increases in cytokines and contractile-associated proteins in the myometrium.</description><identifier>ISSN: 1933-7191</identifier><identifier>EISSN: 1933-7205</identifier><identifier>DOI: 10.1177/1933719112459225</identifier><identifier>PMID: 23012315</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology ; Cell Line, Transformed ; Cells, Cultured ; Chemokines ; Contractile Proteins - antagonists & inhibitors ; Contractile Proteins - physiology ; Embryology ; Female ; Humans ; Medicine & Public Health ; Myometrium - cytology ; Myometrium - drug effects ; Myometrium - physiology ; Obstetrics/Perinatology/Midwifery ; Original ; Original Article ; Pregnancy ; Reproductive Medicine ; Toll-Like Receptors - biosynthesis ; Toll-Like Receptors - metabolism ; Toll-Like Receptors - physiology ; Vitamin D - pharmacology</subject><ispartof>Reproductive sciences (Thousand Oaks, Calif.), 2013-04, Vol.20 (4), p.463-475</ispartof><rights>The Author(s) 2013</rights><rights>Society for Reproductive Investigation 2013</rights><rights>The Author(s) 2013 2013 Society for Gynecologic Investigation</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c575t-b3fd4f222779b3629308b67787cbfd1618799d244f5b78d98bfc88d73174dfff3</citedby><cites>FETCH-LOGICAL-c575t-b3fd4f222779b3629308b67787cbfd1618799d244f5b78d98bfc88d73174dfff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/1933719112459225$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/1933719112459225$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>230,314,780,784,885,21819,27924,27925,41488,42557,43621,43622,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23012315$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thota, Chandrasekhar</creatorcontrib><creatorcontrib>Farmer, Takeisha</creatorcontrib><creatorcontrib>Garfield, Robert E.</creatorcontrib><creatorcontrib>Menon, Ramkumar</creatorcontrib><creatorcontrib>Al-Hendy, Ayman</creatorcontrib><title>Vitamin D Elicits Anti-Inflammatory Response, Inhibits Contractile-Associated Proteins, and Modulates Toll-Like Receptors in Human Myometrial Cells</title><title>Reproductive sciences (Thousand Oaks, Calif.)</title><addtitle>Reprod. Sci</addtitle><addtitle>Reprod Sci</addtitle><description>Infection during pregnancy triggers inflammation, which can increase myometrial contractions and the risk of premature labor and delivery. In this study, we assessed the effects of vitamin D, an anti-inflammatory ligand on cytokines, chemokines, toll-like receptors, and contractile-associated proteins on immortalized human myometrial smooth muscle (UtSM) cells stimulated with lipopolysaccharide (LPS), a bacterial endotoxin, or interleukin (IL)-1β and measured Toll-like receptor (TLR)-10 expression in pregnant myometrial tissues. A superarray analysis revealed downregulation of the chemokines monocyte chemoattractant protein (MCP)-1, Chemokine (C-X-C motif) ligand (CXCL)-10, CXCL-11, and chemokine (C-X3-C motif) ligand (CX3CL)-1; the proinflammatory cytokines IL-13 and tumor necrosis factor (TNF)-α; the TLR-4 and -5 and triggering receptor expressed on myeloid cells (TREM)-2 and upregulation of the anti-inflammatory cytokine IL-10, as well as Toll interacting protein (TOLLIP) and TREM-1 in vitamin D-treated UtSM cells. In the presence of LPS, vitamin D caused dose-dependent decreases in the messenger RNA expression of MCP-1, IL-1β, IL-13, TNF-α, TLR-4, and TLR-5, the contractile-associated proteins connexin 43, the oxytocin receptor, and the prostaglandin receptor but caused increases in IL-10 and TLR-10 in UtSM cells. The TLR-10 expression was higher in human myometrial tissue obtained from women at term not in labor compared to labor. Vitamin D also attenuated IL-1β-induced MCP-1, IL-6, connexin 43, cyclooxygenase (COX)-2, and prostaglandin receptor expression. Western analysis showed that vitamin D decreased MCP-1, TLR-4, and connexin 43 in the presence of LPS and decreased connexin 43 in the presence of IL-1β. Our results suggest that vitamin D can potentially decrease infection-induced increases in cytokines and contractile-associated proteins in the myometrium.</description><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</subject><subject>Cell Line, Transformed</subject><subject>Cells, Cultured</subject><subject>Chemokines</subject><subject>Contractile Proteins - antagonists & inhibitors</subject><subject>Contractile Proteins - physiology</subject><subject>Embryology</subject><subject>Female</subject><subject>Humans</subject><subject>Medicine & Public Health</subject><subject>Myometrium - cytology</subject><subject>Myometrium - drug effects</subject><subject>Myometrium - physiology</subject><subject>Obstetrics/Perinatology/Midwifery</subject><subject>Original</subject><subject>Original Article</subject><subject>Pregnancy</subject><subject>Reproductive Medicine</subject><subject>Toll-Like Receptors - biosynthesis</subject><subject>Toll-Like Receptors - metabolism</subject><subject>Toll-Like Receptors - physiology</subject><subject>Vitamin D - pharmacology</subject><issn>1933-7191</issn><issn>1933-7205</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9vFCEYxidGY2v17slw9NDRAYYBLiabtdpNttGY6pUw_NlSGViBMdnP4ReWzW4b9aCeIO_zex7el7dpnsPuFYSUvoYcYwo5hKgnHCHyoDndl1qKOvLw7l71k-ZJzrddR3qO2OPmBOEOIgzJafPjiytycgG8BRfeKVcyWITi2lWwXk6TLDHtwCeTtzFkcw5W4caNe2gZQ0lSFedNu8g5KieL0eBjisW4kM-BDBpcRT37Ws_gOnrfrt1XU7OU2dbUDOqjl_MkA7jaxcmU5KQHS-N9fto8stJn8-x4njWf311cLy_b9Yf3q-Vi3SpCSWlHbHVvEUKU8hEPiOOOjQOljKrRajhARjnXqO8tGSnTnI1WMaYphrTX1lp81rw55G7ncTJamf1IXmyTm2TaiSid-F0J7kZs4neBGcKYkxrw8hiQ4rfZ5CIml1UdQQYT5yxgP_SkY4QO_0ZrVwwTAnFFuwOqUsw5GXvfEezEfu3iz7VXy4tfJ7k33O25AvAA5CqFjUniNs4p1N_9W2h79MiN-Q_-J39wxfw</recordid><startdate>20130401</startdate><enddate>20130401</enddate><creator>Thota, Chandrasekhar</creator><creator>Farmer, Takeisha</creator><creator>Garfield, Robert E.</creator><creator>Menon, Ramkumar</creator><creator>Al-Hendy, Ayman</creator><general>SAGE Publications</general><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope><scope>5PM</scope></search><sort><creationdate>20130401</creationdate><title>Vitamin D Elicits Anti-Inflammatory Response, Inhibits Contractile-Associated Proteins, and Modulates Toll-Like Receptors in Human Myometrial Cells</title><author>Thota, Chandrasekhar ; Farmer, Takeisha ; Garfield, Robert E. ; Menon, Ramkumar ; Al-Hendy, Ayman</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c575t-b3fd4f222779b3629308b67787cbfd1618799d244f5b78d98bfc88d73174dfff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</topic><topic>Cell Line, Transformed</topic><topic>Cells, Cultured</topic><topic>Chemokines</topic><topic>Contractile Proteins - antagonists & inhibitors</topic><topic>Contractile Proteins - physiology</topic><topic>Embryology</topic><topic>Female</topic><topic>Humans</topic><topic>Medicine & Public Health</topic><topic>Myometrium - cytology</topic><topic>Myometrium - drug effects</topic><topic>Myometrium - physiology</topic><topic>Obstetrics/Perinatology/Midwifery</topic><topic>Original</topic><topic>Original Article</topic><topic>Pregnancy</topic><topic>Reproductive Medicine</topic><topic>Toll-Like Receptors - biosynthesis</topic><topic>Toll-Like Receptors - metabolism</topic><topic>Toll-Like Receptors - physiology</topic><topic>Vitamin D - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thota, Chandrasekhar</creatorcontrib><creatorcontrib>Farmer, Takeisha</creatorcontrib><creatorcontrib>Garfield, Robert E.</creatorcontrib><creatorcontrib>Menon, Ramkumar</creatorcontrib><creatorcontrib>Al-Hendy, Ayman</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Reproductive sciences (Thousand Oaks, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thota, Chandrasekhar</au><au>Farmer, Takeisha</au><au>Garfield, Robert E.</au><au>Menon, Ramkumar</au><au>Al-Hendy, Ayman</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vitamin D Elicits Anti-Inflammatory Response, Inhibits Contractile-Associated Proteins, and Modulates Toll-Like Receptors in Human Myometrial Cells</atitle><jtitle>Reproductive sciences (Thousand Oaks, Calif.)</jtitle><stitle>Reprod. Sci</stitle><addtitle>Reprod Sci</addtitle><date>2013-04-01</date><risdate>2013</risdate><volume>20</volume><issue>4</issue><spage>463</spage><epage>475</epage><pages>463-475</pages><issn>1933-7191</issn><eissn>1933-7205</eissn><abstract>Infection during pregnancy triggers inflammation, which can increase myometrial contractions and the risk of premature labor and delivery. In this study, we assessed the effects of vitamin D, an anti-inflammatory ligand on cytokines, chemokines, toll-like receptors, and contractile-associated proteins on immortalized human myometrial smooth muscle (UtSM) cells stimulated with lipopolysaccharide (LPS), a bacterial endotoxin, or interleukin (IL)-1β and measured Toll-like receptor (TLR)-10 expression in pregnant myometrial tissues. A superarray analysis revealed downregulation of the chemokines monocyte chemoattractant protein (MCP)-1, Chemokine (C-X-C motif) ligand (CXCL)-10, CXCL-11, and chemokine (C-X3-C motif) ligand (CX3CL)-1; the proinflammatory cytokines IL-13 and tumor necrosis factor (TNF)-α; the TLR-4 and -5 and triggering receptor expressed on myeloid cells (TREM)-2 and upregulation of the anti-inflammatory cytokine IL-10, as well as Toll interacting protein (TOLLIP) and TREM-1 in vitamin D-treated UtSM cells. In the presence of LPS, vitamin D caused dose-dependent decreases in the messenger RNA expression of MCP-1, IL-1β, IL-13, TNF-α, TLR-4, and TLR-5, the contractile-associated proteins connexin 43, the oxytocin receptor, and the prostaglandin receptor but caused increases in IL-10 and TLR-10 in UtSM cells. The TLR-10 expression was higher in human myometrial tissue obtained from women at term not in labor compared to labor. Vitamin D also attenuated IL-1β-induced MCP-1, IL-6, connexin 43, cyclooxygenase (COX)-2, and prostaglandin receptor expression. Western analysis showed that vitamin D decreased MCP-1, TLR-4, and connexin 43 in the presence of LPS and decreased connexin 43 in the presence of IL-1β. Our results suggest that vitamin D can potentially decrease infection-induced increases in cytokines and contractile-associated proteins in the myometrium.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>23012315</pmid><doi>10.1177/1933719112459225</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Anti-Inflammatory Agents, Non-Steroidal - pharmacology Cell Line, Transformed Cells, Cultured Chemokines Contractile Proteins - antagonists & inhibitors Contractile Proteins - physiology Embryology Female Humans Medicine & Public Health Myometrium - cytology Myometrium - drug effects Myometrium - physiology Obstetrics/Perinatology/Midwifery Original Original Article Pregnancy Reproductive Medicine Toll-Like Receptors - biosynthesis Toll-Like Receptors - metabolism Toll-Like Receptors - physiology Vitamin D - pharmacology |
title | Vitamin D Elicits Anti-Inflammatory Response, Inhibits Contractile-Associated Proteins, and Modulates Toll-Like Receptors in Human Myometrial Cells |
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